Proteomic analysis indicates massive changes in metabolism prior to the inhibition of growth and photosynthesis of grapevine (Vitis viniferaL.) in response to water deficit
© Cramer et al.; licensee BioMed Central Ltd. 2013
Received: 29 January 2013
Accepted: 14 March 2013
Published: 21 March 2013
Cabernet Sauvignon grapevines were exposed to a progressive, increasing water defict over 16 days. Shoot elongation and photosynthesis were measured for physiological responses to water deficit. The effect of water deficit over time on the abundance of individual proteins in growing shoot tips (including four immature leaves) was analyzed using nanoflow liquid chromatography - tandem mass spectrometry (nanoLC-MS/MS).
Water deficit progressively decreased shoot elongation, stomatal conductance and photosynthesis after Day 4; 2277 proteins were identified by shotgun proteomics with an average CV of 9% for the protein abundance of all proteins. There were 472 out of 942 (50%) proteins found in all samples that were significantly affected by water deficit. The 472 proteins were clustered into four groups: increased and decreased abundance of early- and late-responding protein profiles. Vines sensed the water deficit early, appearing to acclimate to stress, because the abundance of many proteins changed before decreases in shoot elongation, stomatal conductance and photosynthesis. Predominant functional categories of the early-responding proteins included photosynthesis, glycolysis, translation, antioxidant defense and growth-related categories (steroid metabolism and water transport), whereas additional proteins for late-responding proteins were largely involved with transport, photorespiration, antioxidants, amino acid and carbohydrate metabolism.
Proteomic responses to water deficit were dynamic with early, significant changes in abundance of proteins involved in translation, energy, antioxidant defense and steroid metabolism. The abundance of these proteins changed prior to any detectable decreases in shoot elongation, stomatal conductance or photosynthesis. Many of these early-responding proteins are known to be regulated by post-transcriptional modifications such as phosphorylation. The proteomics analysis indicates massive and substantial changes in plant metabolism that appear to funnel carbon and energy into antioxidant defenses in the very early stages of plant response to water deficit before any significant injury.
Technical and analytical advances over the last decade have dramatically changed our capabilities for gene discovery and functional genomics. Today there are many more transcriptomics analyses published than proteomics analyses of plants . Metabolites are the direct product of enzymatic reactions and the correlation of transcriptomics and proteomics profiles are generally 50% or lower, emphasizing the need for a greater focus on proteomics to fully understand plant growth and metabolism and their responses to environmental stress.
Ion toxicity and water deficit contribute to the inhibition of growth of most salt-stressed plants [2–4]. Osmotic shock experiments are typically performed to separate osmotic from ion toxicity effects [2, 3]. This type of experiment can provide useful information on the initial responses to sudden and severe stress [5–7], however, a gradual application of stress reveals a more complex plant response to the stress .
In an earlier study , we investigated the gradual effects of water deficit and salinity on the proteome of two grapevine cultivars, Chardonnay and Cabernet Sauvignon. Three time points (0, 8 and 16 days) were examined at the protein level only. Distinct differences in response to the stresses were found between the cultivars, but the time course lacked the resolution to discern early events in the responses of the vines to stress.
In order to gain deeper insights into the response of grapevine to water deficit and salinity, a larger number of time points were collected from a single cultivar, and transcript and metabolite profiles were assayed from aliquots of the same samples. Water deficit and salinity stress were applied gradually over a 16-day-period to pot-grown Cabernet Sauvignon vines in the greenhouse . Water was withheld over the course of the experiment for some plants and salt (NaCl:CaCl2 equal to 10:1) was applied gradually to other vines to effectively mimic the drop in stem water potential in the water-deficit-stressed vines. There were large and distinct differences in steady-state transcript abundance as measured by microarray analysis among the control, water-deficit and salt-stressed vines . Stress lowered stem water potentials equally in the stressed vines, but the growth of water-deficit-stressed vines was reduced more severely than salt-stressed vines. The inhibition of shoot elongation was detected 4 days after application of the stress treatments even though significant differences in stem water potentials were not detectable. The first significant differences in transcript abundance due to stress were detectable after 8 days of stress treatment when water potential began to decline significantly. Significant differences were found between the stress treatments in both the timing and the abundance of transcripts affected by the stress. Metabolite profiles of sugars, organic acids and amino acids were linked to transcript profiles revealing that photosynthesis, gluconeogenesis and photorespiration were affected by the stress treatments. Water-deficit-treated vines appeared to have greater demand for osmotic adjustment and detoxification of free radicals produced by photoinhibition than salt-stressed vines.
In the present proteomics study, a similar water deficit experiment was repeated to get additional details on the physiological and metabolic responses of Cabernet Sauvignon vines. One striking discovery from this analysis was that there were massive changes in protein abundance in a number of metabolic pathways prior to the inhibition of growth and photosynthesis. The early changes in protein abundance (e.g. increase in photosynthetic proteins and decrease in growth-related proteins) appear to be an acclimation response in anticipation of an upcoming water deficit. The later changes in protein abundance appear to be largely in response to significant stress injury (e.g. photorespiration).
The water deficit experiment was repeated in a similar manner as before  with 2-year-old Cabernet Sauvignon grapevines grown in pots in a greenhouse under nearly identical conditions. Control vines were watered daily with tap water, whereas water-deficit-treated vines were not irrigated. Shoot elongation, photosynthesis, leaf conductance and pot weight were monitored over the course of the 16-day experiment to determine how the stress progressed.
Proteins were extracted from the growing shoot tips (apex, stem, tendrils and four immature leaves) with a modified phenol-based protocol that is regularly used in the Cramer lab  and digested with trypsin in preparation for peptide analysis using nanoflow liquid chromatography-mass spectrometry (nanoLC-MS/MS). Peptide spectra were analyzed and quantified using both open source and custom-based bioinformatics software (see Methods for details). Protein identifications were filtered and false discovery rates (FDRs) calculated. Protein quantity was estimated as normalized spectral abundance factors (NSAF; see Methods). Approximately 27,000 spectra per sample were assigned to peptides matching 2277 Vitis vinifera proteins in the UniProtKB database (Additional file 1). The average CV for all 2277 proteins was approximately 9%.
Gene ontology (GO) categories of the identified proteins were downloaded from the UniProt database (uniprot.org) and assessed in BinGO (Additional file 2). There were hundreds of categories (850) with 240 significantly over-represented GO categories compared to the whole proteome at UniProt after FDR correction. Thus, there was wide representation of different classes of proteins in this dataset. The major categories included protein metabolic process, translation, organic acid metabolism, sugar metabolism, photosynthesis and protein folding.
The proteins were placed into absent (0) or present (1) categories for each treatment (Additional file 1). If any treatment had a protein missing in at least one of its replicates it was designated as absent. For example, 942 of the 2277 proteins were present in all replicates in all treatments (Additional file 1, key = 11111111 for Control Day 4, 8, 12, 16, Water Deficit Day 4, 8, 12, 16, respectively). Twenty-three proteins were present in the control vines, but missing altogether from the nonirrigated vines in at least one of the replicates for each day of the water deficit treatment (key = 11110000); another 125 proteins were missing in nonirrigated vines on Day 16 (key = 11111110) and 69 proteins were missing in nonirrigated vines on Day 12 and 16 (key = 11111100). Another interesting group of 20 proteins was consistently present on Day 12 and 16 of the water deficit treatment (key = 00000011). Some of these proteins may be missing because their average abundance was near the threshold for detection and were simply missing from a sample due to random variation. In other cases (e.g. key = 11111110; 00000011), some of the proteins in this category had substantial spectral counts and then disappeared or appeared on the specific day (e.g. Day 12 or 16 of the water deficit treatment).
Cluster 1 consisted of 159 proteins that increased significantly on Day 4 and remained elevated for the remainder of the water deficit treatment. A large number of GO biological process functional categories were overrepresented in this cluster (Additional file 4). The most significant category was photosynthesis based upon the p-value, followed by photorespiration and oxidation reduction. Other categories of interest included hexose metabolism, chromosome organization and oxidative stress (antioxidant) responses.
