Figure 4

Genome-specific LKR/SDH primers. Common priming sites for all three genomes are F3 and R3. Genome-specific priming sites were developed in intron 22 for the B- and D-genomes (BF3 and DF3, respectively) and in exon 23 for the A-genome (AF3). (B) PCR products generated with each genome-specific LKR/SDH primer plus common primer R3 from genomic DNA of hexaploid wheat cv Chinese Spring group 6 chromosome nullisomic-tetrasomic genetic lines (N6A = nulli6A, N6B = nulli6B, N6D = nulli6D). Two amounts of sample were loaded for each DNA.