Identification of transcription-factor genes expressed in the Arabidopsis female gametophyte

BMC Plant Biology

Table 1 Summary of qRT-PCR and promoter-fusion analyses to identify transcription-factor genes expressed in the female gametophyte.

The genes are ranked in descending order based on the average ΔΔC_T value in the secondary qRT-PCR screen. Average ΔΔC_T values and P values (Student's t-test) were calculated as described in Methods. GFP activity in female gametophytes was analyzed at one day after emasculation (corresponding to the mature stage of female gametophyte development, FG7 [63]) and in early-developing seeds at flower stage 16 (corresponding to endosperm stages V to VI and embryo stages from elongated zygote to two-cell embryo-proper [68, 69]). A, antipodal cells; C, central cell; E, egg cell; EM, embryo; EN, endosperm; ne, no consistent expression; nd, not determined; S, synergid cells; (), weak expression.
¹ Arabidopsis Genome Initiative number.
² Based on the classification of Arabidopsis transcription-factor genes provided by AGRIS http://arabidopsis.med.ohio-state.edu/AtTFDB and DATF http://datf.cbi.pku.edu.cn.
³ Based on gene annotation provided by TAIR http://www.arabidopsis.org.
⁴ GFP activity was more intense in the micropylar endosperm compared to the chalazal and peripheral endosperm.
⁵ GFP activity was not detected at FG7 but was detected at FG5.

ISSN: 1471-2229