Cluster 4 consisted of 203 proteins that progressively decreased in abundance during the stress and were primarily involved in protein metabolism (translation and folding) and amino acid metabolism (Additional file 7). However, there were many other processes affected, such as gene expression, carbohydrate and phenylpropanoid metabolism. Furthermore, 34 of the 125 missing proteins on Day 16 (key = 11111110) whose average spectral counts were substantially higher than zero matched this decreasing profile (see Additional File 1). Most of these proteins missing on Day 16 of the water deficit treatment fit into the general C metabolism category.
Photosynthesis and photorespiration
The abundance of proteins involved in photosynthesis and photorespiration were strongly affected by water deficit (Figure 7). The abundance of many photosynthesis proteins were increased by water deficit on Day 4, whereas proteins involved in photorespiration were increased in abundance on Day 12. One of the most strongly affected proteins was the ferredoxin-NADP reductase (EC 220.127.116.11) located on the thylakoid membranes at the end of the Photosystem I (PS I) electron transport chain. The abundance of this protein fitted into Cluster 1 and was increased by water deficit by Day 4 and remained elevated compared to its control throughout the duration of the water deficit experiment. Othe proteins directly interacting or closely interacting with ferredoxin were also highly increased by water deficit including thioredoxin-f (Trx-f), superoxide dismutase (18.104.22.168), catalase (22.214.171.124), and peroxiredoxin (126.96.36.199). Glyceraldehyde-3-phosphate dehydrogenase (188.8.131.52) and sedoheptulose 1,7-bisphosphatase (184.108.40.206) of the Calvin-Benson cycle also had strong responses to water deficit. The abundance of most of the other photosynthetic proteins in the Calvin-Benson cycle were increased by water deficit on Day 4 but with varying patterns overtime (Figure 7). The abundance of the photorespiration proteins, glycolate oxidase (220.127.116.11), glycine dehydrogenase (18.104.22.168) and serine-glyoxylate aminotransferase (22.214.171.124) were increased on Day 12 by water deficit (Cluster 3). However, two isozymes of serine hydroxymethyltransferase (126.96.36.199) were increased on Day 4: D7T5C1 in Cluster 1 and F6GZK4 in Cluster 3; one isozyme, F6GWF3, was decreased on Day 12 (Cluster 4).
The increase of five key enzymes of the Calvin-Benson cycle indicates regulation by the ferredoxin-thioredoxin system . Consistent with this, the abundance of a thioredoxin-f type protein (Trx-f, D7WPN1, additional file 1) was significantly increased throughout the water deficit treatment (Figure 7). Note that this protein was not included in the 942 protein dataset because peptides were absent from one of the replicates of the control, Day 16 treatment. Nevertheless the response was statistically significant. Trx-f can regulate the enzymes of the Calvin-Benson cycle . Trx-f is also known to activate peroxiredoxins (188.8.131.52), which were highly increased throughout water deficit as well (Figure 7). Thus, some of the earliest and largest responses to water deficit appear to be localized around the activities of ferredoxin at the end of the electron transport chain of PS I. The activities are increased prior to any detectable decreases in physiological responses (growth and photosynthesis).
Glycolysis is important for the production of energy and carbon skeletons of primary and secondary metabolites. Water deficit had significant effects on proteins involved in glycolysis (Figure 8). The abundance of a number of enyzmes were increased (hexokinase-1, glyceraldehye-3-phosphate dehydrogenase, triose phosphate isomerase, phosphoglycerate kinase, malate dehydrogenase and malic enzyme) or decreased (phosphoglycerate mutase, phosphoglucomutase, phosphofructokinase, and pyruvate kinase) on Day 4. There are alternate forms of glycolysis [20, 21, 31, 32] used in different physiological conditions. Pyruvate kinase plays a critical role in the regulation of glycolysis . The large decrease in pyruvate kinase is consistent with a stimulation of an alternate pathway of glycolysis through phosphoenolpyruvate carboxylase (PEPC), malate dehydrogenase and malic enzyme (Figure 8). This alternate pathway of glycolysis is thought to be more efficient when plants are ADP-limited during stress [20, 33]. In addition, PEPC is very important for the replenishment of metabolites to the tricarboxylic acid cycle (TCA) cycle that are used for biosynthetic processes [31, 33].
The TCA cycle and the biosynthesis of glutamate, threonine, glycine and cysteine
The TCA cycle is a central hub in plant metabolism, giving rise to many primary and secondary metabolites including several intermediates involved in amino acid biosynthesis and nitrogen assimilation . The protein abundance of most of the enzymes of the TCA cycle were increased by water deficit (Figure 9). Two keys steps for amino acid biosynthesis are the production of 2-oxoglutarate by isocitrate dehydrogenase and oxaloacetate by malate dehydrogenase giving rise to glutamate and aspartate, respectively. These enzymes are significantly increased by water deficit (Figure 9). Glutamate is an important intermediate for other amino acids, purines, glutathione, chlorophyll and catalase (Figure 9). Glutamate synthase (184.108.40.206; GOGAT) is a key step in nitrogen assimilation  and its abundance was very elevated by water deficit. Interestingly, GOGAT uses reduced ferredoxin from PS I (also having an elevated protein abundance as described above) to convert one oxoglutarate and glutamine to two glutamates. The important antioxidant, glutathione, is a tripeptide synthesized from glutamate, cysteine and glycine. The abundance of proteins involved in the biosynthesis of all three of these amino acids was increased by water deficit. The protein abundance of threonine synthase (220.127.116.11) was highly increased by water deficit on Day 4 only. Threonine is used in the synthesis of glycine and pyridoxine (Vitamin B6); pyridoxine can act as an antioxidant and can protect abiotically-stressed plants [35, 36]. Note that the protein abundance of homoserine kinase (18.104.22.168) in the threonine biosynthesis pathway was highly increased by water deficit throughout the stress period. The serine biosynthesis pathway from 3-phosphoglycerate (see 3-phosphoglycerate dehydrogenase; 22.214.171.124) was downregulated by Day 12 of the water deficit, but this reduction might be compensated by the increased protein abundance of serine hydroxymethyltransferase (126.96.36.199). Consistent with these proteomics results, water deficit increases the abundance of serine, glycine and glutamate in the shoot tips of grapevine . Interestingly, proteins involved in purine metabolism were decreased in abundance (Additional file 1), perhaps contributing to a decrease in consumption of glutamate for this pathway and increasing glutamate supply for other pathways.
Consistent with the increased abundance of proteins involved with the biosynthesis of glycine, glutamate and cysteine and the H2O2 scavengers, catalase and peroxiredoxins, the abundance of antioxidant defense proteins in the ascorbate-glutathione cycle were increased (Figure 10). Ascorbate peroxidase (188.8.131.52) was highly elevated at Day 4 and remained elevated compared to control there after. Glutathione peroxidase (184.108.40.206) was increased at Day 4 and fits into Cluster 3 with increasing elevation of abundance over time (except on Day 8). Both of these enzymes scavenge H2O2. Longer term effects at Day 12 appear to contribute to longer term production of ascorbate by increasing galactose metabolism to produce ascorbate and decreasing tartrate biosynthesis to decrease ascorbate catabolism (Figure 10). The protein abundance of a UDP-glucose epimerase (220.127.116.11), which may be involved in the biosynthesis of galactose was increased and idonate dehydrogenase (18.104.22.1684), a key step in ascorbate conversion to tartrate , was decreased by water deficit.
Proteins involved in translation were decreased in abundance by water deficit (Cluster 2 and 4). There was a decrease of the protein abundance of six ribosomal proteins (A5ASC2, A5BUA6, A5BIA1, A5BHY1, F6HTT2, and F6GXM7) and one argynyl-tRNA synthetase (F6HGX8) on Day 4. These proteins are involved in translation and precede the detectable inhibition of shoot elongation by water deficit on Day 6 (see Cluster 2 proteins in Additional file 5 and Figure 4). The abundance of additional proteins involved in translation were identified in Cluster 4 (Additional file 7). These include 19 ribosomal proteins, 6 tRNA synthetases, 5 elongation factors, 3 subunits of eukaryotic translation initiation factor 3, a nascent polypeptide-associated complex (NAC), an alpha subunit family protein, and the GCN1 translational activator.
