LysoPC acyltransferase/PC transacylase activities in plant plasma membrane and plasma membrane-associated endoplasmic reticulum
© Larsson et al; licensee BioMed Central Ltd. 2007
Received: 23 March 2007
Accepted: 28 November 2007
Published: 28 November 2007
The phospholipids of the plant plasma membrane are synthesized in the endoplasmic reticulum (ER). The majority of these lipids reach the plasma membrane independently of the secretory vesicular pathway. Phospholipid delivery to the mitochondria and chloroplasts of plant cells also bypasses the secretory pathway and here it has been proposed that lysophospholipids are transported at contact sites between specific regions of the ER and the respective organelle, followed by lysophospholipid acylation in the target organelle. To test the hypothesis that a corresponding mechanism operates to transport phospholipids to the plasma membrane outside the secretory pathway, we investigated whether lysolipid acylation occurs also in the plant plasma membrane and whether this membrane, like the chloroplasts and mitochondria, is in close contact with the ER.
The plant plasma membrane readily incorporated the acyl chain of acyl-CoA into phospholipids. Oleic acid was preferred over palmitic acid as substrate and acyl incorporation occurred predominantly into phosphatidylcholine (PC). Phospholipase A2 stimulated the reaction, as did exogenous lysoPC when administered in above critical micellar concentrations. AgNO3 was inhibitory. The lysophospholipid acylation reaction was higher in a membrane fraction that could be washed off the isolated plasma membranes after repeated freezing and thawing cycles in a medium with lowered pH. This fraction exhibited several ER-like characteristics. When plasma membranes isolated from transgenic Arabidopsis expressing green fluorescent protein in the ER lumen were observed by confocal microscopy, membranes of ER origin were associated with the isolated plasma membranes.
We conclude that a lysoPC acylation activity is associated with plant plasma membranes and cannot exclude a PC transacylase activity. It is highly plausible that the enzyme(s) resides in a fraction of the ER, closely associated with the plasma membrane, or in both. We suggest that this fraction might be the equivalent of the mitochondria associated membrane of ER origin that delivers phospholipids to the mitochondria, and to the recently isolated ER-derived membrane fraction that is in close contact with chloroplasts. The in situ function of the lysoPC acylation/PC transacylase activity is unknown, but involvement in lipid delivery from the ER to the plasma membrane is suggested.
The composition of the lipid phase of plant plasma membranes adjusts to the varying conditions in the plant environment. The adjustments include selective lipid degradation, increased incorporation of certain lipid classes and/or lipid molecular species and possibly re-tailoring of the lipids within the membrane as well [1–5]. In addition to their structural role, plasma membrane lipids are crucial intermediates in several signaling pathways .
De novo synthesis of plasma membrane phospholipids occurs mainly in the endoplasmic reticulum (ER) [7–9]. The major plasma membrane phospholipids, phosphatidylcholine (PC) and phosphatidylethanolamine (PE) with C16 and C18 acylation of the sn-1 and sn-2 positions of the glycerol backbone, respectively, have been reported to be transported to the plasma membrane independently of the vesicular secretory pathway [8, 9]. The nature of lipid transport to the plant plasma membrane outside this pathway remains to be established, but for yeast and/or animal cells, lipid transport has been demonstrated to occur at membrane contacts sites (MCSs) between for example ER and mitochondria and ER and trans-Golgi membranes [10, 11]. In yeast, a plasma membrane-associated ER region was isolated. The fraction was denoted PAM (plasma membrane associated membrane), and lipid synthesis was enriched compared to bulk ER, whereas transport of lipids remains to be demonstrated . MCSs between ER and plasma membranes have not been reported for plants, but a close proximity between these membranes has been visualized by freeze fracture microscopy of suspension-cultured sycamore cells  and by confocal microscopy of Arabidopsis transformed with fluorescent tags on specific ER membrane proteins .
