Accumulation of phenanthrene by roots of intact wheat (Triticum acstivnm L.) seedlings: passive or active uptake?

Background Polycyclic aromatic hydrocarbons (PAHs) are of particular concern due to their hydrophobic, recalcitrant, persistent, potentially carcinogenic, mutagenic and toxic properties, and their ubiquitous occurrence in the environment. Most of the PAHs in the environment are present in surface soil. Plants grown in PAH-contaminated soils or water can become contaminated with PAHs because of their uptake. Therefore, they may threaten human and animal health. However, the mechanism for PAHs uptake by crop roots is little understood. It is important to understand exactly how PAHs are transported into the plant root system and into the human food chain, since it is beneficial in governing crop contamination by PAHs, remedying soils or waters polluted by PAHs with plants, and modeling potential uptake for risk assessment. Results The possibility that plant roots may take up phenanthrene (PHE), a representative of PAHs, via active process was investigated using intact wheat (Triticum acstivnm L.) seedlings in a series of hydroponic experiments. The time course for PHE uptake into wheat roots grown in Hoagland solution containing 5.62 μM PHE for 36 h could be separated into two periods: a fast uptake process during the initial 2 h and a slow uptake component thereafter. Concentration-dependent PHE uptake was characterized by a smooth, saturable curve with an apparent Km of 23.7 μM and a Vmax of 208 nmol g-1 fresh weight h-1, suggesting a carrier-mediated uptake system. Competition between PHE and naphthalene for their uptake by the roots further supported the carrier-mediated uptake system. Low temperature and 2,4-dinitrophenol (DNP) could inhibit PHE uptake equally, indicating that metabolism plays a role in PHE uptake. The inhibitions by low temperature and DNP were strengthened with increasing concentration of PHE in external solution within PHE water solubility (7.3 μM). The contribution of active uptake to total absorption was almost 40% within PHE water solubility. PHE uptake by wheat roots caused an increase in external solution pH, implying that wheat roots take up PHE via a PHE/nH+ symport system. Conclusion It is concluded that an active, carrier-mediated and energy-consuming influx process is involved in the uptake of PHE by plant roots.


Background
Polycyclic aromatic hydrocarbons (PAHs) are a group of organic compounds composed of two or more fused aromatic rings in linear, angular, or cluster arrangements [1]. PAHs are of particular concern because of their hydrophobic, recalcitrant, persistent, potentially carcinogenic, mutagenic and toxic properties, and their ubiquitous occurrence in the environment.
Over 90% of PAHs in the environment reside in surface soil [2]. Furthermore, plants grown in PAH-contaminated soils can become contaminated with PAHs due to their absorption [3][4][5][6]. Therefore, they may pose human and animal health hazards. Previous studies have shown that dietary intake of PAHs can be a significant route of exposure to the general population where vegetables and grains are a major source of dietary PAHs [7,8]. It is thus important to understand exactly how PAHs are transported into the plant root system and into the human food chain, since it is helpful to produce PAH-free crop products by means of genetic engineering, to remove PAHs from PAH-polluted soils or water through phytoremediation, and to model potential uptake for risk assessment.
Over the last three decades, organic compounds uptake by roots, particularly pesticides/herbicides, has been widely studied [9][10][11][12][13][14]. Plant roots can take up organic contaminants via passive diffusive partitioning (i.e. apoplastic) and/or active (i.e. symplastic) process, depending on the properties of the organic contaminant and the plant species. Passive transport proceeds in the direction of decreasing chemical potential; nevertheless, active transport is against the chemical potential gradient, requiring expenditure of energy [15]. The absorption of non-ionized organic compounds by roots of higher plants is generally thought to be a passive, diffusive, partitioning and nonmetabolic process [16]. Uptake of non-ionized organic compounds is influenced by the properties of the contaminant [17,18]. Briggs et al. [9] established a linear relationship between the octanol/water partition coefficient (K OW ) of non-ionized chemicals and the observed root concentration factor (RCF, i.e., chemical concentration in the root/concentration in external solution) from their experiments involving in the uptake of Omethylcarbamoyloximes and substituted phenylureas into barley plants. With consideration of the passive transport of non-ionic organic pollutants into plants (including crops), a partition-limited model has been proposed to estimate the concentration of a contaminant in plants [19]. However, during further test of the partition-limited model through uptake of PAHs by plant roots, some researchers observed that predicted and measured values of PAH content in plant roots fitted well at low PAH concentrations in soils or hydroponic solution, whereas the prediction error was considerably large, with a maximum of more than 81%, at high PAH concentration in soils or hydroponic solution [20,21]. Collins et al. [22] pointed out that looking at root uptake of non-ionic organic chemicals purely as a partitioning process may be incorrect as this assumes it is independent of concentration, which it is not when the roots become saturated. Wild et al. [23] visualized the occurrence of anthracene and phenanthrene within the root cell vacuole using two-photon excitation microscopy. All of the above-mentioned seem to indicate that the passive uptake cannot satisfactorily account for the transport process of PAHs into plant roots.
Despite much work regarding uptake and accumulation of non-ionized organic compounds like PAHs in plants conducted previously, mechanisms for PAHs uptake by plant roots and translocation in plants still remain unclear, in particular, whether active transport is involved in root uptake of PAHs and what the proportion of active transport to the total uptake of PAHs by roots is.
In this paper, it is hypothesized that there are two general mechanisms for PAHs uptake and transport, i.e. a passive and an active component coexisting in higher plants with their relative contribution being dependent much upon the plant species and PAH levels. The objectives of this study are i) to confirm whether active transport is involved in the uptake of PAHs by plant roots and ii) to evaluate the relative contribution of active and passive components with respect to PAHs uptake and transport processes in higher plants. To our knowledge, this is the first report to demonstrate that an active component is involved in PAHs uptake.