Phenylalanine is an important precursor of phenylpropanoids. The abundance of proteins involved in the shikimate pathway and the biosynthesis of phenylalanine were significantly decreased by water deficit (Figure 11). Phospho-2-dehydro-3-deoxyheptonate aldolase (22.214.171.124) is the first critical step for the shikimate pathway and phenylpropanoid biosynthesis  and its abundance was decreased significantly on Day 4 and throughout the water deficit (Figure 11). Phenylpropanoids are used in lignin biosynthesis, which represents a significant pathway (sink) for C and energy flow . This pathway may be decreased to conserve C skeletons and energy for other pathways. Consistent with this hypothesis, lignin content was found to be decreased in drought-stressed maize leaves . In addition, the abundance of chalcone synthase was decreased more than any other protein by water deficit (Additional file 1). Chalcone synthase is one of the key proteins regulating the biosynthesis of flavonoids, a subclass of phenylpropanoids that are involved in defense responses .
Summary of proteomic responses
Proteomic analyses in this study indicated that the proteome was dynamic, changing early in response to water deficit. More than 200 protein profiles changed prior to any detectable changes in growth or photosynthesis in response to water deficit.
Inhibition of growth
The inhibition of shoot or cell elongation is one of the most sensitive responses to water deficit and generally precedes the inhibition of photosynthesis [41–47]; however, in this study we could not distinguish the difference between the initial start in the decrease of growth and photosynthesis; growth and photosynthesis appeared equally sensitive to water deficit. Unlike osmotic shock treatments, the gradual application of stress in this experiment enabled the gradual progression of events to be evaluated. In three previous reports [8, 9, 47], shoot elongation was shown to be very sensitive to water deficit, decreasing before decreases in stem water potential were statistically detectable. Water-deficit at equivalent water potentials has a greater initial impact than salinity on shoot elongation of grapevines .
Interestingly, there was an early decrease in abundance of the brassinosteroid biosynthesis protein, Diminuto/DWF1(Day 4; Figure 5), preceding the inhibition of shoot elongation by water deficit (Day 6; Figure 1). This is a novel and intriguing observation. Knockouts of the Diminuto/DWF1 gene cause a dwarf phenotype in Arabidopsis. The Diminuto/DWF1 gene was cloned in grapes and shown to be associated with brassinosteroid biosynthesis in grape berries . It is not known if steroid concentrations change in response to water deficit in grapevine. Another protein that may affect growth was the decrease in protein abundance of an aquaporin (PIP1;4) on Day 4 that may regulate water uptake into the shoot tip.
Inhibition of translation
Like growth, protein synthesis is very sensitive to water deficit [41, 49–52], but it has been difficult to assess whether or not a decrease in growth preceded a decrease in translation or vice versa. In the present study there was a clear decrease of the protein abundance of multiple ribosomal proteins and an aminoacyl t-RNA synthase that are involved in translation on Day 4. Again, the decrease in protein abundance preceded the detectable inhibition of shoot elongation by water deficit on Day 6 (see Cluster 2 proteins in Additional file 5 and Figure 4).
Effects on photosynthesis
In the present study, the abundance of numerous photosynthesis-related proteins was increased (Additional file 4 and Figure 7) by Day 4 prior to the inhibition of photosynthesis (Figure 1). Note some caution in interpretation of these data is warranted, because proteins and photosynthesis were measured on different leaves. The proteins were extracted from the growing shoot tip which contained immature leaves that were largely acting as sinks, however some of these leaves of the growing shoot tip were most likely active in photosynthetic function and probably responded in a similar manner as the fully mature leaves. The photosynthesis measurements were measured on the first, fully-expanded leaf adjacent to the growing tip, acting as a source rather than a sink. Many of the identified photosynthetic proteins in the growing shoot tip function in light harvesting, carbon fixation, photosystem II, photosystem repair, and the regulation of light reactions.
The transcript profiles of most of the genes encoding these proteins in the shoot tip did not correlate with the protein profiles (unpublished results). In fact, there were no statistically significant changes induced by stress in any transcript profiles until Day 8 in a previous experiment , when growth is significantly inhibited and stem water potentials have significantly declined. The data indicate that the early changes in protein abundance of photosynthetic enzymes are involved in photosynthetic maintenance and repair and that no real impairment of photosynthesis is occurring at that time (an elastic strain rather than a plastic strain as defined by Levitt ). This argument is supported by a short-term stress experiment comparing chilling to osmotic stress in the shoot tips of Cabernet Sauvignon , which indicated that photosynthetic mechanisms could be repaired in a short-term osmotic stress, but repair mechanisms were inhibited in a short-term chilling stress.
Similar metabolic acclimation occurs in Arabidopsis . While there were no significant decreases in photosynthesis by mild water stress, because of the decreased growth there was an actual increase in carbon availability for the production of low molecular weight metabolites such as proline, organic acids (malate and fumarate) and hexoses. Furthermore, enzyme activities were elevated by water deficit to maintain photosynthesis and glycolysis .
Water stress inhibits photosynthesis in two stages . In the first stage, photosynthesis is primarily limited by CO2 diffusion. In the second stage, as the stress becomes more severe, metabolic inhibition occurs. Associated with this metabolic impairment is an increase in the activity of antioxidant mechanisms . In the present proteomics study, clear increases in antioxidant defenses were detected on Day 4, well before a decline in photosynthesis or growth. In a previous transcriptomics and metabolomics study , a clear increase in antioxidant mechanisms occurred starting at Day 12. In the transcript and metabolite profiles of the previous study, water-deficit-stressed plants were more strongly affected than salt-stressed plants. The data indicate that plants exposed to water-deficit are subject to more reactive oxygen species (ROS) and photoinhibition than salt-stressed plants. It appears that the increase in the metabolites, glucose, glutamate, glycine, proline and malate, and the large increase in transcripts involved in photorespiration and antioxidant proteins at Day 12 facilitate osmotic adjustment, ROS detoxification and protection against photoinhibition in these severely water-stressed plants. In particular, glutamate appears to be a central metabolite in this response as it is used in many metabolic pathways involved in the grapevine response to water deficit including amino acid metabolism (γ-amino-butyric acid (GABA), proline, glycine, etc.), nitrogen assimilation, and the biosynthesis of catalase, glutathione, and chlorophyll. Glutamate metabolic flux appears to drive GABA production more than proline in water-deficit stressed tobacco and GABA provides greater protection against ROS than proline .
Adjustments in glycolysis
Environmental stresses can cause subtle adjustments in glycolysis in plants [20, 45]. Two major functions of glycolysis are to produce energy and the building-blocks needed for biochemical synthesis. There are multiple ways that this pathway can be finely controlled including pH, substrate concentrations, metabolite effector concentrations and protein modification . Such adjustments in glycolysis have been observed with hypoxia and nutrient deprivation [20, 32, 33]. We could not find any studies on the effect of water deficit on an alternate pathway of glycolysis involving pyruvate kinase and PEPC. In arabidopsis leaves, steady-state water deficit increased the activity of pyruvate kinase and PEPC . Overall activities in the enzymes of the TCA cycle were maintained during water deficit, but alternate paths of glycolysis were not discussed. There was an increase in C supply in the plants, which was attributed to a reduction of growth before photosynthesis. Alternative oxidase in respiration is another alternate pathway affected by water deficit [58, 59]. The changes in an alternate glycolysis observed here in grapevine may allow energy to be used more efficiently  and may contribute to an increased flux of glutamate through various metabolic pathways.
Protein amount does not necessarily equate to protein activity. Many proteins are affected by post-translational modifications such as phosphorylation. A large number of key regulatory proteins in central C and amino acid metabolism have altered phosphorylation states depending on environmental conditions . Furthermore, there are significant changes in the phosphoproteome of growing maize leaves in response to water deficit . Many of the proteins detected in the present grapevine study are regulated by phosphorylation including hexokinase, phosphofructokinase, pyruvate kinase, PEPC, citrate synthase, threonine synthase, and 3-phosphoglycerate dehydrogenase to name but a few. Therefore additional investigations are necessary to determine the phosphorylation status of these enzymes and their activity to further clarify their role in metabolism during water deficit.
