Complementation of a phycocyanin-bilin lyase from Synechocystis sp. PCC 6803 with a nucleomorph-encoded open reading frame from the cryptophyte Guillardia theta
- Kathrin Bolte†1,
- Oliver Kawach†1,
- Julia Prechtl†1,
- Nicole Gruenheit2,
- Julius Nyalwidhe3 and
- Uwe-G Maier1Email author
© Bolte et al; licensee BioMed Central Ltd. 2008
Received: 14 December 2007
Accepted: 16 May 2008
Published: 16 May 2008
Cryptophytes are highly compartmentalized organisms, expressing a secondary minimized eukaryotic genome in the nucleomorph and its surrounding remnant cytoplasm, in addition to the cell nucleus, the mitochondrion and the plastid. Because the members of the nucleomorph-encoded proteome may contribute to essential cellular pathways, elucidating nucleomorph-encoded functions is of utmost interest. Unfortunately, cryptophytes are inaccessible for genetic transformations thus far. Therefore the functions of nucleomorph-encoded proteins must be elucidated indirectly by application of methods in genetically accessible organisms.
Orf222, one of the uncharacterized nucleomorph-specific open reading frames of the cryptophyte Guillardia theta, shows homology to slr1649 of Synechocystis sp. PCC 6803. Recently a further homolog from Synechococcus sp. PCC 7002 was characterized to encode a phycocyanin-β155-bilin lyase. Here we show by insertion mutagenesis that the Synechocystis sp. PCC 6803 slr1649-encoded protein also acts as a bilin lyase, and additionally contributes to linker attachment and/or stability of phycobilisomes. Finally, our results indicate that the phycocyanin-β155-bilin lyase of Synechocystis sp. PCC 6803 can be complemented in vivo by the nucleomorph-encoded open reading frame orf222.
Our data show that the loss of phycocyanin-lyase function causes pleiotropic effects in Synechocystis sp. PCC 6803 and indicate that after separating from a common ancestor protein, the phycoerythrin lyase from Guillardia theta has retained its capacity to couple a bilin group to other phycobiliproteins. This is a further, unexpected example of the universality of phycobiliprotein lyases.
Phycobiliproteins are subunits of the major accessory light-harvesting complexes (LHC) of most cyanobacteria and red alga and are present in the thylakoid lumen of cryptophytes as well. Covalently linked to the proteins are chromophore groups, the phycobilins [1, 2]. These open tetrapyrrole rings are coupled to conserved cysteine residues via a thioether bond and are necessary for light harvesting and efficient energy flow . Various phycobiliproteins, namely allophycocyanin, phycocyanin, phycoerythrin, phycoerythrocyanin, carry different numbers of bilin groups.
Attachment of bilins to phycobiliproteins is an enzymatically catalyzed reaction, which also occurs spontaneously, but at low efficiency . Several bilin-attaching lyases are described. One of the dimeric enzymes encoded by cpcE and cpcF genes links the chromophore to the phycocyanin α-subunit [4, 5]. PecE and pecF genes encode the second known lyase, specific for the phycoerythrocyanin α-subunit [6–8]. Recently Zhao and co-workers discovered that a CpeS-like protein functions as a phycocyanobilin-cysteine-beta84 lyase in Anabaena sp. PCC 7120, which was the first lyase identified for a β-subunit of a phycobiliprotein . Another lyase specific for a β-subunit of a phycobiliprotein was found by Shen et al. . They identified the gene product of cpcT to be a Cys-β153-phycocyanobilin lyase in Synechococcus sp. PCC 7002. Moreover, Zhao et al. reported the Anabaena sp. PCC 7120 CpeS1 as a "near-universal" lyase for cysteine-84-binding sites in cyanobacterial phycobiliproteins [11, 12].
In most cyanobacteria and red algae phycobiliproteins are organized in multimeric complexes, called phycobilisomes [13–15]. Their antenna structure, located on the cytoplasmic surface of the thylakoid membrane, consists of various linker polypeptides and phycobiliproteins. Each phycobilisome is on its part a multimeric complex, composed of a core and several rod structures. Phycobilisomes can be subdivided according to their structure. The most common type in cyanobacteria, the hemidiscoidal one, consists of a tricylindrical core and six rods.
Allophycocyanin (AP, λmax = 650 nm) forms the core structure, connecting the phycobilisomes to the thylakoid membrane via linker proteins. Rods can be composed of three different phycobiliproteins: phycocyanin (PC, λmax = 617 nm) is located proximal to the core, whereas phycoerythrin (PE, λmax = 560 nm) and phycoerythrocyanin (PEC, λmax = 575 nm) are located distal to the core [16, 17]. The phycobilisome rods of each organism differ in their phycobiliprotein composition. Synechocystis sp. PCC 6803 harbors hemidiscoidal phycobilisomes. PC, the only biliprotein in the rod structures in this organism, is composed of α- and β-subunits. These subunits dimerize to heterodimers, assemble to hexameric (αβ)6 discs, and are subsequently coupled to each other, as well as to the AP-core via linker proteins [18, 19]. Depending on their location (in core or rods), and their molecular mass, linker proteins are divided into four groups [20, 21]. Beside their main function of mediating the assembly and stability of the phycobilisomes, linker proteins also promote energy transfer towards the reaction centres .
