Figure 4
From: The Protein Disulfide Isomerase gene family in bread wheat (T. aestivum L.)
Chromosome location of wheat PDI-like genes. (A-G) Southern analysis of DNA of CS and its NT lines of homoeologous groups 7 (A), 1 (B), 5 (C), 4 (D), 2 (E and G) and 6 (F) digested with NcoI (A), SacI (B), EcoRV (C), HindIII (D and G), EcoRI (E) and DraI (F) and hybridised with specific probes of seven PDI-like sequences: TaPDIL3-1 (A), TaPDIL4-1 (B), TaPDIL5-1 (C), TaPDIL6-1 (D), TaPDIL7-1 (E), TaPDIL7-2 (F) and TaPDIL8-1 (G). The deduced chomosome location is indicated on the left side. The sizes of the molecular-weight marker (kb) are shown on the right side. (H) Gel electrophoresis in a 2% agarose gel of PCR products amplified from DNA of CS and its NT lines of homoeologous group 6 with a specific primer pair designed at the 3' region of the TaPDIL2-1sequence. The deduced chromosome location is indicated on the left side. Size of the molecular-weight marker bands (kb) are shown on the right side.