Mitochondria and chloroplast PC are also of ER origin . Presently, the most favoured model for lipid delivery to the mitochondria is that of lipid delivery at contact zones between a specialized ER region, denoted MAMs (mitochondria associated membranes), and the mitochondria . Biochemical [16–19] as well as biophysical  evidence is emerging for corresponding zones of contact between chloroplasts and a special region of the ER, denoted PLAMs (plastid associated membranes). Mitochondria  and chloroplasts [16, 18, 19] isolated from plant tissue both possess highly active lysoPC acylation activities and it has been suggested that in both cases, lysoPC is the lipid transported from the closely associated ER to the respective organelle.
To investigate the possibilities that phospholipid delivery to the plant plasma membrane outside the secretory apparatus could involve acylation of transported lysophospholipid and that a region of the ER could be involved, analogous to the situation for mitochondria and chloroplasts, we examined lysophosholipid acylation in isolated plasma membrane and a putative PAM fraction. We also present evidence for a PAM fraction in association with the plasma membrane.
Plasma membrane lipid acylation
The remaining acyl-CoA radiolabel was recovered in phospholipids, predominantly PC (Fig. 4B). The labelling was higher with [1-14C]18:1-CoA than with [1-14C]16:0-CoA as substrate, indicating a preference for acyl incorporation into the sn-2 position. After 30 min incubation with [1-14C]18:1-CoA, 62% of the phospholipid radiolabel was recovered in PC, whereas 27 and 11% was recovered in PE and phosphatidylinositol (PI), respectively (results not shown). With [1-14C]16:0-CoA, acyl incorporation was more evenly distributed between these three lipid classes: after 30 min incubation 39, 35 and 26% of the phospholipid radiolabel was recovered in PC, PE and PI, respectively (results not shown). Radiolabel was never recovered in phosphatidylglycerol. However, in a few experiments, 2–5% of the acyl-CoA radiolabel was recovered in phosphatidic acid (PA) (results not shown). When the radiolabelled acyl-CoA substrates were substituted with the corresponding radiolabelled free fatty acids, no radiolabelling of phospholipids was observed, demonstrating that the substrate for the acyltransferase was acyl-CoA (results not shown).
Acyl incorporation into PC and PE, comparing PAM with plasma membranes and an ER-rich fraction
Acyl incorporation from [14C]18:1-CoA (pmol·min-1·[mg protein]-1)
on its own
+lysoPC + AgNO3
PAM-decreased plasma membrane
on its own
+ lysoPC + AgNO3
on its own
+ lysoPC + AgNO3
on its own
+ lysoPC + AgNO3
Lipid acylation in PAM, a fraction of plasma membrane-associated membranes
The 10-step counter current membrane fractionation revealed that the ER marker choline phosphotransferase was active in fractions enriched in plasma membrane (Fig. 1A), indicating presence also of ER in these fractions. With isolated pea plasma membranes as starting material, we isolated a light membrane fraction, denoted PAM. We investigated acyl incorporation into PC and PE in the starting plasma membrane fraction, the PAM fraction, the PAM-free plasma membranes and the first, ER-rich, fraction from the 10-step counter current procedure. Acyl group incorporation from [1-14C]18:1-CoA into PC and PE verified the earlier results, that acyl incorporation into PC was faster in the ER-rich fraction than in the plasma membrane, whereas the reverse applied for acyl incorporation into PE (Table 1). Acyl incorporation into PC appeared somewhat sensitive to the procedure employed to fractionate the plasma membrane fraction, as this activity were lower in the PAM and PAM-free plasma membranes. Addition of lysoPC markedly stimulated acyl incorporation into PC in all fractions, with the highest rates in the ER-rich and PAM fractions. Here, the plasma membrane fraction was intermediate between the PAM and PAM-free plasma membrane fractions. The lysoPC acylation activity was sensitive to AgNO3, a known inhibitor of lysoPC acyl transferase , suggesting that a major part of the observed lysoPC acylation was indeed catalysed by this transferase.
