Identification, functional characterization and developmental regulation of sesquiterpene synthases from sunflower capitate glandular trichomes

Background Sesquiterpene lactones are characteristic metabolites of Asteraceae (or Compositae) which often display potent bioactivities and are sequestered in specialized organs such as laticifers, resin ducts, and trichomes. For characterization of sunflower sesquiterpene synthases we employed a simple method to isolate pure trichomes from anther appendages which facilitated the identification of these genes and investigation of their enzymatic functions and expression patterns during trichome development. Results Glandular trichomes of sunflower (Helianthus annuus L.) were isolated, and their RNA was extracted to investigate the initial steps of sesquiterpene lactone biosynthesis. Reverse transcription-PCR experiments led to the identification of three sesquiterpene synthases. By combination of in vitro and in vivo characterization of sesquiterpene synthase gene products in Escherichia coli and Saccharomyces cerevisiae, respectively, two enzymes were identified as germacrene A synthases, the key enzymes of sesquiterpene lactone biosynthesis. Due to the very low in vitro activity, the third enzyme was expressed in vivo in yeast as a thioredoxin-fusion protein for functional characterization. In in vivo assays, it was identified as a multiproduct enzyme with the volatile sesquiterpene hydrocarbon δ-cadinene as one of the two main products with α-muuorlene, β-caryophyllene, α-humulene and α-copaene as minor products. The second main compound remained unidentified. For expression studies, glandular trichomes from the anther appendages of sunflower florets were isolated in particular developmental stages from the pre- to the post-secretory phase. All three sesquiterpene synthases were solely upregulated during the biosynthetically active stages of the trichomes. Expression in different aerial plant parts coincided with occurrence and maturity of trichomes. Young roots with root hairs showed expression of the sesquiterpene synthase genes as well. Conclusion This study functionally identified sesquiterpene synthase genes predominantly expressed in sunflower trichomes. Evidence for the transcriptional regulation of sesquiterpene synthase genes in trichome cells suggest a potential use for these specialized cells for the identification of further genes involved in the biosynthesis, transport, and regulation of sesquiterpene lactones.

Sesquiterpene lactones (STLs) are a sub-class of sesquiterpenoids and are typical secondary compounds of the Asteraceae plant family [17,18]. Because of their diverse cytotoxic properties, these compounds are generally separated from the cellular metabolism and stored in specialized cells and compartments like laticifers or glandular trichomes [19,20]. STLs show anti-microbial activities and, due to their bitter taste, serve as anti-feedants [21][22][23]. From sunflower, Helianthus annuus, several STLs with germacranolide skeleton have been identified from capitate glandular trichomes, which are found on various aerial plant parts, particularly on leaves and anthers of the disk florets [24,25].
In the last several years, the potent bioactivities of STLs, such as anti-malarial activity of artemisinin [26], drew significant interests in the biochemistry of STLs. The first committed step in biosynthesis of all cyclic sesquiterpenes and STL is the cyclization of FDP by sesquiterpene synthases in the cytosol [24,25,27,28]. Germacrene A synthases, catalyzing the first step of STL biosynthesis [29], have been identified from different Asteraceae species in the young leaves of Ixeris dentata [30], etiolated chicory heads [31], or whole lettuce seedlings [32]. From a glandular trichome cDNA library of Artemisia annua, a germacrene A synthase has been identified which is the first and so far only sesquiterpene synthase which was isolated directly from secretory glandular cells [33].
On the leaves of Helianthus annuus, two different types of glandular trichomes are observed: uniserial glandular trichomes producing sesquiterpenes with bisabolene skeleton [34] and capitate glandular trichomes which secrete STL of the germacranolide type [35] and 5-deoxy-flavons [36] into an apical cuticular globe. Generally, trichomes on the leaves of Asteraceae plants develop in early leaf stages. This strongly complicates the isolation of these specialized chemical factories for identification of enzymes involved in sesquiterpene biosynthesis and does not allow a stage-specific analysis of gene expression and secretion process. Besides the sunflower leaves, the same glandular trichomes are found at the anther tips of disk florets [37]. Trichome development in these flower parts proceeds simultaneously with the floret and pollen growth, thus allowing an exact visual assessment of the trichome developmental stages [38]. It was shown by microscopic observations and HPLC analysis that secretion of STL takes place during the expansion of the subcuticular globe. Using a micropreparation technique, sunflower capitate glandular trichomes from anthers could be isolated in specific developmental stages ranging from the pre-to the post-secretory phase. The accessibility of differentially developed trichomes by means of this simple and effective micropreparation technique enabled us to use the sunflower inflorescence as a promising model system to study sesquiterpene lactone metabolism.
In this paper, we report the isolation of three sunflower sesquiterpene synthases and their functional characterization in vitro after expression in Escherichia coli and in vivo in Saccharomyces cerevisiae. Furthermore their expression patterns in different sunflower tissues and in different biosynthetic stages during trichome development is analyzed.