Comparisons to other plant proteomics studies of water deficit
There are quite a few studies on the proteomics response of plants to water deficit  most of which have been performed with 2-D gel electrophoresis. Several studies indicate an effect of water deficit on photosynthetic enzymes and C metabolism [8, 62–65], antioxidant defenses [66–71], and protein synthesis .
In grapevine, a proteomics analysis of water deficit and salinity on shoot tips  showed that the inhibition of shoot elongation was correlated with the inhibition of proteins involved in photosynthesis, protein synthesis and protein fate. Many of the enzymes affected by water deficit in the present study were not affected significantly by water deficit in the previous study. The lack of significance may be due to the relatively large CV found when using 2-D PAGE, which has an average CV of approximately 50% (unpublished results), wherease the CV in the present study using nanoLC-MS/MS was only 9%. The high CV makes it much more difficult to detect statistical significance even though the treatment effects may be real.
In drought-stressed grape berry skins , similar to the present study on shoot tips, long-term water deficit increased the abundance of ascorbate peroxidase. In the berry pulp, the abundance of glutamate decarboxylase was increased by water deficit. Glutamate decarboxylase was decreased by water deficit (Cluster 4) in shoot tips in the present study. The study on berry tissues indicates that there are differential responses to water deficit depending on the tissue; this is likely related to the function of the tissue or organ.
One proteomics study shows clear root signaling in rice in response to water deficit . A split-root design was used to analyze the effects of water deficit on both the well-watered and water-deficit treated roots. A number of proteins were up-regulated in the well-watered split roots of rice as compared to ordinary well-watered roots; that is the well-watered split roots were receiving signals from the water-deficit-treated split roots. One of the proteins up-regulated in the well-watered split roots was a universal stress protein and a number of proteins involved in ROS defense, including several in the ascorbate-glutathione cycle. This is very similar to the early responsive proteins of grapevine shoot tips in the present study.
There are significant changes in the xylem sap proteome of maize in response to water deficit . Proteins associated with cell wall metabolism and biotic defense response increased in abundance in response to water deficit after changes in stomatal conductance including cell wall peroxidases. Interestingly, several metabolites changed early in the xylem sap before decreasing stomatal conductance including ABA, malate and some amino acids.
In a very similar experimental design to the present study, the abundance of phosphoproteins in growing maize leaves was affected by mild and severe water deficit . One difference in the experimental design to the present study was that the first sampling occurred soon after growth inhibition, not before. In addition, the rate of dehydration was faster than the present study with mild water deficit occurring after 1 day and severe water deficit occurring after 4 days. In addition, a rehydration response was also investigated in the maize study. The phosphoproteomics response to water deficit and rehydration was very dynamic, affecting 132 out of 1235 reproducible phosphopeptides. The top three functional categories affected were chromosome remodeling, cell expansion and phytohormone signaling, respectively. Early stress responsive phosphoproteins were involved in cell division and cell wall biosynthesis. Upon rewatering, there were rapid responses in phosphoproteins involving ABA, ethylene and jasmonate signaling.
Speculation on root signals and feedforward mechanisms
The change in protein abundance prior to any detectable changes in physiology in response to water deficit in the present grapevine study may indicate a feedforward mechanism caused by hormone and/or hydraulic signals from the root [75–80]. Tall and narrow tree pots were used in this study. These pots would allow a water gradient to form in the pot; the upper roots would dry out first. Roots in the bottom of the pot, where most of the roots are located, would continue transporting water to the shoot tips to supply enough water for shoot elongation. The signal would have been produced in the root tips that were drying out in the upper portion of the pot.
ABA has been described as such a feedforward signal from drying root tips that prematurely closes stomates to reduce water loss from the soil in advance of water deficit [79, 80], however in the present study stomatal conductance decreased after changes in protein metabolism. A hydraulic signal due to a changing water potential gradient [77, 80, 81] is another possibility, however, this gradient would have to be small, because it did not significantly affect shoot elongation. A third possibility is a long-distance ROS signal that was elegantly demonstrated to respond rapidly to various biotic and abiotic stresses [82, 83]. It remains to be demonstrated if slow drying of root tips could trigger such a signal. It is possible that ferredoxin may be an early target of such ROS signaling because the abundance of a number of antioxidant proteins that interact with ferredoxin were some of the most highly increased by water deficit on Day 4. Future experiments with greater time resolution are needed to test these signaling hypotheses.
In summary, the analyses of protein profiles have provided a deeper insight into the response of water-deficit-stressed plants. In this study, the quantitative proteomic analysis of grapevine shoot tips exposed to water deficit indicated that stress affected many proteins involved in metabolism, energy (photosynthesis, glycolysis and respiration), antioxidant defense and protein fate (protein synthesis, folding and degradation). Many of these changes may represent acclimation responses, occurring prior to any detectable changes in shoot elongation and photosynthesis. Early changes in protein abundance in response to stress occurred with photosynthesis, translation-, growth- and C metabolism that might be affected by phosphorylation. Many other proteins are known to be regulated by ROS and ferredoxin-thioredoxin signaling. These early and massive proteomic responses to water deficit indicate that there was an increase in C metabolism and energy production, shifting away from growth and toward protective antioxidant defenses. Longer-term effects on protein abundance indicated that more severe water deficit impacted additional processes involving photorespiration, stress responses, protein fate and cellular defense.
Plant material and experimental conditions
Plant material and experimental conditions along with physiological assays have been described in detail in previous publications [5, 9]. Briefly, two-year-old rooted cuttings of dormant Vitis vinifera cv. Cabernet Sauvignon clone 8 were grown in 13.3 liter pots containing approximately 10 L SuperSoil® potting mix supplemented with slow release fertilizer. Plants were grown in a greenhouse on a 16 h light (26°C, minimum 400 μE m-2 s-1)/8 h dark (18°C) cycle supplemented with illumination from sodium vapor lamps. Vines were irrigated daily with tap water (control) or not irrigated at all (water deficit).
Growth was measured repeatedly on five independent biological replicates that were harvested 16 days after the start of the experiment. Elongating shoot tips of 3 individual biological replicates for each time point (Day 4, 8, 12, and 16) and treatment (control and water deficit) were harvested and frozen immediately in liquid nitrogen in preparation for protein extraction. Elongating shoot tips consisted of the shoot apex, stems, tendrils and the first four immature leaves. Photosynthesis and stomatal conductance were measured with a LI-6400XT portable photosynthesis system (LI-COR, Lincoln, NE, USA) on the first fully expanded leaf (leaf number 6 from the apex) adjacent to the shoot tip.
Proteins were extracted from frozen, finely-ground shoot tip samples using a phenol-based extraction protocol . In preparation for gel-free shotgun proteomics, protein pellets were Lys-C- and trypsin-digested using a modified method (A. Apffel, personal communication) of the Filter-Aided Sample Preparation (FASP) methods [84, 85]. The modified FASP uses trifluoroethanol (TFE) instead of urea to denature proteins. Protein extracts (250 μg each) were dissolved in 200 μL 50% TFE, 0.1 M ammonium bicarbonate, 50 mM dithiothreitol (DTT) and concentrated to ~20 μL in Amicon Ultra 0.5 mL 10K ultrafiltration devices (Millipore). Then 100 μL 50% TFE, 0.1 M ammonium bicarbonate (ABC), 50 mM iodoacetamide was added to the devices, incubated at room temperature 1 h, followed by centrifugation (14,000 g, 45 min). The alkylated proteins were then washed five times in the ultracentrifugation devices with 200 μL 50% TFE, 0.1 M ABC. To the ~20 μL retentates, 45 μL 50% TFE, 0.1 M ABC was added, then 2.5 μg Lys-C (Wako) from a 0.5 μg/μL stock solution. Following overnight incubation at 28°C, 350 μL 20% acetonitrile (CAN), 50 mM ABC was added to each digest, followed by 2.5 μL of 1 μg/μL modified trypsin (Promega). The trypsin digests took place at 37°C for two hours, then they were stopped with 10 μL 50% formic acid. The resulting peptides were passed through the ultrafiltration membranes by centrifugation (14,000 g, 45 min), followed by a 150 μL rinse of the UF devices with 50% ACN, 2% formic acid. The peptide filtrates were concentrated by Speedvac to near-dryness, then reconstituted in 12.5 μL 40% formic acid, 10% TFE and diluted for LC-MS/MS with 60 μL water.