Plasma membrane and PAM polypeptides
We separated the pea plasma membrane polypeptides by native gel electrophoresis and analyzed excised 1 mm sections for lysoPC acylation. The activity was present as a broad peak co-migrating with a broader major protein peak (results not shown). The fraction with the highest lysoPC acylation activity was submitted to tryptic digestion and linear ion trap mass spectrometry. The fraction contained a large number of polypeptides, but none related to lipid metabolism, probably due to the lack of annotation not only of pea peptides but also largely of lipid-related proteins.
Optical evidence for PAMs
Acylation of lysoPC has been demonstrated to occur in ER, chloroplasts and mitochondria, isolated from plant tissues. We here report that also plant plasma membranes catalyse the incorporation of acyl groups from acyl-CoA into phospholipids, with the dominant product being PC. The incorporation of the acyl group of acyl-CoA into plasma membrane phospholipids could have one or several roles: (i) to adjust the acylation pattern of plasma membrane phospholipids by trans-acylation as an involvement in the response to an altered external condition (stress), (ii) to acylate lysophopsholipids to turn off signalling systems that utilize PLA2, and/or (iii) to acylate lysophospholipids to provide the final step in supplying the plasma membrane with PC, analogous to that reported for mitochondria and chloroplasts, namely acylation of lysoPC of ER origin.
We clearly could demonstrate lysolipid acylation. The concentration of lysophospholipids in isolated plasma membranes has usually been reported to be very low [1–5], which fits well with their proposed signalling and regulatory roles. For example, lysoPC and lysoPE have been proposed to act as second messengers to auxin  and to be involved in systemic responses in wounded plants . LysoPC was recently reported to induce host plant genes that are involved in arbuscular mycorrhizal symbiosis . LysoPE and lysoPI have been shown to inhibit, but lysoPA to stimulate, plant phospholipase D . Lysolipids also affect membrane fusion . Evidently, the lysophospholipid content of the plasma membrane is strictly regulated. A lysophospholipid acylation activity may thus function to regulate signal-response cascades, enzyme activities or the fusibility of the plasma membrane.
Since 18:1-CoA was preferred over 16:0-CoA in the acylation reaction, the endogenous lysophospholipids probably had derived from PLA2-catalyzed phospholipid degradation. This conclusion is based on that plant plasma membrane phospholipids usually contain unsaturated C18 fatty acids in the sn-2 position, whereas 16:0 is usually restricted to the sn-1 position . The acylation activity increased when the content of lysophospholipids increased through addition of exogenous PLA2. The activity of PLA2 has been shown to suddenly increase at a threshold concentration of added lysoPC . This activation was thought to reflect an increased susceptibility of the membrane to PLA2. A similar change in membrane properties at a threshold concentration of lysophospholipid could be relevant also in the present case. However, the added lysoPC did not only stimulate the PLA2 activity, as incubations with radiolabelled lysoPC demonstrated their role as substrate for the acylation reaction.
When provided with exogenous lysophospholipid, the plasma membrane acylation activity had a markedly higher specificity for lysoPC than for other lysophospholipids. Larger than cmc concentrations of lysoPC was required to stimulate the reaction, which could reflect that the enzyme is activated in situ by a high local concentration of the substrate. The stimulatory effect of lysoPA on lysoPC acylation could indicate a regulatory role for lysoPA. The sensitivity to AgNO3 indicates that the activity is related to previously reported lysoPC acyl transferases.
Without added lysophospholipids, acyl group incorporation from acyl-CoA into phospholipids occurred with several phospholipid classes, whereas with exogenous substrate, only lysoPC was acylated. These results may suggest that more than one lysophospholipid acyl transferase is present in the plant plasma membrane. Another interpretation is that acyl group incorporation from acyl-CoA into phospholipids in the absence of added lysophospholipid represented a transacylase activity.