Identification of sunflower sesquiterpene synthases
Sunflower capitate glandular trichomes were isolated in the biosynthetically active stage from anther appendages as previously described [36]. Degenerated primers for the conserved sequences of sesquiterpene synthases [43] were used in reverse transcription (RT)-PCR to retrieve partial sesquiterpene synthase sequences. The deduced amino acid sequences of the two PCR fragments displayed high sequence similarity to other sesquiterpene synthase genes in the public database. The 3'-and 5'-rapid amplification of cDNA ends (RACE) led to the identification of two distinct full-length cDNAs.  [43][44][45].
For further systematic classification of the identified sesquiterpene synthases, the genomic DNA (gDNA) sequences were obtained. PCR amplification of gDNA with specific primers for HaTPS2 resulted in a single amplicon. The complete nucleotide sequence showed a length of 2,791 bp [GenBank: EU443250]. In contrast to HaTPS2, PCR amplification with primers for HaTPS1 showed two gDNA fragments which differed in length. Sequencing of both products resulted in the identification of a third sesquiterpene synthase that showed 95% deduced amino acid identity to the HaTPS1. Thus, the ORF of the HaTPS1 and its close homolog was designated as HaTPS1a and HaTPS1b [GenBank: EU327785], respectively. The gDNA sequences for HaTPS1a/b consisted of highly similar exon structures but differed considerably in their intron length and sequence in particular for the intron 2 and 4 ( Figure 1). The gDNA for HaTPS1a showed a length of 2,826 bp [GenBank: EU439590] while the gDNA for HaTPS1b contained 3,312 bp [GenBank: EU443249]. All three sunflower sesquiterpene synthase genes displayed an assembly of 7 exons and 6 introns, typical for class III terpene synthases [46]. They belong to the TPSa-gene family [45], a group of genes mainly consisting of angiosperm sesquiterpene synthases [47]. The ORF size in HaTPS1b was identical to HaTPS1a (559 amino acids) with 95% sequence identity. The HaTPS1a/b shared 63% amino acid identity with HaTPS2 and shared 94% similar-ity to germacrene A synthases from Asteraceae like Lactuca sativa (LsLTC1 and LsLTC2; [32]) or Cichorium intybus (CiGASlo [31]). Comparison of the deduced amino acid sequence of HaTPS2 with entries in public database showed 62% similarity to Asteraceae germacrene A synthases from lettuce or chicory but showed only about 40% identity to δ-cadinene synthases from Gossypium arboretum and G. hirsutum [48][49][50]. The amino acid sequences of all three sesquiterpene synthases contained the common sequence motifs for the sesquiterpene synthase family [45], such as the highly conserved DDxxD and RxR motifs, responsible for the divalent metal ion-substrate binding ( Figure 2).

Functional characterization of sunflower sesquiterpene synthases HaTPS2 (HaCS)
For functional characterization, all three synthase genes were heterologously expressed in E. coli as thioredoxinfusion proteins. The HaTPS2 fusion protein was purified through Ni-NTA affinity chromatography ( Figure 3a). The gas chromatograph mass spectroscopy (GC-MS) analysis of the in vitro enzyme assay products with substrate FDP showed the presence of the parental mass of sesquiterpene (m/z 204) but with a very low product yield (Figure 3b) which prevented the identification of conversion products. To solve this problem, HaTPS2 was expressed heterologously in vivo in S. cerevisiae using high-copy plasmid pESC-Leu2d in the EPY300 strain which was previously engineered to synthesize copious amount of FDP from simple carbon source [51,52]. The transgenes were Display of the exon-intron structure of HaTPS1a (HaGAS1), HaTPS1b (HaGAS2) and HaTPS2 (HaCS) genes  Alignment of the deduced amino acid sequences of sesquiterpene synthase genes Figure 2 Alignment of the deduced amino acid sequences of sesquiterpene synthase genes. HaTPS1a (HaGAS1), HaTPS1b (HaGAS2), and HaTPS2 (HaCS) indicate sesquiterpene synthase genes isolated from sunflower in this study. LsLTC2, CiGAS, and TEAS are germacrene A synthase from Lactuca sativa, germacrene A synthase from Cichorium intybus, and 5-epi-aristolochene synthase from Nicotiana tabacum, respectively. Black: identical amino acids in all sequences; dark grey and light grey: identical amino acids in 5 or 4 enzymes, respectively.