Guillardia theta is a cryptophyte possessing phycoerythrin as a phycobiliprotein. The β-subunit is encoded on the plastid genome , whereas the phycoerythrin α-subunits are encoded by a nuclear-located gene family. In the latter case, the genes encode preproteins containing a tripartite topogenic signal responsible for the translocation across five biological membranes . Because a wide range of genomic data exists from this unicellular phototrophic organism, existing knowledge can be used to reconstruct the biochemistry of these organisms. The elucidation of protein functions encoded by open reading frames in the nucleomorph genome of Guillardia theta is of special interest, as this genome is minimized and should therefore encode only essential proteins. After analyzing the nucleomorph genome data, Orf222 was identified as being homologous to a number of proteins including Slr1649 from Synechocystis sp. PCC 6803 and CpcT from Synechococcus sp. PCC 7002. Because cryptophytes are inaccessible to genetic manipulations, we created a slr1649-loss-of-function strain of Synechocystis sp. PCC 6803 and complemented this strain with the nucleomorph-encoded orf.
The generated Slr1649 loss-of-function mutant generally has characteristics conductive with the description by Shen et al. for a cpcT knock-out in Synechococcus sp. PCC 7002 . Nevertheless, additional effects in the slr1649 knock-out mutant of Synechocystis sp. PCC 6803 were identified in respect to linker proteins within the phycobilisomes of the mutant. Complementation of slr1649 with the nucleomorph-specific orf222 indicated that the cryptophytic protein, although having originated from an organism using phycoerythrin as accessory pigment, attaches a bilin to the position Cys-β155 of phycocyanin in the cyanobacterium.
Tabular comparison of prokaryotic and eukaryotic homologous of Orf222
Homologous of Orf222
Genomic context of the encoded genes
S. sp. PCC 6803
cpeA /cpeT /cpeY
Nostoc punctiformes PCC 73102
thrC/ npun02004130 /npun02004132
cpeS/ cpeT /cpeR
Anabaena sp. PCC7120
Anabaena variabilis ATCC 29413
S. elongates PCC 6301
S.sp. PCC 7002
S.sp. CC 9311
sync_0484/cpcF/cpcE/ sync_0487 /cpcA/cpcB/pebB
S. elongates PCC 7942
S. sp. CC 9605
cpeS/ cpeT /cpeR
cpcB/cpcA/ syncc9605_0419 /phycocyanobilin- lyase
S. sp. WH 8102
rpcB/rpcA/ synw2024 /phycocyanobilin-lyase
cpeC/mpeD/cpeE/cpeS/ cpeT /cpeR
S. sp. CC9902
cpcB/cpcA/ 9902_1910 /phycocyanobilin-lyase
cpeS/ cpeT /cpeR
cyp/cyp/ tery0543 /transposase
cpeZ/ cpeT /cpeF
cpeS/ cpeT /cpeR
apcD/ glr1182 /cpcB/cpcA
All adjacent to the α- and β- subunit of phycoerythrin
host = Synechococcus
Additionally, clear affiliations of the Gloeobacter violaceus PCC 7421 (glr1182) and Synechococcus sp. PCC 7002 (CpcT) sequences can not be extrapolated. With the exeption of the Prochlorococcus species, at least one member of the Slr1649-like group is present in all cyanobacteria investigated to date. CpeT-like proteins were only detected in cyanobacteria encoding phycoerythrin and/or phycoerythrocyanin. Although the proteins of both groups seem to have the same function, further investigations on the corresponding genes relevant in the genomic context revealed a noticeable difference. Unlike the genes of the cpeT-group, the slr1649-group is by far less conserved in its genomic localization (Table 1). Except for Nostoc sp. PCC 7120, Anabaena variabilis ATCC 29413 and Trichodesmium erythraeum IMS 101, the localization of the homolog gene is always downstream of cpeS. In few cases it is followed by cpeR. On the other hand, genes for the slr1649-group are rather randomly distributed in the investigated cyanobacterial genomes (Table 1).
Generation of a slr1649knock-out strain
We used Synechocystis sp. PCC 6803 as a model organism and created first a slr1649 knockout strain (Δslr1649) by inserting a kanamycin resistance cassette into the slr1649 open reading frame via homologous recombination. The generated homozygous knock-out mutant showes identical features described in Shen et al. . Just like the characterized cpcT mutant in Synechococcus sp. PCC 7002, our knock-out mutant contains a decreased level of phycocyanin up to 60% and a resulting pale green phenotype. The knock-out cells produce smaller phycobilisomes, which could be the cause of their different migration behaviour in sucrose density gradients in comparison to wild type phycobilisomes. Furthermore, isolated phycobilisomes showed a red-shifted absorbance maxima and a slightly smaller apparent molecular mass in the β-subunit of phycocyanin on SDS-PAGE (data not shown). After the digestion of purified phycocyanin with formic acid and a phycocyanobilin addition assay, Shen et al. concluded after digestion that the cpcT gene from Synechocccus sp. PCC 7002 encodes a bilin lyase responsible for the attachment of phycocyanobilin to Cys-153 on the β-subunit of phycocyanin . The same is most likely true for the Synechocystis homolog Slr1649 due to the high homology and the similar phenotype between the two knock-out mutants. Thus, Slr1649 is thought to attach a bilin group to the homolog position Cys-155 of β-phycocyanin in Synechocystis sp.PCC 6803.
Isolation and analysis of phycobilisomes
Upon further investigations, the absence of the linker proteins was proven not to be a transcriptional effect. In exemplarily reverse transcription experiments for cpcC2, the presence of identical cpcC2 transcripts was confirmed in both the mutant and the wild type strain by sequencing the obtained RT-PCR products (data not shown).