It has been demonstrated that PC and PE acylated with C16/C18 fatty acids are delivered to the plasma membrane independently of the vesicular secretory pathway [8, 9]. As it has been demonstrated that ER closely associates with the plasma membrane in yeast  and plant cells , we hypothesized that the delivery of lysoPC to the plant plasma membrane could occur at regions in close contact with the ER. Support for the hypothesis comes from several sources. In the 10-step counter-current separation of pea shoot membranes, the ER marker choline phosphotransferase to a minor extent also migrated with the plasma membrane marker (Fig. 1). In a previous 10-step separation of root membranes from phosphate-deficient oat , the ER marker NADPH cytochrome C reductase exhibited a dual distribution with the minor peak co-migrating with the plasma membrane. In an earlier report of a 5-step counter-current separation of microsomal membranes from cauliflower inflorescences , NADPH cytochrome C reductase and choline phosphotransferase activities co-localized in one major peak in the first tube as well as in a minor one co-migrating with the plasma membrane marker. In addition, we also observed that when plasma membranes were isolated from leaves of A. thaliana tagged with GFP in the ER lumen, small ER-derived structures co-isolated with the plasma membranes (Fig. 9). The association between the membranes was strong enough to survive the repeated freezing- and thawing procedure employed to invert a portion of the plasma membrane vesicles.
To isolate a putative membrane fraction of ER origin from the isolated plasma membrane, we modified the method developed for yeast PAMs . As aqueous polymer two-phase partition isolates cytoplasmic side-in plasma membrane vesicles , the putative PAM would be present inside the isolated plasma membrane vesicles. We therefore had to invert the plasma membrane vesicles prior to the yeast protocol treatment of lowering the pH to separate the PAMs from the plasma membrane . The protein patterns of the PAM and plasma membrane fractions were largely similar, although some proteins of the plasma membrane fraction were missing from the PAM fraction, whereas others were slightly enriched in the PAM fraction. Apparently, the PAM fraction was contaminated with plasma membrane. The freezing and thawing procedure employed to invert the plasma membrane vesicles prior to the PAM isolation probably resulted in the formation of small plasma membrane vesicles that co-isolated with the light PAM membrane vesicles. In addition, the freezing and thawing process could also have produced vesicles with surface properties intermediate between cytosolic side-out and cytosolic side-in plasma membrane vesicles, as we earlier reported for wheat plasma membranes . In the present case, we therefore cannot rule out that the isolation process could have produced vesicles of mixed plasma membrane and PAM origin.
In the PAM, addition of lysoPC caused a stronger increase in acyl group incorporation into PC compared with the original plasma membrane fraction, whereas in the PAM-decreased plasma membrane, the activity was much lower than in the original plasma membrane fraction. The differences may suggest different sets of acyl transferases or transacylases in the plasma membrane and its adjacent PAM. Another explanation could be that lysoPC acylation/PC transacylation actually resides in the ER regions associated with the plasma membrane, the PAM. If this is the case, the contamination of the PAM fraction with plasma membrane would have diluted the higher PAM-associated activity and as all PAM probably was not washed away from the plasma membrane, the difference in acylation/transacylation acitivies between the two fractions would have been underestimated. If lipid acylation/transacylation activities of isolated plasma membrane actually reflect PAM activities, such lipid metabolizing activities are not evenly distributed over the ER. A future extended characterization of PAM awaits development of an isolation protocol that increases fraction yield and purity.
We did not succeed in identifying any acyl transferase among the peptides present in the plasma membrane peptide fraction with the highest lysolipid acylation activity, which may reflect that these types of enzymes are not well characerized and therefore not annotated.
Our results demonstrate that isolated plant plasma membranes possess phospholipid acylation and/or transacylation activities. With endogenous substrate, the activity had a different lysophospholipid substrate preference than the corresponding activity of the ER. With added lysophospholipid substrate, both fractions were highly specific for lysoPC. We also present visual evidence for and the first tentative isolation of a plant PAM fraction, a membrane fraction of putative ER origin closely associated with the plasma membrane. The lysoPC acyl transferase activity was higher in this PAM fraction than in its parent plasma membrane fraction, whereas it was markedly lowered in the PAM-decreased plasma membrane. We propose that the zones of close contact between the ER and the plasma membrane, the PAMs, represents areas of the ER specialized in providing the plasma membrane with precursor for as well as synthesis of the PC that is delivered to the plasma membrane outside the secretory vesicular pathway. Whether both lysoPC acyl transferase and/or PC transacylase are constituents of the plasma membrane or the former or both activities are restricted to PAM is not yet resolved. The transport route for PE remains elusive.