DDxxD RxR
induced by the addition of galactose to the medium, and non-volatile dodecane was overlaid on the culture to trap volatile sesquiterpene products. The GC-MS analysis of recombinant enzyme product showed a much higher product yield compared to the in vitro assay (Figure 3), but the fragmentation pattern of the obtained products still did not allow unambiguous product identification. In this analysis, significant accumulation of farnesol was detected in the negative control ( Figure 4, peak I) and in the products obtained by expression of HaTPS2 in EPY300, indicating an incomplete conversion of FDP to sesquiterpenes. To improve the activity of the enzyme, HaTPS2 was expressed as a thioredoxin-fusion protein (thioredoxin-HaTPS2, Figure 4) in EPY300, similar to the expression in E. coli. This modification resulted in a notable increase of synthesized products without altering the overall product profile. Farnesol was completely absent in this in vivo assay, indicating a complete conversion of FDP to sesquiterpenes probably due to the significantly enhanced HaTPS2 activity. Products of HaTPS2 were identified by comparison with the reference spectra in the NIST02 library and also with the authentic standards.
One of the two main products was identified as δcadinene with α-muurolene, β-caryophyllene, α-humulene and α-copaene as minor products. This result characterized HaTPS2 as an Asteraceae sesquiterpene synthase involved in the biosynthesis of mainly δ-cadinene and other minor sesquiterpenes (Figure 4), and hence it was named as HaCS (Helianthus annuus Cadinene Synthase). The second main product remained unidentified, but its fragmentation pattern (Figure 4, peak 7) showed high similarities to that of an authentic γ-cadinene standard, but differed slightly in the retention time indices (RIs). The RI of authentic γ-cadinene was 1519, whereas the RI of peak 7 was 1521. Spiking the yeast terpene products  Peak numbers in each panel correspond to numbers above peaks in the chromatogram. Mass spectra of identified products are shown in the top of each panel with a mass spectrum of an authentic standard below. One of the two main products was identified as δ-cadinene; the fragmentation pattern of peak 7 showed high similarities to γ-cadinene but differed slightly in the retention time. RI: retention indice. I: farnesol. with the standard showed a fused, broader peak than the original peak 7 or γ-cadinene standard, indicating that peak 7 is not γ-cadinene (data not shown). Comparison of this fragmentation pattern with that of δ-cadinene and γcadinene indicates that this so far unidentified compound is also a product with a cadinene skeleton, but its chemical identity remains unknown. The fragmentation patterns of peaks 4 and 5 were very similar to each other ( Figure 4) and both showed high similarities to the fragmentation pattern of γ-gurjunene from the NIST02 library.
Multiproduct terpene synthases are known from different plant families. For example, γ-humulene synthase and δselinene synthase from Abies grandies produce 52 and 34 products, respectively, from the FDP [53]. Within Asteraceae, only the epi-cedrol synthase from Artemisia annua [42] has been described to produce more than one product in significant amount. The only other two sesquiterpene synthases producing sesquiterpenes with the cadinene skeleton were found in Gossypium hirsutum and G. arboretum [48,49,54,55].
GC-MS analysis of pentane extracts from isolated glandular trichomes showed small amounts of sesquiterpenes. Peaks representing α-copaene, β-caryophyllene, δcadinene and the unidentified compounds 7 and 9 (Figure 4) were detected. SPME headspace-analysis of sunflower volatiles showed a whole bouquet of different mono-and sesquiterpenes. Again sesquiterpenes produced by HaCS were detected, this time in larger amounts (data not shown). These results are in accordance with the report from Schuh et al. [56] where β-caryophyllene and α-humulene were detected as volatile emissions from H.
annuus. Besides the compounds produced from HaCS, several other sesquiterpenes were detected in the headspace experiments, indicating the activity of yet unidentified terpene synthases in sunflower. Generally, terpene synthases form a large gene family [47,57], and hence it is evident that more terpene synthases are expressed in different sunflower tissues or still remained unidentified in glandular trichomes.

