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Table 2 Specific activity of chitinase preparations

From: Differential expression of maize chitinases in the presence or absence of Trichoderma harzianum strain T22 and indications of a novel exo- endo-heterodimeric chitinase activity

 

MUB based Activity

MUA based Activity

 

Control

Trichoderma inoculated

Control

Trichoderma inoculated

Total extracted protein

0.033

± 0.0069

0.035

± 0.0037

0.40

± 0.054

0.40

± 0.04

Rotofor fraction 4

0.73

± 0.0036

0.48

± 0.0022

0.33

± 0

0.73

± 0.052

Purified dimeric protein

9.97

± 0.198

16.88

± 0.34

1.1

± 0.0126

2.04

± 0

Fold enrichment

302

482

2.8

5.1

  1. Specific activity units are μM MU*min-1*μg-1. Measurements were performed as described in materials and methods using MUA and MUB separately. Known protein amounts from each preparation were taken for the assay.
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BMC Plant Biology

ISSN: 1471-2229

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