Characterization of the complemented strain
Cryptophytes are important organisms for several reasons. In terms of cell biology, their complex compartmentalization is of major interest, because several plasmas and genomes coexist in these organisms, which can be traced back to either a prokaryote or a eukaryote . One of the hallmarks of cryptophytes is the remnant of a second nucleus, which originated by the reduction of the cell nucleus of an engulfed phototrophic eukaryote by another eukaryotic cell . This compartment, the nucleomorph, is minimized in its coding capacity and expresses – in the case of Guillardia theta – only approximately a tenth of that of the E. coli K12 genome . The reduced coding capacity leads to the impression that the genes are still present in the nucleomorph may encode important functions. Thus, we are interested in addressing the functions of proteins encoded by the nucleomorph. However, due to the lack of a method of transfecting cryptophytes, we are studying homologs of the nucleomorph genes and their encoded proteins in genetically accessible organisms in order to identify the functions of the cryptophytic proteins indirectly. One of the best-studied and genetically accessible cyanobacterium is Synechocystis sp. PCC 6803.
Orf222 is one of the uncharacterized nucleomorph-specific open reading frames, for which homologs are present in many cyanobacteria. Analysis of the contribution of this gene within different organisms indicated that a clear correlation between orf222-homolog genes and phycobiliproteins is present, because at least one orf222 homolog is encoded in all organisms expressing phycobiliproteins, including red alga. Phylogenetic studies demonstrated that homologs of the orf222 gene can be classified into the following four groups (Fig. 1): Slr1649-like a, Slr1649-like b, CpeT-like a and CpeT-like b. Because the method for network construction as well as sampling in our studies is different from that of a recently presented phylogeny , it is not surprising that slightly different affiliations are resolved. However, our network corrects erroneous affiliations and indicates uncertainties of the basal grouping. This may be seen in the position of the bacteriophage sequence, which is in the network presented here in the neighbourhood of the bacteria they infect and not in the same branch as the cryptophyte sequence.
Despite the high degree of homology, the members of Slr1649-like and CpeT-like groups differ in the genomic context of the corresponding genes (Table 1). Members of the CpeT group are predominantly localized in the phycoerythrin associated linker protein operon [30, 31] next to the cpeS gene. In some cases, even the cpeR gene is localized directly downstream of cpeT. Because operon structures connect functionally related genes in many cases, CpeT could be an either structurally or functionally moiety of the phycobilisome and it has been shown to be responsible for the attachment of a bilin group to a specific site from β-phycocyanin . It is remarkable that a congruent distribution of members of the Slr1649-groups is not visible, because the genes seem to be localized randomly throughout different genomes. Interestingly, slr1649-homologs exist in some higher plants such as Oryza sativa and Arabidopsis thaliana (Fig. 1). The encoded proteins of these land plants are characterized by a DUF1001 domain as well, but obviously have paralogous functions, since the Arabidopsis thaliana homolog seems to be required for plastid division . It is also suggested to play an important role in cell differentiation and the regulation of the cell division plane in plants . The same could be true for the copy of the bacteriophage S-PM2, but seems to be unlikely since this phage infects different Synechococcus strains and its resource of the homolog may be the result of a selective advantage.
The homozygous knock-out mutant Δslr1649 in Synechocystis sp. PCC 6803 showed features identical to a cpcT knock-out mutant from Synechococcus sp. PCC 7002 described in Shen et al. . Here, the same pale green phenotype and a reduced phycocyanin content, resulting from a missing bilin group in phycobilisomes, was created by knock-out of cpcT, homolog to slr1649 homolog in this cyanobacterium. This indicates that the lyase function of the homologous proteins of Synechococcus sp. PCC 7002 and Synechocystis sp. PCC 6803 is comparable.
Nevertheless, we obtained one additional, not described feature in the Synechocystis sp. PCC 6803 knock-out mutant. Two linker proteins, CpcC2 and CpcD, were missing from the phycobilisomes in the knock-out mutant Δslr1649. CpcD is a small linker (10 kDa) located at the distal tip of rods, possibly functioning as a rod terminating factor . The CpcC2 rod linker (30 kDa) connects the most distal located phycocyanin discs . Both genes are located in the phycocyanin operon from which they are co-transcribed with the phycocyanin subunits and the cpcC1 linker gene . A transcriptional effect causing the loss of the linker proteins appears to be very unlikely, because the α-subunit, the β-subunit and CpcC1 linker are present, although the CpcD and the CpcC2 linker are simultaneously absent. This is indicative of our finding that the cpcC2 gene is indeed transcribed in the mutant as indicated by reverse transcription experiments (data not shown). Therefore, the deficit of the two linker proteins in mutant phycobilisomes is a post-transcriptional effect. However, we can not rule out that a decreased stability of phycobilisomes caused by the altered β-phycocyanin may be the reason for the lack of the two linker proteins in our preparations. In any case, the lack of the linker proteins is a molecular marker for the loss of lyase function, which may be interested to be studied in Synechococcus sp. PCC 7002  as well.