Functional identification of HaTPS1a and HaTPS1b as germacrene A synthases (HaGAS)
HaTPS1a and HaTPS1b were expressed as thioredoxin fusion proteins in E. coli to produce soluble protein.
In vitro enzyme assays with purified enzyme showed a single product with the expected mass of m/z 204 in the GC-MS measurements ( Figure 5a). A peak with the same retention time and fragmentation pattern was observed by the analysis of a germacrene A standard, produced by the expression of the previously characterized germacrene A synthase LsLTC2 from Lactuca sativa [32]. The identity of the peak from the in vitro assays was determined as β-elemene by an authentic standard. β-elemene is the known cope-rearrangement product of germacrene A due to the hot injection port temperature for GC measurements [29,32]. Thus, the HaTPS1a and HaTPS1b were named as HaGAS1 and HaGAS2, respectively.
The performance of both sunflower germacrene A synthases was tested in vivo by expression in yeast and for comparison of the product spectra between in vitro and in vivo expression. HaGAS1 and HaGAS2 were cloned into the pESC-Leu2d plasmid and used to transform S. cerevisiae EPY300. To produce the germacrene A reference standard, a previously characterized germacrene A synthase from Lactuca sativa (LsLTC2 [32]) was also cloned and expressed under the same conditions. GC-MS analyses of these products showed two identical peaks for all three germacrene A synthases ( Figure 5b). The earlier eluting peak was identified as β-elemene, whereas the later peak represented germacrene A, as it showed identical fragmentation pattern to the literature data and terpene products from reference gene (i.e. LsLTC2) [29,31,32]. In contrast to the GC-MS measurements of the in vitro assays, germacrene A produced from in vivo assays was not completely converted to β-elemene. The complete rearrangement of germacrene A to β-elemene depicted in Figure 5a is likely due to the high injection port temperature used (280°C for Figure 5a versus 250°C for Figure 5b). These results showed that HaGAS1 and HaGAS2 are germacrene A synthases, which catalyze the first committed reaction for sesquiterpene lactone biosynthesis in sunflower. In contrast to HaCS, both germacrene A synthases are single product enzymes.

Biochemical characterization of sunflower germacrene A synthases
To obtain native HaGAS1 and HaGAS2 enzymes, the E. coli-expressed fusion proteins were digested with enterokinase and further purified to remove the thioredoxin and the enterokinase before biochemical characterization. SDS-PAGE showed nearly homogeneous proteins. For determination of the influence of the thioredoxin fusion part on the biochemical properties of HaGAS1, thioredoxin-HaGAS1 fusion protein was also characterized. HaCS did not show reproducible results in vitro and its biochemical properties were not determined.  [58,59]. These results indicate that the fused thioredoxin protein part has only minor effects on the properties and catalytic activity of HaGAS1. In contrast to thioredoxin-HaGAS1 and native HaGAS1, HaGAS2 showed substrate inhibition characteristics; because the activity decreased with substrate concentrations above 2.5 μM. Substrate inhibition has also been reported for monoterpene synthases from Citrus limon [60].

Tissue-specific expression of sunflower sesquiterpene synthases
In order to assess the relative transcript level of sesquiterpene synthase, semi-quantitative RT-PCR experiments were carried out using cDNA templates from roots, stem, cotyledons, young and old leaves, ray flowers, and trichomes ( Figure 6a). The transcript level for farnesyl diphosphate synthase (FDPS) was also analyzed as this enzyme provides the substrate farnesyl diphosphate (FDP) for the sesquiterpene synthases. It could be shown that FDPS was expressed in all tissues observed, but was upregulated in those tissues where expression of sesquit-erpene synthase genes was detected at highest level (Figures 6,7,8). Due to very high sequence similarity, the transcripts for HaGAS1 and HaGAS2 could not be distinguished in the RT-PCR analyses. Therefore, PCR products for HaGAS represent the transcripts from both germacrene A synthase genes. The FDPS was detectable with different expression intensity in all organs except for cotyledons. HaGAS and the HaCS showed a much more differentiated expression. Transcripts of all three genes were traceable in roots and young leaves, but in contrast to HaGAS, HaCS was not expressed in old leaves. The strongest expression of all three genes was detected in capitate glandular trichomes. These results showed the predominant expression of sunflower sesquiterpene synthase genes in the highly specialized trichome stalk cells. As the transcript for FDPS was also detectable in RNA samples from these secretory cells, it is predicted that the whole biosynthetic pathway from the precursor molecules to the secreted STL GC-MS analysis of sesquiterpene products of sunflower germacrene A synthases HaGAS1 and HaGAS2 compared to germa-crene A standard produced by LsLTC2 from lettuce is located in the sunflower trichomes. Transcripts detected in the leaf samples are likely to show the expression of the sesquiterpene synthase genes in trichomes located on the leaf epidermis. The expression of the sesquiterpene synthase genes in young roots, even though low in comparison to the expression in trichomes, showed that both HaGAS1/2 and HaCS were not exclusively expressed in the aerial parts of the sunflower. These results were confirmed by quantitative real-time RT-PCR experiments (Figure 8). Expression of terpene synthases in roots has been described for many plant species and seems to be a common biosynthetic process in plants [57,58,[61][62][63].
As the HaGAS1 and HaGAS2 were not distinguishable by PCR, a restriction digestion was performed to confirm the expression of both genes. The full-length cDNA of both synthases was amplified from root and trichome cDNA with the same primer pair, and their amplicons were subjected to the differential restriction enzyme digestion. The restriction enzyme DraI was identified to cut only HaGAS1 but not HaGAS2, PauI cut HaGAS2 but not HaGAS. Gel separation of the digested products clearly demonstrated the expression of both germacrene A synthases in roots and trichomes (Figure 6b).

