Figure 9From: Agrobacterium-mediated genetic transformation of Coffea arabica (L.) is greatly enhanced by using established embryogenic callus culturesSouthern blot analyses of transgenic coffee plants. Coffee DNA from leaf material was digested with EcoRI, electrophoresed and probed with a 32P labeled HPTII gene fragment. The number of bands reflects the number of transgene insertion sites. Lane 1, untransformed coffee (control). Lanes 2-12, transgenic plants from independent transformation events. P - plasmid control (pMDC32). The size of the markers is indicated on the left.Back to article page