Guillardia theta, the cryptophyte on which we are primarily focusing expresses a homolog of slr1649 in association with phycoerythrin. Phycobiliproteins are located in the thylakoid lumen and apparently not organized in phycobilisomes in cryptophytes. Because the cryptophyte Guillardia theta uses phycoerythrin and not phycocyanin as an accessory pigment for photosynthesis, one might not expect that the putative cryptophytic lyase is able to complement the one of Synechocystis sp. PCC 6803. Surprisingly, the complemented strain showed wild type phycobilisomes structures as shown by the correct attachment of chromophore groups and the linker protein spectrum. Thus, Orf222 from the cryptophyte is able to complement the loss-of-function of Slr1649, indicating that the cryptophytic phycoerythrin lyase has still retained the capacity to couple a bilin group to β-phycocyanin, even after the progenitor of both classes of proteins evolved into apparently paralogous ones. However, a pleiotropic function of a biliprotein lyase with a specificity for phycobilin:cysteine-84 was recently shown in vitro for CpeS1 from Anabaena PCC 7120 , implicating that a multiplicity of proteins like the cryptophytic phycobilin:cysteine-β155 lyase has the capacity to couple bilins to homologous positions in a variety of phycobiliproteins.
Loss-of function of a bilin lyase leads to a variety of effects in phycobilisome structure. This is already shown for a cpcT mutant in Synechococcus sp. PCC 7002 and could be confirmed by the generation of a slr1649 knock-out mutant in Synechocystis sp. PCC 6803, homolog cpcT. One additional feature, the lack of two distal linker proteins, fits with the already known altered phycobilisome structure and may be the reason for the decreased phycocyanin content in mutant missing the bilin lyase.
Loss of Slr1649 was complemented in vivo by the homolog Orf222, which is encoded by the tiny vestigial nucleus of the eukaryotic endosymbiont from the cryptophyte Guillardia theta. Thus, Orf222 is supposed to be a phycoerythrin-bilin lyase in cryptophytes. Despite having originated from an organism using phycoerythrin as its accessory pigment, the protein still has the capacity to couple a chromophore group to the β-subunit of phycocyanin, indicating the functional universality of bilin lyases on the one hand and demonstrating the importance of nucleomorph-encoded cellular functions on the other.
Synechocystis sp. PCC 6803 strains, wild type, Δslr1649 and the complemented strain, were grown at 30°C in Erlenmeyer flasks containing BG-11 media  with gentle swirling under standard light conditions (70 μE) and atmospheric CO2 levels. For growth on plates, BG-11 medium was supplemented with 1% Agar. Plates were incubated under the same conditions as liquid cultures.
Construction of the Δslr1649Mutant
Two pairs of primers were used to amplify the flanking regions of the knock-out construct: 1649a_f (5'-GGT TAC TGC TCG AGG CGC ATC A-3') and 1649a_r (5'-GGA CGG CAA GGG ATC CTA TCT GG-3') generate fragment slr1649a, 1649b_f (5'-GGA CGG CAA GGG ATC CTA TCT GG-3') and 1649b_r (5'-CAG AAA TTG CCG CGG CCA ATC TC-3') fragment 1649b. Both were ligated into the pGEM-T vector (Promega, Mannheim) and after verification of the sequence, transferred into the pBluescript II SK (Stratagene, Amsterdam) vector. Escherichia coli strain MRF' XL-1 blue was used as plasmid host for cloning steps.
Using the BamHI restriction site (inserted by the primer 1649a-r and 1649b_f), a kanamycin resistance gene was cloned between the two fragments resulting in plasmid pΔ1649. After transforming into wild type Synechocystis sp. PCC 6803 cells with this plasmid, transformants were selected on BG-11 agar plates supplemented with kanamycin (5 μg/ml starting concentration). Kanamycin resistant clones were transferred to BG-11 liquid media. A homozygous culture was achieved by increasing kanamycin concentrations (50 μg/ml final concentration). Complete knock-out was confirmed via Southern blot analysis.
Construction of the Complemented Strain
Nucleotide sequence of orf222 was amplified from Guillardia theta DNA without its putative transit peptide by using the primers 222komp2_f (5'-CAT ATG AAT TAA AAC CAA TCC TTA ATT G -3') and 222komp_r (5'-GTT AAA ATT AAA TGA ATT CTA ATA A-3'). Two pairs of primers were used to amplify the flanking regions: 1649a_f (5'-GGT TAC TGC TCG AGG CGC ATC A-3') and 1649kompa1_r (5'-CAA TAA CTA CAT ATG TCC CAT TCC-3') generated the fragment Compa, which includes the upstream region for slr1649, 1649kompa2_f (5'-TTT ATG TCG AAT TCC ACT GAT C-3') and 1649b_r (5'- GAG ATT GGC CGC GGC AAT TTC TG-3') generated fragment Compb. All three fragments were ligated into the pGEM-T vector (Promega, Mannheim) and after verification of the sequence, transferred into the pBluescript II SK (Stratagene, Amsterdam) vector using different restrictions sides inserted by the primers leading to a precursor construct. By using EcoRI restriction sites, a spectinomycin cassette was cloned between the two fragments Compa/orf222 and Compb resulting in plasmid pComp222 Δslr1649. After transforming the Synechocystis sp. PCC 6803 wild type strain with this plasmid, transformants were selected on BG-11 agar plates supplemented with spectinomycin (5 μg/ml starting concentration). Spectinomycin resistant clones were transferred to BG-11 liquid media. A homozygous culture was achieved by increasing spectinomycin concentrations (30 μg/ml final concentration).