Two lettuce germacrene A synthases were induced in cotyledons by infection with the downy mildew pathogen
Bremia lactucae [32]. In contrast, inoculation of sunflower seedlings with Plasmopara halstedii [64], the sunflower downy mildew pathogen, did not induce the expression of sunflower sesquiterpene synthases in cotyledons or stem and did not have influence on the expression intensity in roots (data not shown). This indicates that expression of these genes is tightly regulated by the developmental program of the trichome secretory cells and is not induced by the infection with these pathogens.

Developmentally regulated expression of sesquiterpene synthase genes in sunflower capitate glandular trichomes
The low density of glandular trichomes and the early termination of the secretory active phase in young leaf stages complicate the isolation of biosynthetically active trichomes from sunflower leaves. However, trichomes from the anther appendages provided an excellent alternative to study developmental gene expression along the trichome development [38]. Using glands from disk florets of different ages, trichome RNA could be isolated from the pre-secretory to post-secretory stage as previously described [36]. In order to assess transcriptionally active stages of sunflower trichome cells, semi-quantitative RT-PCR experiments were performed to amplify HaGAS, HaCS, and FDPS (Figure 7). While FDPS was expressed with the same level in all but the last trichome stage, HaGAS and HaCS were highly regulated and expressed only during the active secretory phase of the capitate glan-Expression of sesquiterpene synthase genes in different sun-flower tissues dular trichomes. Real-Time RT-PCR data indicated that the combined expression level of HaGAS1 and HaGAS2 seems to be significant higher than that of HaCS ( Figure  8). A stage-specific expression of terpene synthase genes during trichome development has not been reported before. These semi-quantitative RT-PCR results are in line with the biosynthetically active phases of sunflower trichomes determined by microscopic observations of fluorescent flavones in trichomes and HPLC-measurements of STL content [36,38].

Conclusion
Three sesquiterpene synthase genes isolated from pure trichomes were functionally expressed in E. coli and S. cerevisiae. The terpene product analysis by GC-MS showed that they encode two distinct types of sesquiterpene synthases -germacrene A synthase (HaGAS1/2) and δcadinene synthase (HaCS). The expression of HaGAS1/2 and HaCS in engineered yeast significantly increased the yield of terpene production and thus benefited the identification of terpenes without the need to purify enzymes and to use expensive substrate. All three sesquiterpene synthases were predominantly expressed in the stalk cells of capitate glandular trichomes at active secretory stages. The use of trichomes from anther appendages provides an excellent model system for the analysis of differentially expressed genes during STL biosynthesis. Since the trichomes can be isolated at specific biosynthetic stages, the isolated trichomes can be used to generate expressedsequence tags or microarray probes, hence providing valuable experimental tools to study STL biochemistry in Asteraceae.