Absorption of PAHs as a function of time
To investigate uptake of PHE, wheat roots were exposed to 5.62 μM PHE for differing lengths of time and the amount of PHE that passed into the root was determined. Figure 1 shows the time-dependent PHE accumulation in roots of intact wheat seedlings. PHE uptake by wheat roots was nonlinear over 36 h. Wheat roots continued to accumulate PHE through 36 h. A characteristic immediate, rapid rate of uptake was followed by continued uptake but at a decreasing rate. PHE uptake rates between 0 (the initial) and 2 h, and 2 and 36 h were 16.0 ± 4.18 and 2.03 ± 0.06 nmol g -1 fr wt h -1 , respectively. PHE uptake rate in the first period (0 to 2 h) was almost 8 times higher than that in the period of 2 to 36 h.

Effect of temperature
Concentration-dependent uptake of PHE by wheat roots has features of saturating kinetics within the range of 7.3  Where V max (nmol g -1 fresh weight h -1 ) is the maximal transport rate when all available carrier sites are loaded, C (μM) is the external concentration of phenanthrene, K m (μM) is the Michaelis constant, equal to the substrate concentration giving half the maximal transport rate. Generally, the lower the K m value, the stronger the affinity between carrier and substrate carried [15]. The apparent K m value (derived from Lineweaver-Burk data transformations) for the saturable curve was 23.7 μM, significantly higher than the PHE solubility in water (7.3 μM, at 25°C). The V max value was 208 nmol g -1 fresh weight h -1 .
The uptake of PHE was significantly temperaturedependent ( Figure 3). Absorptions of PHE at 25°C were markedly higher than at 4°C (paired t-test, 95% confidence level), suggesting that low temperature (4°C) inhibited the uptake of PHE by wheat roots compared with PHE uptake at 25°C. The inhibition by low temperature became stronger with an increase in PHE concentration in hydroponic solution. The inhibition effect of the low temperature was slight for the treatment with PHE concentrations of 0-2.25 μM, whereas the inhibition effect was strengthened for the treatment with PHE concentrations of 2.25-6.74 μM. The inhibition rate, i.e., (uptake of PHE at 25°C -uptake of PHE at 4°C) × 100/uptake of PHE at 25°C, ranged from 13.70% to 36.87%.