Nucleic Acid Analysis
Synechocystis sp. PCC 6803 cells were collected by centrifugation of 5 ml cell culture at 3200 × g. For DNA isolation, the pellet was resuspended in 400 μl TE buffer pH 7.0. After addition of breaking buffer (10% sodium dodecyl sulfat (w/v), 5% sodium lauryl sulfat (w/v)), 200 μl glass beads (0.2 mm diameter) and 400 μl phenol, cells were lysed by vortexing the suspension three times for 10 s. The suspension was then centrifuged at 12 000 × g and the resulting upper phase transferred to a new cup. This sample was treated twice with phenol-chloroform-isoamylalcohol (25:24:1) and centrifuged as before. By adding 1/10 Vol. NaAc pH 4.8 and two Vol. 96% ethanol, the DNA was precipitated for 1 h at -20°C. Afterwards, an additional washing step with 70% ethanol was performed. The pellet was dried and resuspended in H2O.
RNA was isolated from Synechocystis cells with Trizol© (Invitrogen, Karlsruhe) according to the manufacturer protocol. Northern Blot and Southern Blot analysis were performed according to standard protocols (Sambrook). Probes were constructed using the PCR DIG Probe Synthesis Kit (Roche, Mannheim).
Antibody Generation and Purification
To generate an antibody against Slr1649 we used the primers ex1649_f (5'-GGA TCC TTA TGT CCC ATT CCA CTG-3') and ex1649_r (5'-CTC GAG GCT GGC TAA AAA CTA ACT-3') to amplify the slr1649 gene, which was finally cloned in the pGEX-5X-3 vector (GE Healthcare Biosciences). After overexpression and purification of the Slr1649 GST fusion protein, immunization steps were executed by the Eurogentec company (Seraing).
The IgG fraction was purified from serum by protein A sepharose beads (GE Healthcare Biosciences).
Isolation of Phycobilisomes
Phycobilisome isolation was performed according to Gray et al. . Cells were collected by centrifugation at 5000 rpm for 10 min at room temperature. After an additional washing step with BG-11 media, cells were resuspended in 0.75 M potassium-phosphate buffer pH 7.0 (PPB), containing a protease inhibitors cocktail (PIC, 2 mg/ml Antipain, 5 mg/ml Chymostatin, 2 mg/ml Aprotinin, 5 mg/ml Trypsin-Inhibitor, 2 mg/ml Pepstatin, 5 mg/ml Leupeptin, 1 mg/ml Elastatinal and 2 mg/ml Na2EDTA in HEPES/KOH. Final concentration 200 μg/ml Inhibitor) and afterwards broken by two passes through a French press (Aminco) at 124 MPa. The lysates were incubated with Triton X-100 (2%) for 15 min at room temperature and subsequently centrifuged at 20 000 rpm for 1 h to pellet unbroken cells and membrane debris. The supernatant was immediately loaded on a 10%–40% linear sucrose gradient, solved in PPB and centrifuged at 18 000 rpm for 16 h at 15°C.
Standard SDS-PAGE was performed with an Hoefer SE 250 apparatus (83 mm × 101 mm, 0,75 mm thick) or a custom made system (250 mm × 150 mm and 1,0 mm thick) using the Laemmli buffer system . The polyacrylamide content in the separating gel was a gradient of 10% to 15%. The stacking gel contained 6% polyacrylamide. To achieve a better resolution of polypeptides with masses less than 15 kDa, the SDS-Tricine gel system was used . Staining of gels was generally carried out with Coomassie brilliant blue G-250 dissolved in solution A (2% phosphoric acid v/v, 10% (NH4)2SO4 w/v) and methanol (40:9:1). To visualize the bilin carrying proteins gels were incubated in a 0.2 M ZnSO4 solution [38, 39] and highlighted with UV in a transilluminator (Bio-Rad).
Formic Acid Cleavage
Phycobilisomes were precipitated with Methanol/Chloroform  and resuspended in cleavage buffer. Cleavage was done according to Piszkiewicz et al. . 30 μg of phycobilisomes were incubated for 16 h at 37°C with 70% formic acid in before adding SDS sample buffer and analysis by Tricine SDS-PAGE on a 17% polyacrylamide gel.
Isolation of Protein Extracts from Synechocystissp. PCC 6803
Synechocystis sp. PCC 6803 cells were grown and harvested as described above. The cell pellet was resuspended in TEN100 buffer , containing PIC. Cell lysis was performed as described above.
MALDI-TOF MS Analysis
The protein spots were subjected to in-gel trypsin digestion before mass spectrometry analysis as described previously . The peptide mixtures from the tryptic digests were desalted and concentrated using ZipTips™ columns made from the reverse chromatography resins Poros and Oligo R3 (Applied Biosystems). The bound peptides were washed with a solution of 0.5% formic acid and eluted from the column in 1 μl of 33% (v/v) acetonitrile/0.1% trifluoroacetic acid solution saturated with α-cyano-4-hydroxycinnamic (Bruker Daltonics) directly onto a MALDI target plate and air dried before analysis in the mass spectrometer. Mass spectrometry measurement was performed on an Ultraflex-TOF TOF tandem mass spectrometer (Bruker Daltonics). Peptide mass fingerprint spectra were acquired in the reflectron positive mode with a pulsed extraction using approximately 100 laser shots. The spectra were acquired after an external calibration using reference peptides (Peptide mixture II Bruker Daltonics). The acquired spectra were further internally calibrated using trypsin autolysis peaks as internal standards (842.5100, 2211.1046 Da). Monoisotopic masses were assigned and processed using Biotools ™ and FlexAnalysis ™ software (Bruker Daltonics) before submitting them to the Mascot program  for searches against the non-redundant NCBI database. The parameters used in the Mascot peptide mass fingerprint searches were as follows: Taxonomy, Synechocystis; search all molecular masses and all isoelectric points; allow up to one missed proteolytic cleavage site and a peptide mass tolerance of 50 ppm. Methionine oxidation was considered as an optional modification and cysteine carbamidomethylation as a fixed modification in all the searches. Matches to Synechocystis proteins were considered unambiguous when the probability score was significant using the Mascot score with a p value < 0.05 and when there was a minimum of five peptides and with a sequence coverage greater than 20%. For each protein the identity was further validated by tandem MS-MS analysis of selected peptides.