Plant materials, RNA isolation, and cDNA synthesis
Helianthus annuus L. cv. HA300 and Lactuca sativa var. capitata plants were grown under greenhouse conditions with an additional 16 h illumination (330 μmol s -1 m -2 ) and a night length of 8 hours. H. annuus capitate glandular trichomes were mechanically isolated from anther appendages as previously described [38]. Studies on the organ development revealed that the formation of trichomes on anthers starts early and parallels the consecutive centripetal maturation of florets within the capitulum. Different trichome stages were determined microscopically by direct analysis of trichomes and pollen development; and light microscopic images were taken as previously described [38].
For total RNA extraction, trichomes were isolates from fresh plant material and immediately transferred to 200 μl ice-chilled RNA extraction buffer (Aurum total RNA isolation Kit, Biorad, Munich, Germany) in 2 ml vials. A mixer mill (MM20, Retsch, Haan, Germany) was used for cell disruption (16 Hz, 1 min) using 2 ceramic beads (2.8 mm diameter, Precellys, Peqlab, Erlangen, Germany). After cell disruption, an additional 500 μl lysis buffer was added. All subsequent steps were carried out as described in the manual. For total RNA isolation from pure glands, glandular trichomes from 200 florets (approx. 30,000 to 40,000 trichomes) were used. For the purification of RNA from different trichome stages, the glands from anther appendages of 50 florets were isolated. RNA quantity and integrity was verified by the Bioanalyzer 2100 using a RNA 6000 Pico Chip (Agilent, Böblingen, Germany). For routine PCR, cDNA was synthesised from total RNA using the RevertAid First Strand cDNA Synthesis Kits (Fermentas, St. Leon-Rot, Germany) with VNdT 18 primer.

Identification of HaGAS1 and HaCS
For identification of sesquiterpene synthase genes by PCR, degenerate primers were used to obtain fragments of sunflower sesquiterpene synthases (forward primer, 5'-GAY GAR AAY GGI AAR TTY AAR GA-3', and reverse primer, 5'-CCR TAI GCR TCR AAI GTR TCR TC-3' [43]). PCR was performed in a total volume of 25 μl containing 1 μmol of the two primers, 0.25 μmol dNTPs, 1 unit of Taq DNA Polymerase (Fermentas, St. Leon-Roth, Germany) and 2 μl of cDNA. The PCR reaction was performed on a Mastercycler Gradient (Eppendorf, Hamburg, Germany) with 3 min of initial denaturation at 94°C, followed by 35 cycles of 1 min denaturation, 1 min annealing at 42°C, and 2 min of elongation at 72°C. Agarose gel electrophoresis showed a single band with a length of approximately 500 bp. Separation of the same PCR-reaction on 10% polyacrylamide gel revealed two bands with approximately 560 and 600 bp in length. Both bands were excised and transferred to 2 ml reaction tubes. After the addition of 150 μl ddH 2 O, the gel fragments were disrupted in a mixer mill for 1 min at 10 Hz using 2 ceramic beads. After centrifugation (5 min, 10,000 g), the supernatant was removed and used directly for reamplification of the PCR fragments with the same primer pair as before. The PCR products were gel-purified (Qiagen Gel Extraction Kit, Hilden, Germany) and used for direct sequencing using the same primers.
To obtain full length sequences, 3'-RACE was performed according to the protocol of Sambrook & Russell [65]. The reaction was carried out in a thermocycler with 5 min initial denaturation (94°C), 5 min of annealing at 49°C and a first elongation for 40 s at 72°C, followed by 30 cycles with 40 s denaturation (94°C), 1 min annealing (49°C), and 3 min elongation at 72°C. The PCR amplifications and subsequent direct sequencing of the resulting fragments were carried out using the RACE-adapter primer While amplification of HaCS gDNA revealed a single band, the PCR amplification of HaGAS1 resulted in two products with different length. The PCR products were gel-purified using the illustra GFX PCR Gel Band Purification Kit (GE Healthcare GmbH, Munich, Germany) and cloned into the pSC-A plasmid by UA-cloning following the instructions of the StrataClone PCR Cloning Kit (Strat-agene Inc., La Jolla, USA). The fragments were sequenced. Sequences were aligned using the Seqman module of the DNASTAR software package (Lasergene, Madison, WI, USA). Introns and exons were identified by comparison of the gDNA sequences with the previously established mRNA sequences for HaGAS1 and HaCS. Both PCR products for HaGAS1 with the same primer pairs showed highly similar sequences in the exon parts but differed within the intron sequences in length and nucleotide composition. This resulted in the identification of a second germacrene A synthase gene (HaGAS2). For all sequencing work the sunflower cultivar HA300 was used, but the presence of all three sesquiterpene synthase genes was verified in wild type H. annuus (data not shown).

