Detection of polymorphisms for part of Bn.FAE1-C3 using EcoTILLING. DNA from each accession mixed 1:1 with B. napus cv. Huayou5 and DNA from each accession alone were used for EcoTILLING, and these two samples were loaded on adjacent lanes on the gel. Lanes with triangle indicate the mixed samples for testing. New bands appeared in both lanes, indicating that the polymorphism was heterozygous. The sizes of DNA ladders and digested PCR products are labelled with corresponding numbers and the unit is bp.