Blast search analyses were done by NCBI protein-protein blast  (see Additional file 1). This program was also used for conserved domain predictions. Transmembrane domains were predicted by using TMHMM server v. 2.0  and the SOSUI protein prediction server . Genome data from Synechocystis sp. PCC 6803 were obtained from CyanoBase . In silico cleavage was performed by PeptideMass .
The 41 sequences were aligned using MUSCLE  with 16 iterations. The output format was set to the standard ClustalW format . The alignment contained 307 sites including 191 gapped sites that were excluded from the analysis. From the remaining 116 sites logdet distances were estimated using LDDist 1.3.2 . These distances were used to construct a Neighbor-net splits graph  that was visualized with SplitsTree4 .
We thank Andrew Bozarth for comments on the manuscript. This work is supported by the Deutsche Forschungsgemeinschaft (SFB-TR1, TP A7).
- Bishop JE, Rapoport H, Klotz CV, Chan CF, Glazer AN, Füglistaller P, Zuber H: Chromopeptides from phycoerythrocyanin: structure and linkage of the three bilin groups. J Am Chem Soc. 1987, 109: 875-881. 10.1021/ja00237a039.View ArticleGoogle Scholar
- Stadnichuk I: Phycobiliproteins: Determination of chromophore composition and content. Phytochemical Analysis. 1995, 6: 281-288. 10.1002/pca.2800060602.View ArticleGoogle Scholar
- Holzwarth AR: Structure function relationships and energy transfer in phycobiliprotein antennae. Physiol Plant. 1991, 83: 518-528. 10.1111/j.1399-3054.1991.tb00129.x.View ArticleGoogle Scholar
- Fairchild CD, Zhao J, Zhou J, Colson SE, Bryant DA, Glazer AN: Phycocyanin alpha-subunit phycocyanobilin lyase. Proc Natl Acad Sci USA. 1992, 89: 7017-7021. 10.1073/pnas.89.15.7017.PubMedPubMed CentralView ArticleGoogle Scholar
- Fairchild CD, Glazer AN: Oligomeric structure, enzyme kinetics, and substrate specificity of the phycocyanin alpha subunit phycocyanobilin lyase. J Biol Chem. 1994, 269: 8686-8694.PubMedGoogle Scholar
- Jung LJ, Chan CF, Glazer AN: Candidate genes for the phycoerythrocyanin alpha subunit lyase. Biochemical analysis of pecE and pecF interposon mutants. J Biol Chem. 1995, 270: 12877-12884.PubMedView ArticleGoogle Scholar
- Zhao KH, Deng MG, Zheng M, Zhou M, Parbel A, Storf M, Meyer M, Strohmann B, Scheer H: Novel activity of a phycobiliprotein lyase: both the attachment of phycocyanobilin and the isomerization to phycoviolobilin are catalyzed by the proteins PecE and PecF encoded by the phycoerythrocyanin operon. FEBS Lett. 2000, 469: 9-13. 10.1016/S0014-5793(00)01245-X.PubMedView ArticleGoogle Scholar
- Storf M, Parbel A, Meyer M, Strohmann B, Scheer H, Deng MG, Zheng M, Zhou M, Zhao KH: Chromophore attachment to biliproteins: specificity of PecE/PecF, a lyase-isomerase for the photoactive 3(1)-cys-alpha 84-phycoviolobilin chromophore of phycoerythrocyanin. Biochemistry. 2001, 40: 12444-12456. 10.1021/bi010776s.PubMedView ArticleGoogle Scholar
- Zhao KH, Su P, Li J, Tu JM, Zhou M, Bubenzer C, Scheer H: Chromophore attachment to phycobiliprotein beta-subunits: phycocyanobilin:cysteine-beta84 phycobiliprotein lyase activity of CpeS-like protein from Anabaena Sp. PCC7120. J Biol Chem. 2006, 281: 8573-8581. 10.1074/jbc.M513796200.PubMedView ArticleGoogle Scholar
- Shen G, Saunee NA, Williams SR, Gallo EF, Schluchter WM, Bryant DA: Identification and characterization of a new class of bilin lyase: the cpcT gene encodes a bilin lyase responsible for attachment of phycocyanobilin to Cys-153 on the beta-subunit of phycocyanin in Synechococcus sp. PCC 7002. J Biol Chem. 2006, 281: 17768-17778. 10.1074/jbc.M602563200.PubMedView ArticleGoogle Scholar
- Zhao KH, Su P, Tu JM, Wang X, Liu H, Ploscher M, Eichacker L, Yang B, Zhou M, Scheer H: Phycobilin:cystein-84 biliprotein lyase, a near-universal lyase for cysteine-84-binding sites in cyanobacterial phycobiliproteins. Proc Natl Acad Sci USA. 2007, 104: 14300-14305. 10.1073/pnas.0706209104.PubMedPubMed CentralView ArticleGoogle Scholar
- Zhao KH, Zhang J, Tu JM, Bohm S, Ploscher M, Eichacker L, Bubenzer C, Scheer H, Wang X, Zhou M: Lyase activities of CpcS- and CpcT-like proteins from Nostoc PCC7120 and sequential reconstitution of binding sites of phycoerythrocyanin and phycocyanin beta-subunits. J Biol Chem. 2007, 282: 34093-34103. 10.1074/jbc.M703038200.PubMedView ArticleGoogle Scholar