LC-MS/MS spectra were acquired from 3 biological replicates per treatment and timepoint by a sample-optimized gas phase fractionation (GPF) method (similar to ) on an LTQ XL mass spectrometer (Thermo). Chromatography was performed on a Surveyor autosampler/LC system (Thermo) and 0.3 x 150 mm PLRP-S column (3 μm, 100 Å, Agilent) interfaced with the mass spectrometer by an Advance captive spray source (Michrom). Each sample (10 μL injections) was separated by four 180 min LC-MS/MS runs at 5 μL min-1 differing only in precursor m/z ranges. The m/z ranges for four gas phase fractions per sample were optimized empirically by analyzing a mixture of pooled samples from m/z 400–2000, then creating GPF fractions to approximately evenly distribute peptide observations among the four fractions. Using data dependent acquisition of MS/MS spectra of the most abundant six peaks per MS scan (with dynamic exclusion), the analysis time of 12 h per sample produced approximately 118,000 MS/MS spectra per sample.
A protein database was compiled from three sources 10 September 2011: 1) all reviewed V. vinifera protein entries in UniProt (151 sequences); 2) V. vinifera proteins predicted by the International Grape Genome Program (29749 sequences identified by UniProt search “Taxonomy:29760 AND author:vitulo AND reviewed:no”); 3) mitochondrial proteins associated in UniProt with  and  (81 non-redundant sequences).
Spectrum-peptide matching was performed with X!Tandem and the GPM Cyclone (http://www.thegpm.org) in automated mode using MudPit merging. Default ion trap parameters were used with the exceptions of MS error (+3, -1 Da), the inclusion of point mutations, the inclusion of reversed sequences, and a protein expect value of −1. Approximately 27,000 spectra per sample were assigned to peptides.
Normalized spectral abundance factors (NSAF) were calculated according to Zybailov et al. . Protein identifications were filtered and protein and peptide FDRs calculated according to Gammulla et al. . Protein identifications were excluded if they did not match at least one spectrum in all three biological replicates and at least a total of six spectra among the replicates. The protein false discovery rate was 0.57% and the peptide false discovery rate was 0.056%. The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium (http://proteomecentral.proteomexchange.org) via the PRIDE partner repository  with the dataset identifier PXD000123.
The authors would like to thank Supakan Rattanakon for her assistance in protein extraction and Alex Apffel for the modified FASP method (unpublished).
- Cramer GR, Urano K, Delrot S, Pezzotti M, Shinozaki K: Effects of abiotic stress on plants: a systems biology perspective. BMC Plant Biol. 2011, 11: 163. 10.1186/1471-2229-11-163.PubMedPubMed CentralView ArticleGoogle Scholar
- Munns R: Comparative physiology of salt and water stress. Plant Cell Environ. 2002, 25 (2): 239-250. 10.1046/j.0016-8025.2001.00808.x.PubMedView ArticleGoogle Scholar
- Greenway H, Munns R: Mechanisms of salt tolerance in nonhalophytes. Annu Rev Plant Physiol. 1980, 31: 149-190. 10.1146/annurev.pp.31.060180.001053.View ArticleGoogle Scholar
- Tester M, Davenport R: Na+ tolerance and Na+ transport in higher plants. Ann Bot. 2003, 91 (5): 503-527. 10.1093/aob/mcg058.PubMedPubMed CentralView ArticleGoogle Scholar
- Tattersall EA, Grimplet J, Deluc L, Wheatley MD, Vincent D, Osborne C, Ergul A, Lomen E, Blank RR, Schlauch KA, Cushman JC, Cramer GR: Transcript abundance profiles reveal larger and more complex responses of grapevine to chilling compared to osmotic and salinity stress. Funct Integr Genomics. 2007, 7 (4): 317-333. 10.1007/s10142-007-0051-x.PubMedView ArticleGoogle Scholar
- Kreps JA, Wu Y, Chang HS, Zhu T, Wang X, Harper JF: Transcriptome changes for Arabidopsis in response to salt, osmotic, and cold stress. Plant Physiol. 2002, 130 (4): 2129-2141. 10.1104/pp.008532.PubMedPubMed CentralView ArticleGoogle Scholar
- Seki M, Narusaka M, Ishida J, Nanjo T, Fujita M, Oono Y, Kamiya A, Nakajima M, Enju A, Sakurai T, Satou M, Akiyama K, Taji T, Yamaguchi-Shinozaki K, Carninci P, Kawai J, Hayashizaki Y, Shinozaki K: Monitoring the expression profiles of 7000 Arabidopsis genes under drought, cold and high-salinity stresses using a full-length cDNA microarray. Plant J. 2002, 31 (3): 279-292. 10.1046/j.1365-313X.2002.01359.x.PubMedView ArticleGoogle Scholar
- Vincent D, Ergul A, Bohlman MC, Tattersall EA, Tillett RL, Wheatley MD, Woolsey R, Quilici DR, Joets J, Schlauch K, Schooley DA, Cushman JC, Cramer GR: Proteomic analysis reveals differences between Vitis vinifera L. cv. Chardonnay and cv. Cabernet Sauvignon and their responses to water deficit and salinity. J Exp Bot. 2007, 58 (7): 1873-1892. 10.1093/jxb/erm012.PubMedView ArticleGoogle Scholar
- Cramer GR, Ergul A, Grimplet J, Tillett RL, Tattersall EA, Bohlman MC, Vincent D, Sonderegger J, Evans J, Osborne C, Quilici D, Schlauch KA, Schooley DA, Cushman JC: Water and salinity stress in grapevines: early and late changes in transcript and metabolite profiles. Funct Integr Genomics. 2007, 7 (2): 111-134. 10.1007/s10142-006-0039-y.PubMedView ArticleGoogle Scholar
- Vincent D, Wheatley MD, Cramer GR: Optimization of protein extraction and solubilization for mature grape berry clusters. Electrophoresis. 2006, 27 (9): 1853-1865. 10.1002/elps.200500698.PubMedView ArticleGoogle Scholar
- Saeed AI, Bhagabati NK, Braisted JC, Liang W, Sharov V, Howe EA, Li J, Thiagarajan M, White JA, Quackenbush J: TM4 microarray software suite. Methods Enzymol. 2006, 411: 134-193.PubMedView ArticleGoogle Scholar
- Lundin B, Hansson M, Schoefs B, Vener AV, Spetea C: The Arabidopsis PsbO2 protein regulates dephosphorylation and turnover of the photosystem II reaction centre D1 protein. Plant J. 2007, 49 (3): 528-539. 10.1111/j.1365-313X.2006.02976.x.PubMedView ArticleGoogle Scholar
- Taiz L, Zeiger E: Plant Physiology, 5th ed. SinauerAssociates, Inc: Sunderland, MA; 2010.Google Scholar
- Vandeleur RK, Mayo G, Shelden MC, Gilliham M, Kaiser BN, Tyerman SD: The role of PIP aquaporins in water transport through roots: diurnal and drought stress responses reveal different strategies between isohydric and anisohydric cultivars of grapevine. Plant Physiol. 2009, 149 (1): 445-460. 10.1104/pp.108.128645.PubMedPubMed CentralView ArticleGoogle Scholar