Effect of 2,4-dinitrophenol
In general, dinitrophenol (DNP) is considered primarily as an uncoupler of oxidative (and, to a lesser extent, photosynthetic) phosphorylation, and of proton-coupled fluxes at the plasma membrane and endomembranes via the dissipation of transmembrane electrochemical gradients of protons. The effect of 2,4-DNP, a common metabolic inhibitor, upon the uptake of PHE was investigated in this study. Uptake of PHE was strongly depressed by 1 mM 2,4-DNP but was not completely inhibited ( Figure 4). The decreased PHE uptake induced by 2,4-DNP was statistically pronounced with paired t-test, at 95% confidence level. Similar to the inhibition effect of low temperature on uptake of PHE, the depression effect of 2,4-DNP also exhibited a dependence on PHE concentration. At PHE concentrations of 0-2.25 μM, the inhibition effect of 2,4-DNP was lighter. But the inhibition effect of 2,4-DNP was much stronger at PHE concentrations of 2.25-6.74 μM with rates between 19.47% and 35.60% (Figure 4).

Effect of naphthalene
Most of the studies regarding PAH uptake by plant roots have hitherto focused on an individual PAH [23][24][25]. However, PAH uptake by plant roots with the presence of two or more types of PAHs is less addressed. PHE uptakes by wheat roots with the absence and presence of naphthalene (NAP) are presented in Figure 5. Although the water solubility of NAP (247.3 μmol L -1 ) is much higher than that of PHE (7.3 μmol L -1 ) [26], wheat roots took up more PHE than NAP ( Figure 5) with PHE uptake 1.32 times more than that of NAP. The presence of NAP inhib-  ited PHE uptake, in turn, PHE also inhibited NAP uptake. Uptake of PHE decreased by as much as 70.1% with the presence of NAP, whereas NAP uptake decreased by 51.2% in the presence of PHE. The inhibition rate exhibited a similar trend. The inhibition rate of PHE uptake by NAP, i.e., (uptake of PHE by wheat roots treated with PHE -uptake of PHE by wheat roots treated with PHE and NAP) × 100/uptake of PHE by wheat roots treated with PHE, was 29.9%, and that of NAP uptake by PHE, i.e., (uptake of NAP by wheat roots treated with NAPuptake of NAP by wheat roots treated with PHE and NAP) × 100/uptake of NAP by wheat roots treated with NAP, was 48.8%. Both decrease in uptake and the inhibition rate revealed that the inhibition of NAP uptake by PHE was stronger than that of PHE uptake by NAP. Therefore, competitive inhibition occurs when 2 or more types of PAH are present in culture solution.

Effect of pH
The interaction between protons (H + ) and other cations or anions is of general importance for plant mineral nutrition. Thus, external solution pH has received much attention during absorption of compounds by plant roots [27,28]. In the present work, PHE uptake by wheat roots caused significant increase in external nutrient solution pH (Table 1). Moreover, ΔpH (i.e., nutrient solution pH after 4 h of PHE uptake minus the initial nutrient solution pH) increased with the increase of PHE uptake by wheat roots. For example, ΔpH is 0.21 in the control, about a 2fold increase in the treatment with 2.81 μM PHE, and an increase factor of 3 in the treatment with 5.62 μM PHE.
In the control, the increase in nutrient solution pH resulted from a NO 3 -/H + symport [29]. Obviously, nutrient solution pH increase in the other two treatments was caused by PHE uptake when compared with the control. The change in pH during uptake of phenanthrene by wheat roots in Millipore water further confirmed this phenomenon (Table 1).