- Glazer AN: Light guides. Directional energy transfer in a photosynthetic antenna. J Biol Chem. 1989, 264: 1-4.PubMedGoogle Scholar
- Apt KE, Collier JL, Grossman AR: Evolution of the phycobiliproteins. J Mol Biol. 1995, 248: 79-96. 10.1006/jmbi.1995.0203.PubMedView ArticleGoogle Scholar
- MacColl R: Cyanobacterial phycobilisomes. J Struct Biol. 1998, 124: 311-334. 10.1006/jsbi.1998.4062.PubMedView ArticleGoogle Scholar
- Bryant DA, Glazer AN, Eiserling FA: Characterization and structural properties of the major biliproteins of Anabaena sp. Arch Microbiol. 1976, 110: 61-75. 10.1007/BF00416970.PubMedView ArticleGoogle Scholar
- Glazer AN: Phycobiliproteins. Methods Enzymol. 1988, 167: 291-303.PubMedView ArticleGoogle Scholar
- de Marsac NT, Cohen-Bazire G: Molecular composition of cyanobacterial phycobilisomes. Proc Natl Acad Sci USA. 1977, 74: 1635-1639. 10.1073/pnas.74.4.1635.PubMedPubMed CentralView ArticleGoogle Scholar
- Lundell DJ, Williams RC, Glazer AN: Molecular architecture of a light-harvesting antenna. In vitro assembly of the rod substructures of Synechococcus 6301 phycobilisomes. J Biol Chem. 1981, 256: 3580-3592.PubMedGoogle Scholar
- Liu LN, Chen XL, Zhang YZ, Zhou BC: Characterization, structure and function of linker polypeptides in phycobilisomes of cyanobacteria and red algae: an overview. Biochim Biophys Acta. 2005, 1708: 133-142. 10.1016/j.bbabio.2005.04.001.PubMedView ArticleGoogle Scholar
- Glazer AN: Light harvesting by phycobilisomes. Annu Rev Biophys Biophys Chem. 1985, 14: 47-77. 10.1146/annurev.bb.14.060185.000403.PubMedView ArticleGoogle Scholar
- Douglas SE, Penny SL: The plastid genome of the cryptophyte alga, Guillardia theta: complete sequence and conserved synteny groups confirm its common ancestry with red algae. J Mol Evol. 1999, 48: 236-244. 10.1007/PL00006462.PubMedView ArticleGoogle Scholar
- Gould SB, Fan E, Hempel F, Maier UG, Klosgen RB: Translocation of a phycoerythrin alpha subunit across five biological membranes. J Biol Chem. 2007, 282: 30295-30302. 10.1074/jbc.M701869200.PubMedView ArticleGoogle Scholar
- Matsuzaki M, Misumi O, Shin IT, Maruyama S, Takahara M, Miyagishima SY, Mori T, Nishida K, Yagisawa F, Yoshida Y, Nishimura Y, Nakao S, Kobayashi T, Momoyama Y, Higashiyama T, Minoda A, Sano M, Nomoto H, Oishi K, Hayashi H, Ohta F, Nishizaka S, Haga S, Miura S, Morishita T, Kabeya Y, Terasawa K, Suzuki Y, Ishii Y, Asakawa S, Takano H, Ohta N, Kuroiwa H, Tanaka K, Shimizu N, Sugano S, Sato N, Nozaki H, Ogasawara N, Kohara Y, Kuroiwa T: Genome sequence of the ultrasmall unicellular red alga Cyanidioschyzon merolae 10D. Nature. 2004, 428: 653-657. 10.1038/nature02398.PubMedView ArticleGoogle Scholar
- Asano T, Yoshioka Y, Kurei S, Sakamoto W, Machida Y: A mutation of the CRUMPLED LEAF gene that encodes a protein localized in the outer envelope membrane of plastids affects the pattern of cell division, cell differentiation, and plastid division in Arabidopsis. Plant J. 2004, 38: 448-459. 10.1111/j.1365-313X.2004.02057.x.PubMedView ArticleGoogle Scholar
- Mann NH, Clokie MR, Millard A, Cook A, Wilson WH, Wheatley PJ, Letarov A, Krisch HM: The genome of S-PM2, a "photosynthetic" T4-type bacteriophage that infects marine Synechococcus strains. J Bacteriol. 2005, 187: 3188-3200. 10.1128/JB.187.9.3188-3200.2005.PubMedPubMed CentralView ArticleGoogle Scholar
- Cavalier-Smith T: Nucleomorphs: enslaved algal nuclei. Curr Opin Microbiol. 2002, 5: 612-619. 10.1016/S1369-5274(02)00373-9.PubMedView ArticleGoogle Scholar
- Maier UG, Douglas SE, Cavalier-Smith T: The nucleo morph genomes of cryptophytes and chlorarachniophytes. Protist. 2000, 151: 103-109. 10.1078/1434-4610-00011.PubMedView ArticleGoogle Scholar
- Douglas S, Zauner S, Fraunholz M, Beaton M, Penny S, Deng LT, Wu X, Reith M, Cavalier-Smith T, Maier UG: The highly reduced genome of an enslaved algal nucleus. Nature. 2001, 410: 1091-1096. 10.1038/35074092.PubMedView ArticleGoogle Scholar
- Federspiel NA, Grossman AR: Characterization of the light-regulated operon encoding the phycoerythrin-associated linker proteins from the cyanobacterium Fremyella diplosiphon. J Bacteriol. 1990, 172: 4072-4081.PubMedPubMed CentralGoogle Scholar
