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Table 2 Primer sequences used for the amplification of SPSII gene in wheat and other related grasses

From: Delineating the structural, functional and evolutionary relationships of sucrose phosphate synthase gene family II in wheat and related grasses

Forward primer (5'-3')

Reverseprimer (5'-3')

PCR profile

A. Triticum aestivum- A genome

 

1

1. w-spsII-207L* (CGTTTTATCCCCCCATAAGAGA)

w-spsII-5R (CCCAAATGGTTCAATATAAGCACA)

 

2. w-spsII-5L (AGCACAAATGGAGCTGTTTTG)

w-spsII-130R* (AATATCCCTCAAAGAGTCACCAC)

 

3. w-spsII-9L (GCTTATGGTCTACCTATGGTTGCT)

w-spsII-kR* (ATAGTAGGGAAGGTTGAGAAAATCA)

 

4#. w-spsII-dL* (AGAAAAGAATAGCGAGAGTGGAA)

w-spsII-24R (AGATGCTAATTATTCGTAGAGATGCAT)

 

5a.w-spsII-dL (AGAAAAGAATAGCGAGAGTGGAA)

w-spsII-hR (ATGCTTGAATTCTCATTGTCTTC)

 

B. Triticum aestivum -B genome

 

1

1. w-spsII-205L (AGCTGCTACGGTCGTGAAATG)

w-spsII-210R* (CTCTTATGGGGGGATAAAAAT)

 

2#. w-spsII-65L (CATTTGGTGAACACCATGAGCTA)

w-spsII-73R* ( CCGGTGTATATCGACAGGT)

 

3. w-spsII-2L (GCTGGTTAAGGCATTTGGT)

w-spsII-108R* (AACTCCTTCCATATTTCGACATGT)

 

4. w-spsII-86L* (GAAAAATCAGGCAACATGTCG)

w-spII-14R (TGCTTGGGATCGTGATGCT)

 

5. w-spsII-iL (CACTAAGGTTTGGTTATTCCTATAACTGT)

w-spsII-nR * (CCTGCACAAAGATGAACAAACG)

 

6#. w-spsII-mL* (AACCACAAAGTCCTGATGGTACG)

w-spsII-24R (AGATGCTAATTATTCGTAGAGATGCAT)

 

7#. w-spsII-12L (AAACTATTTGTCACGGGTTGGT)

w-spsII-nR (CCTGCACAAAGATGAACAAACG)

 

8a.w-spsII-wL (GTCGTTTGACACCTCATTGTTG)

w-spsII-nR (CCTGCACAAAGATGAACAAACG)

 

C. Triticum aestivum -D genome

 

2

1 w-spsII-D1L* (CCAAAATGGTGGGCCTGTTG)

w-spsII-14R (TGCTTGGGATCGTGATGCT)

 

2a. w-spsII-D1L (CCAAAATGGTGGGCCTGTTG)

w-spsII-D11R GAAACTTCAGTAGCATCATCACTCTTTG)

 

D. Triticum urartu , Triticum speltoides , Aegilops tauschii , Hordeum vulgare

 

1

1. w-spsII-205L (AGCTGCTACGGTCGTGAAATG)

w-spsII-3R (TGGAACTTCAGATTGCTTATGG)

 

2. w-spsII-2L (GCTGGTTAAGGCATTTGGT)

w-spsII-1R (GGTGAGCCCAAATGGTTCAATATA)

 

3. w-spsII-5L (AGCACAAATGGAGCTGTTTTG)

w-spsII-40R (CCCGGTGTATATCGACAGGC)

 

4. w-spsII-9L (GCTTATGGTCTACCTATGGTTGCT)

w-spsII-17R (GATGTTGACAATACAAAACCAAGAGC)

 

5. w-spsII-22L (TTCATACCTGTTCTAGCATCACGA)

w-spsII-23R (GGAACCTATGAACAATTTCACCA)

 

6. w-spsII-14L (GAGCAGCATCAGAGATACATCCTT)

w-spsII-14R (TGCTTGGGATCGTGATGCT)

 

7. w-spsII-20L (AAAGCTGGGGCCACCAC)

w-spsII-20R (TCGTGATGCTAGAACAGGTATGAA)

 

8. w-spsII-22L (TTCATACCTGTTCTAGCATCACGA)

w-spsII-24R (AGATGCTAATTATTCGTAGAGATGCAT)

 

9. w-spsII-22L (TTCATACCTGTTCTAGCATCACGA)

w-spsII-31R (ACCGTCATGTTCGACAGCTCTAC)

 

10. w-spsII-11L (AATCAAAATGATATAGCTGAGGCACTT)

w-spsII-17R (GATGTTGACAATACAAAACCAAGAGC)

 

11. w-spsII-15L (GTTAACATCTGGGGGCATAGAAAT)

w-spsII-20R (TCGTGATGCTAGAACAGGTATGAA)

 

12. w-spsII-5L (AGCACAAATGGAGCTGTTTTG)

w-spsII-9R (CACCATTTTGGGTAGCAACC)

 
  1. *represents homoeologue-specific primers ; arepresents primer pairs used for quantitative real time PCR; # represents primer pairs used for genetic mapping. For details of PCR profile please see methods section
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BMC Plant Biology

ISSN: 1471-2229

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