Plant material and chemicals
Garden pea (Pisum sativum L., var Kelvedon Wonder) , soybean (Glycine max [L.] Merr., var. Folke)  and A. thaliana  were cultivated for 9–10 days, 4 days and 4–5 weeks, respectively, as described. The seeds of the transformed A. thaliana were kindly provided by Prof. I. Hara-Nishimura .
All chemicals and organic solvents were of analytical grade. Lipid standards, polyethylene glycol (PEG) and fine chemicals were obtained from Sigma (St. Louis, MO, USA) and organic solvents, TLC plates and inorganic salts from Merck (Darmstadt, Germany). Radiochemicals and Dextran T-500 were from Amersham Pharmacia Biotech (Uppsala, Sweden), and the fluorescent probe FM4–64 was from Invitrogen, Molecular Probes (Eugene, OR, USA).
Isolation of membrane fractions
Plasma membranes were isolated from microsomal fractions (filtered homogenate centrifuged for 10 min at 6 000 gmax, microsomes pelleted from for 30 min at 65 000 gmax) by aqueous polymer two-phase partitioning using one sample and two wash systems. The compositions of the systems were as previously optimized in the lab for pea shoots , soybean hypocotyls  and A. thaliana. The isolated plasma membranes were suspended in 0.25 M sucrose, 10 mM KCl, and 10 mM HEPES/KOH, pH 7.0, and used directly or frozen in liquid N2 prior to storage at -80°C.
To determine whether a membrane fraction of ER origin was associated with the plasma membranes, isolated pea shoot plasma membranes were suspended in 10 mM MES/KOH pH 6.0, and 0.33 M sucrose and subjected to three cycles of freezing in liquid nitrogen followed by thawing. As plasma membranes vesicles isolated by two phase partitioning are predominantly or exclusively oriented with the cytoplasmic surface inwards, this treatment was necessary to expose the cytoplasmic surface of a portion of the vesicles . A lowered pH was used as it had been required to separate PAMs from isolated yeast plasma membrane . After 10 min incubation at 4°C, the membrane suspension was top-loaded onto continuous sucrose gradient (20–50% w/v sucrose) in the same buffer. The gradient was centrifuged for 60 min in a swing out rotor at 100 000 × gmax. The band at the top of the gradient was collected as plasma membrane associated membranes (PAMs). These membranes and the resuspended plasma membrane pellet were diluted with 30 mM HEPES/KOH pH 7.0, 10 mM KCl and 2.5 mM MgCl2 and pelleted at 100 000 × gmax for 30 min.
The microsomes from pea shoots were also fractionated by aqueous polymer two-phase partition in a 10-step hand counter-current procedure . The 10 systems each weighed 10.00 g and contained 6.0% (w/w) Dextran T500, 6.0% (w/w) PEG3350, 5.0 mmol·kg-1 KPi, pH 7.8, and 0.25 mol·kg-1 sucrose. The membrane sample was included in the first tube. After shaking and phase separation, the upper phase of the first tube was transferred to a fresh lower phase in tube number two and fresh upper phase was added to the first tube. The procedure was repeated until the original upper phase had reached tube number 10 and all tubes contained both upper and lower phase. All 10 complete two-phase systems were each diluted with 10 mM HEPES/KOH pH 7.5, 0.25 M sucrose and 10 mM KCl and pelleted twice at 100,000 × gmax.
A membrane fraction enriched in ER was obtained from the first tube of the 10-step separation.