- Federspiel NA, Scott L: Characterization of a light-regulated gene encoding a new phycoerythrin-associated linker protein from the cyanobacterium Fremyella diplosiphon. J Bacteriol. 1992, 174: 5994-5998.PubMedPubMed CentralGoogle Scholar
- de Lorimier R, Bryant DA, Stevens SE: Genetic analysis of a 9 kDa phycocyanin-associated linker polypeptide. Biochim Biophys Acta. 1990, 1019: 29-41. 10.1016/0005-2728(90)90121-J.PubMedView ArticleGoogle Scholar
- Ughy B, Ajlani G: Phycobilisome rod mutants in Synechocystis sp. strain PCC6803. Microbiology. 2004, 150: 4147-4156. 10.1099/mic.0.27498-0.PubMedView ArticleGoogle Scholar
- Rippka R, Deruelles J, Waterbury JB, Herdman M, Stanier RY: Generic assignments, strain histories and properties of pure cultures of cyanobacteria. J Gen Microbiol. 1979, 111: 1-61.Google Scholar
- Gray BH, Lipschultz CA, Gantt E: Phycobilisomes from a blue-green alga Nostoc species. J Bacteriol. 1973, 116: 471-478.PubMedPubMed CentralGoogle Scholar
- Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970, 227: 680-685. 10.1038/227680a0.PubMedView ArticleGoogle Scholar
- Schagger H, von Jagow G: Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal Biochem. 1987, 166: 368-379. 10.1016/0003-2697(87)90587-2.PubMedView ArticleGoogle Scholar
- Berkelman TR, Lagarias JC: Visulalization of bilin-linked peptides and proteins in polyacrylamide gels. Anal Biochem. 1986, 156: 194-201. 10.1016/0003-2697(86)90173-9.PubMedView ArticleGoogle Scholar
- Zolla L, Bianchetti M: High-performance liquid chromatography coupled on-line with electrospray ionization mass spectrometry for the simultaneous separation and identification of the Synechocystis PCC 6803 phycobilisome proteins. J Chromatogr A. 2001, 912: 269-279. 10.1016/S0021-9673(01)00532-5.PubMedView ArticleGoogle Scholar
- Wessel D, Flugge UI: method for the quantitative recovery of protein in dilute solution in the presence of detergents and lipids. Anal Biochem. 1984, 138: 141-143. 10.1016/0003-2697(84)90782-6.PubMedView ArticleGoogle Scholar
- Piszkiewicz D, Landon M, Smith EL: Anomalous cleavage of aspartyl-proline peptide bonds during amino acid sequence determinations. Biochem Biophys Res Commun. 1970, 40: 1173-1178. 10.1016/0006-291X(70)90918-6.PubMedView ArticleGoogle Scholar
- Frangioni JV, Neel BG: Solubilization and purification of enzymatically active glutathione S-transferase (pGEX) fusion proteins. Anal Biochem. 1993, 210: 179-187. 10.1006/abio.1993.1170.PubMedView ArticleGoogle Scholar
- Hellmann U, Wernstedt C, Gonez J, Heldin CH: Improvement of an "In-Gel" digestion procedure for the micropreparation of internal protein fragments for amino acid sequencing. Anal Biochem. 1995, 224: 451-455. 10.1006/abio.1995.1070.View ArticleGoogle Scholar
- Mascot Program. [http://www.matrixscience.com]
- NCBI protein-protein blast. [http://www.ncbi.nlm.nih.gov/BLAST]
- TMHMM server v. 2.0. [http://www.cbs.dtu.dk/services/TMHMM-2.0]
- SOSUI protein prediction server. [http://bp.nuap.nagoya-u.ac.jp/sosui/]
- CyanoBase. [http://bacteria.kazusa.or.jp/cyanobase]
- PeptideMass. [http://www.expasy.ch/tools/peptide-mass.html]
- Edgar RC: MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res. 2004, 32: 1792-1797. 10.1093/nar/gkh340.PubMedPubMed CentralView ArticleGoogle Scholar
- Thompson JD, Higgins DG, Gibson TJ: CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 1994, 22: 4673-4680. 10.1093/nar/22.22.4673.PubMedPubMed CentralView ArticleGoogle Scholar
- Thollesson M: LDDist: a Perl module for calculating LogDet pair-wise distances for protein and nucleotide sequences. Bioinformatics. 2004, 20: 416-418. 10.1093/bioinformatics/btg422.PubMedView ArticleGoogle Scholar
- Bryant D, Moulton V: Neighbor-net: an agglomerative method for the construction of phylogenetic networks. Mol Biol Evol. 2004, 21: 255-265. 10.1093/molbev/msh018.PubMedView ArticleGoogle Scholar
- Huson DH, Bryant D: Application of phylogenetic networks in evolutionary studies. Mol Biol Evol. 2006, 23: 254-267. 10.1093/molbev/msj030.PubMedView ArticleGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.