Detection of the HaGAS1 and HaGAS2 transcripts in trichomes and roots
The full length coding sequence for HaGAS1 and HaGAS2 was amplified from trichome and root cDNA using the forward primer 5'-ATG GCA GCA AGT TGG AGC CAG-3' and the reverse primer 5'-TTA CAT GGG TGA AGA ACC AAC AAA C-3'. The PCR conditions were 2 min initial denaturation followed by 30 cycles of 40 s denaturation (94°C), 40 s annealing (60°C), 2 min 20 s elongation (72°C). The PCR amplicons were gel-purified. To distinguish between HaGAS1 and HaGAS2 amplicons, a restriction digestion was performed using PauI and DraI (Fermentas GmbH). The HaGAS1 contains a PauI but no DraI recognition site while HaGAS2 contains a DraI site but no PauI recognition site.

Semi-quantitative RT-PCR and real-time quantitative RT-PCR
The cDNAs for RT-PCR were generated as described above.
The constitutively expressed ubiquitin mRNA [68] served as the reference transcript. After reverse transcription, each cDNA sample was diluted several fold and used for PCR with the forward primer 5'-CAA AAC CCT AAC CGG AAA GA-3' and the reverse primer 5'-ACG AAG ACG GAG GAC GAG-3' to amplify ubiquitin cDNA. Equal initial cDNA concentrations within different samples were defined by equal amplification intensity for the ubiquitin transcript. PCR-Cycle number for PCR reactions was chosen to be in the linear range. Transcipts for HaGAS1 and HaGAS2 were traced by amplification with the forward primer 5'-TTG AGA TTG AAA GGG GAA AAC-3' and the reverse primer PCR-experiments PCR products were separated on 1% agarose gels, stained for 30 min in a water bath containing 1.5 μg ml -1 ethidium bromide and exposed to UV light (312 nm) for documentation.
For quantitative real-time PCR 5× master mixes (LightCycler FastStart DNA Master PLUS SYBR Green I, Roche Diagnostics GmbH, Mannheim, Germany) were used according to manufacturer's recommendations on a Light-Cylcer 1.5 instrument (Roche Diagnostics GmbH). Same primer combinations as for semi-quantitative RT-PCR were used. Initial denaturation time for all samples was 10 min (95°C), followed by 50 cycles with 10s annealing (57°C for FPPS and HaCS, 59°C for HaGAS, 60°C for Ubiquitin), 20s elongation (72°C). Ramp temperature was set to 20°C/s. The melting curve of all samples was analyzed. Additionally, all reactions were loaded on 1% agarose gels to ensure amplification of products with the expected length. Relative gene expression was calculated using the Pfaffl method [69] with Ubiquitin as the reference transcript and compared to the expression levels of the specific transcripts of cDNA obtained from RNA isolations of glandular trichomes in presecretory stage. Rosetta-gami B LB-plates kanamycin (15 μg/ml) and tetracycline (12.5 μg/ml) was also added.

Heterologous expression of sesquiterpene synthase genes in E. coli and recombinant protein purification
LB-medium supplemented with carbenicillin (100 μg/ml) and chloramphenicol (34 μg/ml) was inoculated with 250 μl of an overnight culture of E. coli containing either pET32::HaGAS1, pET32::HaGAS2, or pET32::HaCS and grown at 37°C to an OD of 0.5 -0.7 (600 nm). Cultures expressing HaGAS1 or HaGAS2 were shifted to 30°C over 30 min, induced with 0.5 mM isopropyl-β-d-thiogalactopyranoside (IPTG) and incubated for 4 h at 30°C (220 rpm). Cultures expressing HaCS were treated in the same way, but were shifted to 10°C over 45 min, induced with 0.1 mM IPTG and cultivated for a further 24 h at 10°C (180 rpm).
E. coli cells were concentrated by centrifugation (9,000 g, 5 min, 4°C) and proteins were extracted using 5 ml Bug-Buster Protein Extraction Reagent (Novagen) per g bacteria cells. 1 μl Benzoase (Novagen) per ml was added and the assay was incubated at room temperature (RT) for 20 min in a shaker (100 rpm). Insoluble proteins and cell fragments were removed by centrifugation (9,000 g, 25 min, 4°C) and the supernatant was cleared using a 0.45 μm filter. To every 5.0 ml protein extract 1.0 ml pre-equilibrated Ni-NTA agarose (Novagen) was added and incubated at 4°C for 60 min in a shaker. The complete slurry was transferred to chromatography column and the supernatant with the unbound proteins was removed. The agarose was washed twice with ice-cold wash buffer (50 mM NaH 2 PO 4 , 300 mM NaCl, 20 mM imidazol, pH 8.0), followed by a single washing step with 100 mM imidazol (same buffer as before). For elution of the 6x-His Tag proteins ice-cold 250 mM imidazol was used (same buffer as before). Soluble proteins were analyzed by SDS-PAGE.
Proteins were concentrated by ultracentrifugation (