- Gambetta GA, Manuck CM, Drucker ST, Shaghasi T, Fort K, Matthews MA, Walker MA, McElrone AJ: The relationship between root hydraulics and scion vigour across Vitis rootstocks: what role do root aquaporins play?. J Exp Bot. 2012, 63 (18): 6445-6455. 10.1093/jxb/ers312.PubMedPubMed CentralView ArticleGoogle Scholar
- Klahre U, Noguchi T, Fujioka S, Takatsuto S, Yokota T, Nomura T, Yoshida S, Chua NH: The Arabidopsis DIMINUTO/DWARF1 gene encodes a protein involved in steroid synthesis. Plant Cell. 1998, 10 (10): 1677-1690.PubMedPubMed CentralView ArticleGoogle Scholar
- Jager CE, Symons GM, Ross JJ, Reid JB: Do brassinosteroids mediate the water stress response?. Physiol Plant. 2008, 133 (2): 417-425. 10.1111/j.1399-3054.2008.01057.x.PubMedView ArticleGoogle Scholar
- Oliver MJ, Jain R, Balbuena TS, Agrawal G, Gasulla F, Thelen JJ: Proteome analysis of leaves of the desiccation-tolerant grass, Sporobolus stapfianus, in response to dehydration. Phytochem. 2011, 72 (10): 1273-1284. 10.1016/j.phytochem.2010.10.020.View ArticleGoogle Scholar
- Carroll AJ, Heazlewood JL, Ito J, Millar AH: Analysis of the Arabidopsis cytosolic ribosome proteome provides detailed insights into its components and their post-translational modification. Mol Cell Proteomics. 2008, 7 (2): 347-369.PubMedView ArticleGoogle Scholar
- Plaxton WC: The organization and regulation of plant glycolysis. Annu Rev Plant Physiol Plant Mol Biol. 1996, 47: 185-214. 10.1146/annurev.arplant.47.1.185.PubMedView ArticleGoogle Scholar
- Sweetman C, Deluc LG, Cramer GR, Ford CM, Soole KL: Regulation of malate metabolism in grape berry and other developing fruits. Phytochem. 2009, 70 (11–12): 1329-1344.View ArticleGoogle Scholar
- Mhamdi A, Queval G, Chaouch S, Vanderauwera S, Van BF, Noctor G: Catalase function in plants: a focus on Arabidopsis mutants as stress-mimic models. J Exp Bot. 2010, 61 (15): 4197-4220. 10.1093/jxb/erq282.PubMedView ArticleGoogle Scholar
- Kang J, Hwang JU, Lee M, Kim YY, Assmann SM, Martinoia E, Lee Y: PDR-type ABC transporter mediates cellular uptake of the phytohormone abscisic acid. Proc Natl Acad Sci USA. 2010, 107 (5): 2355-2360. 10.1073/pnas.0909222107.PubMedPubMed CentralView ArticleGoogle Scholar
- Marton MJ, Crouch D, Hinnebusch AG: GCN1, a translational activator of GCN4 in Saccharomyces cerevisiae, is required for phosphorylation of eukaryotic translation initiation factor 2 by protein kinase GCN2. Mol Cell Biol. 1993, 13 (6): 3541-3556.PubMedPubMed CentralView ArticleGoogle Scholar
- Viotti C, Luoni L, Morandini P, De MMI: Characterization of the interaction between the plasma membrane H-ATPase of Arabidopsis thaliana and a novel interactor (PPI1). FEBS J. 2005, 272 (22): 5864-5871. 10.1111/j.1742-4658.2005.04985.x.PubMedView ArticleGoogle Scholar
- Ho CL, Noji M, Saito M, Saito K: Regulation of serine biosynthesis in Arabidopsis. Crucial role of plastidic 3-phosphoglycerate dehydrogenase in non-photosynthetic tissues. J Biol Chem. 1999, 274 (1): 397-402. 10.1074/jbc.274.1.397.PubMedView ArticleGoogle Scholar
- Zhang P, Foerster H, Tissier CP, Mueller L, Paley S, Karp PD, Rhee SY: MetaCyc and AraCyc. Metabolic pathway databases for plant research. Plant Physiol. 2005, 138 (1): 27-37. 10.1104/pp.105.060376.PubMedPubMed CentralView ArticleGoogle Scholar
- Ogata H, Goto S, Sato K, Fujibuchi W, Bono H, Kanehisa M: KEGG: Kyoto Encyclopedia of Genes and Genomes. Nucleic Acids Res. 1999, 27 (1): 29-34. 10.1093/nar/27.1.29.PubMedPubMed CentralView ArticleGoogle Scholar
- Grimplet J, Cramer GR, Dickerson JA, Mathiason K, Van Hemert J, Fennell AY: VitisNet: “Omics” integration through grapevine molecular networks. PLoS One. 2009, 4 (12): e8365. 10.1371/journal.pone.0008365.PubMedPubMed CentralView ArticleGoogle Scholar
- Buchanan BB, Balmer Y: Redox regulation: a broadening horizon. Annu Rev Plant Biol. 2005, 56: 187-220. 10.1146/annurev.arplant.56.032604.144246.PubMedView ArticleGoogle Scholar
- Tcherkez G, Boex-Fontvieille E, Mahe A, Hodges M: Respiratory carbon fluxes in leaves. Curr Opin Plant Biol. 2012, 15 (3): 308-314. 10.1016/j.pbi.2011.12.003.PubMedView ArticleGoogle Scholar
- van Dongen JT, Gupta KJ, Ramirez-Aguilar SJ, Araujo WL, Nunes-Nesi A, Fernie AR: Regulation of respiration in plants: a role for alternative metabolic pathways. J Plant Physiol. 2011, 168 (12): 1434-1443. 10.1016/j.jplph.2010.11.004.PubMedView ArticleGoogle Scholar
- O’Leary B, Park J, Plaxton WC: The remarkable diversity of plant PEPC (phosphoenolpyruvate carboxylase): recent insights into the physiological functions and post-translational controls of non-photosynthetic PEPCs. Biochem J. 2011, 436 (1): 15-34. 10.1042/BJ20110078.PubMedView ArticleGoogle Scholar
- Foyer CH, Noctor G, Hodges M: Respiration and nitrogen assimilation: targeting mitochondria-associated metabolism as a means to enhance nitrogen use efficiency. J Exp Bot. 2011, 62 (4): 1467-1482. 10.1093/jxb/erq453.PubMedView ArticleGoogle Scholar
- Havaux M, Ksas B, Szewczyk A, Rumeau D, Franck F, Caffarri S, Triantaphylides C: Vitamin B6 deficient plants display increased sensitivity to high light and photo-oxidative stress. BMC Plant Biol. 2009, 9: 130. 10.1186/1471-2229-9-130.PubMedPubMed CentralView ArticleGoogle Scholar
- Chen H, Xiong L: Pyridoxine is required for post-embryonic root development and tolerance to osmotic and oxidative stresses. Plant J. 2005, 44 (3): 396-408. 10.1111/j.1365-313X.2005.02538.x.PubMedView ArticleGoogle Scholar
- DeBolt S, Cook DR, Ford CM: L-tartaric acid synthesis from vitamin C in higher plants. Proc Natl Acad Sci USA. 2006, 103: 5608-5613. 10.1073/pnas.0510864103.PubMedPubMed CentralView ArticleGoogle Scholar
- Novaes E, Kirst M, Chiang V, Winter-Sederoff H, Sederoff R: Lignin and biomass: a negative correlation for wood formation and lignin content in trees. Plant Physiol. 2010, 154 (2): 555-561. 10.1104/pp.110.161281.PubMedPubMed CentralView ArticleGoogle Scholar
- Vincent D, Lapierre C, Pollet B, Cornic G, Negroni L, Zivy M: Water deficits affect caffeate O-methyltransferase, lignification, and related enzymes in maize leaves. A proteomic investigation. Plant Physiol. 2005, 137 (3): 949-960. 10.1104/pp.104.050815.PubMedPubMed CentralView ArticleGoogle Scholar
- Dao TT, Linthorst HJ, Verpoorte R: Chalcone synthase and its functions in plant resistance. Phytochem Rev. 2011, 10 (3): 397-412. 10.1007/s11101-011-9211-7.PubMedPubMed CentralView ArticleGoogle Scholar
