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Table 2 Specific activity of chitinase preparations

From: Differential expression of maize chitinases in the presence or absence of Trichoderma harzianum strain T22 and indications of a novel exo- endo-heterodimeric chitinase activity

  MUB based Activity MUA based Activity
  Control Trichoderma inoculated Control Trichoderma inoculated
Total extracted protein 0.033
± 0.0069
0.035
± 0.0037
0.40
± 0.054
0.40
± 0.04
Rotofor fraction 4 0.73
± 0.0036
0.48
± 0.0022
0.33
± 0
0.73
± 0.052
Purified dimeric protein 9.97
± 0.198
16.88
± 0.34
1.1
± 0.0126
2.04
± 0
Fold enrichment 302 482 2.8 5.1
  1. Specific activity units are μM MU*min-1*μg-1. Measurements were performed as described in materials and methods using MUA and MUB separately. Known protein amounts from each preparation were taken for the assay.