Protein expression in vivo in S. cerevisiae
For in vivo expression of the sesquiterpene synthases, the genes were cloned into the pESC-Leu2d plasmid [52]. As no germacrene A standard was available, the previously characterised germacrene A synthase (LsLTC2) from Lactuca sativa [32] was amplified from lettuce cDNA using the primer pair: forward primer 5'-ACG TGG ATC CAA CAT GGC AGC AGT TGA CAC TAA TG-3' and reverse primer 5'-ACG TGC TAG CTT ACA TGG ATA CAG AAC CAA C-3'. PCR reactions were performed using Phusion DNA Polymerase (New England Biolabs). HaGAS2, HaCS, and LsLTC2 were cloned into MCS2 of the pESC-Leu2d plasmid, HaGAS1 was cloned into MCS1 of the pESC-Leu2d plasmid. All amplicons were digested with the appropriate restriction enzymes overnight at 37°C, gel purified, and ligated into the pESC-Leu2d plasmid.
For all in vivo expression experiments, EPY300 S. cerevisiae cells were used. These cells were engineered for a high level of FDP production [51,52]. EPY300 were transformed with purified plasmids following the protocol from Gietz & Woods [71]. For protein expression, 5 ml SC medium (without Met, His, Leu and with 2% glucose supplement) were inoculated with single colony and grown overnight at 30°C (200 rpm). 250 ml culture flasks containing 50 ml YPAD medium (0.2% glucose, 1.8% galactose) supplemented with 1 mM methionine were inoculated with 1 ml overnight culture and overlaid with 5 ml of dodecane (Sigma-Aldrich GmbH, München, Germany). After 3-4 days incubation at 30°C (200 rpm), the cultures were transferred to 50 ml falcon tubes and centrifuged at 10,000 g (5 min). The dodecane overlay was carefully removed, diluted 1:100 with ethyl acetate and used directly for GC-FID and GC-MS analyses.

Identification of products of enzyme expression in vivo in
S. cerevisiae and of in vitro assays GC-MS analyses of terpenes produced by recombinant enzyme in S. cerevisiae were performed on an Agilent 6890N gas chromatography system coupled to an Agilent 5975B mass spectrometer. In vitro assays with recombinant HaGAS1 and HaGAS2 protein, produced by heterologous expression in E. coli, were analyzed on an Agilent 6890N gas chromatograph coupled to an MS5973 mass spectrometer (Agilent). 1 μl samples were injected at 250°C and analysed on a HP-5MS column (30 m × 250 μm i.d. × 0.25 μm film thickness, Agilent). Helium (constant flow rate of 1 ml min -1 ) was used as carrier gas. The temperature program was 40°C for 1 min followed by a linear gradient of 10°C min -1 to 250°C. Terpenes produced by in vitro assays with heterologously expressed HaCS were analyzed on a GC3400 gas chromatograph (Varian GmbH, Darmstadt, Germany) coupled to a Saturn 4D ion-trap mass spectrometer (Varian). 1 μl injection at a temperature of 250°C, He as carrier gas with 1.0 ml min -1 . Temperature program: 50°C for 2 min, then 10°C min -1 to 300°C, which was held for 3 min. Spectra were interpreted with NIST02 Mass Spectra Library (Wiley & Sons, Mississauga, Canada). Reference compounds were obtained from Sigma-Aldrich (δ-cadinene) or Fluka (β-Caryophyllene, Buchs, Swiss). α-copaene, α-humulene and α-muurolene were identified by comparison with characterized compounds of Aloysia sellowii oil (generous gift from J. Degenhardt, Jena). Alkane standard (C8-C20, Fluka) was used to determine retention indices.
For analysis of sesquiterpenes present in glandular trichomes, approximately 10,000 glandular trichomes were extracted with pentane (1 ml) for 10 min. The pentane extract was carefully concentrated using nitrogen gas and analysed by GC-MS. Headspace trap experiments for detection of emitted terpenes were done using solid phase micro extraction (SPME). Two young flower heads were placed in an Erlenmeyer flask covered with aluminium foil. After one hour the SPME fibre was placed in the flask together with the flower heads for 60 minutes and trapped volatiles were analysed by GC-MS (data not shown).
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