- Bradford KJ, Hsiao TC: Physiological responses to moderate water stress. Physiological Plant Ecology II, Volume 12 B. Edited by: Lange OL, Nobel PS, Osmond CB, Ziegler H. New York Berlin Heidelberg: Springer-Verlag; 1982: 263-324.View ArticleGoogle Scholar
- Cramer GR, Alberico GJ, Schmidt C: Leaf expansion limits dry matter accumulation of salt-stressed maize. Aust J Plant Physiol. 1994, 21: 663-674. 10.1071/PP9940663.View ArticleGoogle Scholar
- Yeo AR, Lee KS, Izard P, Boursier PJ, Flowers TJ: Short- and long-term effects of salinity on leaf growth in rice (Oryza sativa L.). J Exp Bot. 1991, 42 (240): 881-889.View ArticleGoogle Scholar
- Saab IN, Sharp RE: Non-hydraulic signals from maize roots in drying soil: inhibition of leaf elongation but not stomatal conductance. Planta. 1989, 179 (4): 466-474. 10.1007/BF00397586.PubMedView ArticleGoogle Scholar
- Hummel I, Pantin F, Sulpice R, Piques M, Rolland G, Dauzat M, Christophe A, Pervent M, Bouteille M, Stitt M, Gibon Y, Muller B: Arabidopsis plants acclimate to water deficit at low cost through changes of carbon usage: an integrated perspective using growth, metabolite, enzyme, and gene expression analysis. Plant Physiol. 2010, 154 (1): 357-372. 10.1104/pp.110.157008.PubMedPubMed CentralView ArticleGoogle Scholar
- Tardieu F, Granier C, Muller B: Water deficit and growth. Co-ordinating processes without an orchestrator. Curr Opin Plant Biol. 2011, 14 (3): 283-289. 10.1016/j.pbi.2011.02.002.PubMedView ArticleGoogle Scholar
- Bonhomme L, Valot BÆ, Tardieu F, Zivy M: Phosphoproteome Dynamics Upon Changes in Plant Water Status Reveal Early Events Associated With Rapid Growth Adjustment in Maize Leaves. Mol Cell Proteomics. 2012, 11 (10): 957-972. 10.1074/mcp.M111.015867.PubMedPubMed CentralView ArticleGoogle Scholar
- Symons GM, Davies C, Shavrukov Y, Dry IB, Reid JB, Thomas MR: Grapes on steroids. Brassinosteroids are involved in grape berry ripening. Plant Physiol. 2006, 140: 150-158.PubMedPubMed CentralView ArticleGoogle Scholar
- Ben-Zioni A, Itai C, Vaadia Y: Water and salt stresses, kinetin and protein synthesis in tobacco leaves. Plant Physiol. 1967, 42: 361-365. 10.1104/pp.42.3.361.PubMedPubMed CentralView ArticleGoogle Scholar
- Flowers TJ, Dalmond D: Protein synthesis in halophytes: the influence of potassium, sodium and magnesium in vitro. Plant Soil. 1992, 146: 153-161. 10.1007/BF00012008.View ArticleGoogle Scholar
- Dhindsa RS, Cleland RE: Water stress and protein synthesis: I. Differential inhibition of protein synthesis. Plant Physiol. 1975, 55 (4): 778-781. 10.1104/pp.55.4.778.PubMedPubMed CentralView ArticleGoogle Scholar
- Hsiao TC: Rapid Changes in Levels of Polyribosomes in Zea mays in Response to Water Stress. Plant Physiol. 1970, 46 (2): 281-285. 10.1104/pp.46.2.281.PubMedPubMed CentralView ArticleGoogle Scholar
- Levitt J: Responses of plants to environmental stresses. Volume 1. Chilling freezing, and high temperature stresses. New York: Academic Press; 1980.Google Scholar
- Ben Jouira H, Schlauch KA, Cushman JC, Cramer GR: Short-term abiotic stresses effects on proteomic profiles of Cabernet Sauvignon shoot tips. BMC Plant Biol. 2012, submittedGoogle Scholar
- Flexas J, Bota J, Galmes J, Medrano H, Ribas-Carbo M: Keeping a positive carbon balance under adverse conditions: responses of photosynthesis and respiration to water stress. Physiol Plant. 2006, 127 (3): 343-352. 10.1111/j.1399-3054.2006.00621.x.View ArticleGoogle Scholar
- Cho K, Shibato J, Agrawal GK, Jung YH, Kubo A, Jwa NS, Tamogami S, Satoh K, Kikuchi S, Higashi T, Kimura S, Saji H, Tanaka Y, Iwahashi H, Masuo Y, Rakwal R: Integrated transcriptomics, proteomics, and metabolomics analyses to survey ozone responses in the leaves of rice seedling. J Proteome Res. 2008, 7 (7): 2980-2998. 10.1021/pr800128q.PubMedView ArticleGoogle Scholar
- Liu C, Zhao L, Yu G: The dominant glutamic acid metabolic flux to produce gamma-amino butyric acid over proline in Nicotiana tabacum leaves under water stress relates to its significant role in antioxidant activity. J Integr Plant Biol. 2011, 53 (8): 608-618. 10.1111/j.1744-7909.2011.01049.x.PubMedView ArticleGoogle Scholar
- Rasmusson AG, Fernie AR, Van DJT: Alternative oxidase: a defence against metabolic fluctuations. Physiol Plant. 2009, 137 (4): 371-382. 10.1111/j.1399-3054.2009.01252.x.PubMedView ArticleGoogle Scholar
- Vanlerberghe GC, Cvetkovska M, Wang J: Is the maintenance of homeostatic mitochondrial signaling during stress a physiological role for alternative oxidase?. Physiol Plant. 2009, 137 (4): 392-406. 10.1111/j.1399-3054.2009.01254.x.PubMedView ArticleGoogle Scholar
- Oliveira AP, Ludwig C, Picotti P, Kogadeeva M, Aebersold R, Sauer U: Regulation of yeast central metabolism by enzyme phosphorylation. Mol Syst Biol. 2012, 8: 623.PubMedPubMed CentralView ArticleGoogle Scholar
- Kosova K, Vitamvas P, Prasil IT, Renaut J: Plant proteome changes under abiotic stress - Contribution of proteomics studies to understanding plant stress response. J Proteomics. 2011, 74 (8): 1301-1322. 10.1016/j.jprot.2011.02.006.PubMedView ArticleGoogle Scholar
- Bonhomme L, Monclus R, Vincent D, Carpin S, Lomenech AM, Plomion C, Brignolas F, Morabito D: Leaf proteome analysis of eight Populus xeuramericana genotypes: genetic variation in drought response and in water-use efficiency involves photosynthesis-related proteins. Proteomics. 2009, 9 (17): 4121-4142. 10.1002/pmic.200900047.PubMedView ArticleGoogle Scholar
- Kausar R, Arshad M, Shahzad A, Komatsu S: Proteomics analysis of sensitive and tolerant barley genotypes under drought stress. Amino Acids. 2012, 44 (2): 345-359.PubMedView ArticleGoogle Scholar
- Shu L, Lou Q, Ma C, Ding W, Zhou J, Wu J, Feng F, Lu X, Luo L, Xu G, Mei H: Genetic, proteomic and metabolic analysis of the regulation of energy storage in rice seedlings in response to drought. Proteomics. 2011, 11 (21): 4122-4138. 10.1002/pmic.201000485.PubMedView ArticleGoogle Scholar
- Bazargani MM, Sarhadi E, Bushehri AA, Matros A, Mock HP, Naghavi MR, Hajihoseini V, Mardi M, Hajirezaei MR, Moradi F, Ehdaie B, Salekdeh GH: A proteomics view on the role of drought-induced senescence and oxidative stress defense in enhanced stem reserves remobilization in wheat. J Proteomics. 2011, 74 (10): 1959-1973. 10.1016/j.jprot.2011.05.015.PubMedView ArticleGoogle Scholar