Discussion
In contrast to uptake of ions by plant roots, mechanisms for uptake of non-ionic organic chemicals like PAHs still remain poorly understood. Chemicals may enter plant roots through passive and/or active process and this process is much dependent on the types of plant and chemical, and chemical level in solution. Passive uptake is a nonmetabolic, 'downhill' process, driven by diffusion or mass flow. However, active uptake is an 'uphill', energyconsuming process against the gradient of potential energy. It is generally accepted that the uptake of anthropogenic organic chemicals by plant roots is a passive, diffusive process [16,22,30]. Thus, the uptake of PAHs by plant roots is assumed to be a passive, partitioning process [31]. Actually, the process of PAHs absorption by  plant roots is considerably complex and little information about mechanisms for PAHs uptake by the plant roots addressed is available.
Kvesitadze et al. [32] have reported that roots absorb environmental contaminants in two phases: in the first fast phase, substances diffuse from the surrounding medium into the root; in the second they gradually distribute and accumulate in the tissues. In this study, the time course of PHE uptake within 36 h can be divided into two parts ( Figure 1): a fast influx period (the initial to 2 h) and a slow influx period (2 to 36 h). During the fast period, sorption by root cell wall, diffusion and transpiration flow cause a high rate of PHE uptake. Gao et al. [33] have also found an initial rapid uptake phase of PHE by ryegrass. Nevertheless, the low rate of PHE uptake after the initial rapid uptake phase is mainly attributable to passage into root cytoplasm and vacuole. The time course of PHE uptake by wheat roots is consistent with that of glyphosate uptake by suspension-cultured potato [34]. Denis and Delrot [35], and Tilquin et al. [36] have observed that glyphosate uptake by broad bean and Catharanthus roseus cells is an active process. This seems to imply that the active process is involved in PHE uptake by wheat roots.
To further understand the uptake process for PHE in wheat roots, the investigations with respect to the effects of temperature and inhibitor were conducted. PHE uptake by plant roots is related to its concentration [37]. In the concentration range of PHE studied, PHE uptake is best explained by a single saturable system with a K m of 23.7 μM and a V max of 208 nmol g -1 fresh weight h -1 (Figure 2). Denis and Delrot [35] have found that phosphate uptake by broad bean is characterized by a saturating nature. Hart et al. [38] have also observed the similar characteristic in paraquat uptake by roots of intact maize seedlings. They attributed the saturable kinetics to uptake via a carrier-mediated process due to the kinetics of ion or molecule transport through membranes of plant cells like the relationship between an enzyme and its substrate, using terms of enzymology. Our result is in agreement with those reported by Denis and Delrot [35], and Hart et al. [38], which indicates that the carrier-mediated process exists during PHE uptake by wheat roots. Naphthalene is a PAH with two benzene rings, and phenanthrene consists of three condensed benzene rings. The competitive effect of PHE and NAP upon root uptake may appear on account of their similar physico-chemical properties ( Figure 5). Reciprocal inhibition in root uptake between PHE and NAP further suggests that PAH uptake by wheat roots proceeds with a carrier-mediated system, and NAP and PHE share a common transport mechanism.
The fact that uptake is not affected by temperature is an indicator that the compounds are retained by physical sorption rather than biochemically [16] and metabolically coupled membrane transport processes may be inhibited by low temperature [39], whereas physical processes such as adsorption and diffusion are only slightly affected by temperature [15,39]. Moreover, Hart et al. [40] have pointed out that the difference in ion levels measured in intact roots at 23°C and in intact roots incubated at low temperature (2°C) can represent ion taken up across the root plasma membrane. The results presented in Figure 3 display that the rate of PHE absorption at 4°C was a part of the rate at 25°C. The reduction in absorption of PHE by low temperature increased with increasing external PHE concentration in hydroponic solution. A similar effect of low temperature upon Zn 2+ uptake was found previously for sugarcane leaves [27], barley roots [41] and wheat roots [40,42]. In addition, Liang et al. [43] also found Si uptake by cucumber roots was inhibited by low temperature. The above authors interpreted these findings as evidence of the metabolic control of absorption. In the present study, we found that lower temperature of 4°C didn't result in a distinct decrease of PHE apparent solubility in Hoagland nutrient solution containing 0.1% Hoagland nutrient solution (pH 5.5) and Millipore water (pH 5.5) were employed for hydroponic solution. a, b, c, p < 0.05. PHE, phenanthrene. pH 1 , pH initial . pH 2 , pH after 4 h . pH = pH after 4 h -pH initial .
methanol as compared to the temperature at 25°C. Therefore, the reduction in phenanthrene uptake by wheat root at low temperature is not due to the decrease in phenanthrene apparent solubility caused by low temperature. Figure 4 shows that 2,4-DNP may inhibit PHE uptake by wheat roots and the inhibition effect of 2,4-DNP on PHE uptake is gradually strengthened with increasing external PHE concentration in the culture solution. The inhibitions by 2,4-DNP and by low temperature are approximately even, with inhibition rate up to almost 40%. The data from Figure 3 and 4 suggest the existence of metabolic mediation in the PHE absorption process. PHE uptake by wheat roots results in an increase in external solution pH (Table 1). Furthermore, the larger the amount of PHE taken up by wheat roots, the higher external solution pH. However, the passive absorption process cannot satisfactorily explain the phenomenon that occurs during the absorption of PHE (a non-ionic and hydrophobic organic compound). It is well known that the NO 3 -/H + symport can cause an increase in external solution pH [15]. Williams et al. [44] and Noiraud et al. [45] have reported that sucrose transport across the plasma membrane is a sucrose-H + symport process. Similar to sucrose, PHE is present in solution as a form of molecule. We presume that PHE influx may be coupled with H + influx and that PHE transport across the plasma membrane proceeds via a PHE/nH + symport mechanism. Although the hypothesis of PHE/nH + symport remains to be further tested, the change in external solution pH during root uptake of PHE indicates that active absorption is involved in PHE influx into wheat roots.