Acyl incorporation and lipid analysis
Suspended pea plasma membranes, containing 25 μg protein unless otherwise stated, were incubated with 26 μM [1-14C]18:1-CoA (2 Gbq/mmol) or [1-14C]16:0-CoA (2 Gbq/mmol) in a total volume of 100 μl of 0.33 M sucrose, 30 mM HEPES/pH 7.0, 10 mM KCl. With soybean plasma membranes, the assay routinely used 100 μg protein and 2.5 mM Mg-acetate was included in the assay. Further additions were according to figure and table legends. Prior to use, dissolved lysophospholipids were dried under nitrogen to remove the organic solvent, suspended in incubation medium and sonicated for 15 min. When [14C]lysoPC (sn-1 [14C]16:0, 2 Gbq/mmol) was used, 0.92 nmol was mixed with 7.2 nmol of unlabelled lysoPC (egg yolk; containing predominantly 16:0 or 18:0). The standard incubation time was 30 min and the reaction was stopped by adding 980 μl of a mixture of ice cold chloroform:methanol:water (30:60:15 by vol.). After addition of 0.5 ml 1.6 M HCl and 0.5 ml CHCl3, the lipids were extracted . An aliquot was removed for determination of total lipid radioactivity by liquid scintillation counting and the remainder was used for determination of radiolabel distribution between the lipids using thin layer chromatography and radio-scanning .
Other assays and experimental designs
The following marker enzymes were assayed: cytochrome C oxidase for mitochondrial inner membrane , choline phosphotransferase for endoplasmic reticulum , and 1,3-β-glucan synthase for plasma membrane . Chlorophyll was assayed for thylakoid membranes , and binding of a monoclonal anti-β-COP anti body, M3A5 (Sigma-Aldrich, St. Louis, US; previously validated for plants ), was assayed for Golgi membranes. Briefly, each fraction was dot-blotted onto a nitrocellulose membrane. Antibody incubation was performed as previously described  except for that M3A5 was used in a 1:200 dilution and the secondary antibody containing horseradish peroxidase conjugate (Pierce, Rockford, IL, USA) was diluted 1:4000. Chemoluminescence from the peroxidase reaction was detected on Hyper film ECL (GE-helthcare, Chalfont, UK) and intensities were quantified with Syngene Bio imaging system (Cambridge, UK). The protein concentration was measured using the bicinchoninic method  after solubilization of membrane fractions in 0.25% Triton X-100 for 10 min prior to addition of the protein determination reagent. SDS-PAGE, native gel electrophoresis and protein identification by MALDI-TOF or nano LC-FTISR spectroscopy (Swegene Proteomics Centre, Göteborg University, Sweden) were performed as described previously .
For the studies of the effects of PLA2, 0.001 U of bee venom PLA2 was used, together with 100 μM CaCl2. Exogenously added radiolabelled PC was sn-1,2 [14C]16:0-PC.
To observe plasma membrane-associated ER, we used A. thaliana transformed with GFP in the ER lumen . We chose ER lumen-localized GFP (instead of ER membrane-localized GFP) to maintain a native ER membrane surface for the studies of membrane-membrane interactions. The isolated plasma membrane fraction was subjected to cycles of freezing and thawing to invert a population of the vesicles . The resulting plasma membranes were visualized by using the fluorescent probe FM4-64. The confocal microscopy images were obtained as described  using equipment at the Centre for Cellular Imaging (Swegene, Göteborg University).
All experimental set ups were performed 2–5 times, each time using a plasma membrane fraction (or other membrane fraction) isolated from an independently cultivated plant batch. The data from one representative experiment from each set up are presented, as absolute radio-labelling varied between independent experiments. Trends or differences between treatments were consistent between independent experiments of the same design, except when noted.
0, hexadecanoic acid
0, octadecanoic acid
1, cis-9-octadecenoic acid
free fatty acid
green fluorescent protein
plasma membrane associated membrane
plastid associated membrane
Ms. Shirin Tavalley-Roodsari is acknowledged for the isolation of soybean plasma membranes. Financial support was from The Swedish Research Council for Agriculture, Environmental Sciences and Spatial Planning (Formas, to ASS), The Royal Swedish Academy of Agriculture and Forestry (to ASS) and Helge Ax:son Johnson Foundation (to JMK).
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