- Hajheidari M, Eivazi A, Buchanan BB, Wong JH, Majidi I, Salekdeh GH: Proteomics uncovers a role for redox in drought tolerance in wheat. J Proteome Res. 2007, 6 (4): 1451-1460. 10.1021/pr060570j.PubMedView ArticleGoogle Scholar
- Hajheidari M, Abdollahian-Noghabi M, Askari H, Heidari M, Sadeghian SY, Ober ES, Hosseini Salekdeh G: Proteome analysis of sugar beet leaves under drought stress. Proteomics. 2005, 5 (4): 950-960. 10.1002/pmic.200401101.PubMedView ArticleGoogle Scholar
- Sengupta D, Kannan M, Reddy AR: A root proteomics-based insight reveals dynamic regulation of root proteins under progressive drought stress and recovery in Vigna radiata (L.) Wilczek. Planta. 2011, 233 (6): 1111-1127. 10.1007/s00425-011-1365-4.PubMedView ArticleGoogle Scholar
- Wendelboe-Nelson C, Morris PC: Proteins linked to drought tolerance revealed by DIGE analysis of drought resistant and susceptible barley varieties. Proteomics. 2012, 12 (22): 3374-3385. 10.1002/pmic.201200154.PubMedView ArticleGoogle Scholar
- Benesova M, Hola D, Fischer L, Jedelsky PL, Hnilicka F, Wilhelmova N, Rothova O, Kocova M, Prochazkova D, Honnerova J, Fridrichova L, Hnilickova H: The physiology and proteomics of drought tolerance in maize: early stomatal closure as a cause of lower tolerance to short-term dehydration?. PLoS One. 2012, 7 (6): e38017. 10.1371/journal.pone.0038017.PubMedPubMed CentralView ArticleGoogle Scholar
- Mirzaei M, Soltani N, Sarhadi E, Pascovici D, Keighley T, Salekdeh GH, Haynes PA, Atwell BJ: Shotgun Proteomic Analysis of Long-distance Drought Signaling in Rice Roots. J Proteome Res. 2012, 11 (1): 348-358. 10.1021/pr2008779.PubMedView ArticleGoogle Scholar
- Mohammadi PP, Moieni A, Hiraga S, Komatsu S: Organ-specific proteomic analysis of drought-stressed soybean seedlings. J Proteomics. 2012, 75 (6): 1906-1923. 10.1016/j.jprot.2011.12.041.PubMedView ArticleGoogle Scholar
- Grimplet J, Wheatley MD, Jouira HB, Deluc LG, Cramer GR, Cushman JC: Proteomic and selected metabolite analysis of grape berry tissues under well watered and water-deficit stress conditions. Proteomics. 2009, 9: 2503-2528. 10.1002/pmic.200800158.PubMedPubMed CentralView ArticleGoogle Scholar
- Alvarez S, Marsh EL, Schroeder SG, Schachtman DP: Metabolomic and proteomic changes in the xylem sap of maize under drought. Plant Cell Environ. 2008, 31 (3): 325-340. 10.1111/j.1365-3040.2007.01770.x.PubMedView ArticleGoogle Scholar
- Perez-Alfocea F, Ghanem ME, Gomez-Cadenas A, Dodd IC: Omics of root-to-shoot signaling under salt stress and water deficit. Omics. 2011, 15 (12): 893-901. 10.1089/omi.2011.0092.PubMedView ArticleGoogle Scholar
- Davies WJ, Tardieu F, Trejo CL: How do chemical signals work in plants that grow in drying soil. Plant Physiol. 1994, 104 (2): 309-314.PubMedPubMed CentralGoogle Scholar
- Tardieu F, Davies WJ: Integration of hydraulic and chemical signalling in the control of stomatal conductance and water status of droughted plants. Plant Cell Environ. 1993, 16 (4): 341-349. 10.1111/j.1365-3040.1993.tb00880.x.View ArticleGoogle Scholar
- Schachtman DP, Goodger JQ: Chemical root to shoot signaling under drought. Trends Plant Sci. 2008, 13 (6): 281-287. 10.1016/j.tplants.2008.04.003.PubMedView ArticleGoogle Scholar
- Davies W, Kudoyarova G, Hartung W: Long-distance ABA Signaling and Its Relation to Other Signaling Pathways in the Detection of Soil Drying and the Mediation of the Plant‚Âôs Response to Drought. J Plant Growth Regul. 2005, 24 (4): 285-295. 10.1007/s00344-005-0103-1.View ArticleGoogle Scholar
- Tardieu F, Parent B, Simonneau T: Control of leaf growth by abscisic acid: hydraulic or non-hydraulic processes?. Plant Cell Environ. 2010, 33 (4): 636-647. 10.1111/j.1365-3040.2009.02091.x.PubMedView ArticleGoogle Scholar
- Tang AC, Boyer JS: Growth-induced water potentials and the growth of maize leaves. J Exp Bot. 2002, 53 (368): 489-503. 10.1093/jexbot/53.368.489.PubMedView ArticleGoogle Scholar
- Mittler R, Vanderauwera S, Suzuki N, Miller G, Tognetti VB, Vandepoele K, Gollery M, Shulaev V, Van BF: ROS signaling: the new wave?. Trends Plant Sci. 2011, 16 (6): 300-309. 10.1016/j.tplants.2011.03.007.PubMedView ArticleGoogle Scholar
- Miller G, Schlauch K, Tam R, Cortes D, Torres MA, Shulaev V, Dangl JL, Mittler R: The plant NADPH oxidase RBOHD mediates rapid systemic signaling in response to diverse stimuli. Sci Signal. 2009, 2 (84): ra45. 10.1126/scisignal.2000448.PubMedView ArticleGoogle Scholar
- Manza LL, Stamer SL, Ham AJ, Codreanu SG, Liebler DC: Sample preparation and digestion for proteomic analyses using spin filters. Proteomics. 2005, 5 (7): 1742-1745. 10.1002/pmic.200401063.PubMedView ArticleGoogle Scholar
- Wisniewski JR, Zougman A, Nagaraj N, Mann M: Universal sample preparation method for proteome analysis. Nat Methods. 2009, 6 (5): 359-362. 10.1038/nmeth.1322.PubMedView ArticleGoogle Scholar
- Scherl A, Shaffer SA, Taylor GK, Kulasekara HD, Miller SI, Goodlett DR: Genome-specific gas-phase fractionation strategy for improved shotgun proteomic profiling of proteotypic peptides. Anal Chem. 2008, 80 (4): 1182-1191. 10.1021/ac701680f.PubMedView ArticleGoogle Scholar
- Picardi E, Horner DS, Chiara M, Schiavon R, Valle G, Pesole G: Large-scale detection and analysis of RNA editing in grape mtDNA by RNA deep-sequencing. Nucleic Acids Res. 2010, 38 (14): 4755-4767. 10.1093/nar/gkq202.PubMedPubMed CentralView ArticleGoogle Scholar
- Goremykin VV, Salamini F, Velasco R, Viola R: Mitochondrial DNA of Vitis vinifera and the issue of rampant horizontal gene transfer. Mol Biol Evol. 2009, 26 (1): 99-110.PubMedView ArticleGoogle Scholar
- Zybailov B, Mosley AL, Sardiu ME, Coleman MK, Florens L, Washburn MP: Statistical analysis of membrane proteome expression changes in Saccharomyces cerevisiae. J Proteome Res. 2006, 5 (9): 2339-2347. 10.1021/pr060161n.PubMedView ArticleGoogle Scholar
- Gammulla CG, Pascovici D, Atwell BJ, Haynes PA: Differential metabolic response of cultured rice (Oryza sativa) cells exposed to high- and low-temperature stress. Proteomics. 2010, 10 (16): 3001-3019. 10.1002/pmic.201000054.PubMedView ArticleGoogle Scholar
- Vizcaino JA, Cote R, Reisinger F, Barsnes H, Foster JM, Rameseder J, Hermjakob H, Martens L: The Proteomics Identifications database: 2010 update. Nucleic Acids Res. 2010, 38 (Database issue): D736-D742.PubMedPubMed CentralView ArticleGoogle Scholar