Conclusions
The results obtained in this study show that two biochemical mechanisms interplay in the root uptake of PHE: (i) a fast partitioning, nonmetabolic, and 'downhill' process driven by sorption, diffusion or mass flow, which takes place immediately after the transfer of wheat roots into culture solution, and (ii) a later, slow component, which is mediated by an transporter and metabolism. In the slow process, the competition between PHE and NAP during uptake exists. This study demonstrates that a carrier-mediated, energy-consuming process is involved in PHE uptake by roots of intact wheat seedlings. This information may be beneficial to govern crop contamination by PAHs, and to yield safe produce. It is also useful in remedying soils or waters polluted by PAHs with plants.

Chemicals
Phenanthrene and naphthalene were purchased from Fluka Chemical Corporation with a purity >97%. Their molecular weights are 178.2 and 128.2 g mol -1 , water solubilities at 25°C are 7.3 and 247.3 μmol L -1 [26].

Plant preparation and growth conditions
Wheat (Triticum acstivnm L.) seeds germinated on moist filter paper for 4 d at 25°C in the dark after surface sterilization in 10% H 2 O 2 for 10 min and thorough rinse with Millipore (Milli-Q, Billerica, MA, USA) water. The nine uniform-sized seedlings were transplanted per 600-mL beaker wrapped with black plastic and containing 500 mL half-strength aerated Hoagland nutrient solution for 5 d and then transferred to the full-strength Hoagland solution for 5 d. The nutrient solution prepared with Millipore water and the initial pH of the solution was adjusted to 5.5. Wheat seedlings were grown in a climate chamber under controlled conditions (photoperiod 16 h light/8 h dark; light intensity 400 μmol m -2 s -1 ; day/night temperature of 25/20°C; relative humidity 60%). After a 10-d growth in Hoagland nutrient solution, the wheat seedlings were immersed in Millipore water for 24 h and then employed in the subsequent phenanthrene uptake experiments.

Time course of root uptake of phenanthrene
Nine intact 14-d-old wheat seedlings were immersed with their roots in a 600-mL beaker containing 500 mL aerated complete Hoagland nutrient solution (pH 5.5) with 5.62 μM (1.0 mg L -1 ) phenanthrene and 0.1% methanol. Seedlings were allowed to accumulate phenanthrene at 25°C for 2, 4, 8, 16 or 36 h. At each sampling point, three beakers were removed for analyzing the phenanthrene uptake by wheat roots.

Concentration-dependent uptake of phenanthrene
As above, batches of intact wheat seedlings were transferred to 600-mL beakers containing 500 mL fullstrength Hoagland nutrient solution (pH 5.5) with 0.1% methanol. The uptake of phenanthrene was detected after 4 h of uptake in the solutions with phenanthrene at concentrations of 0, 1.12, 2.25, 3.37, 4.49, 5.62 and 6.74 μM. The concentration-dependent uptake experiment was performed at 4°C and 25°C. In order to keep low temperature (4°C), ice-bath was employed [39,43]. There are triplicates in each treatment. Transpiration was measured gravimetrically using plant-free pots as controls, and the difference between 4°C and 25°C in transpiration was negligible within 4 h. Under the experiment conditions, the difference in phenanthrene apparent solubility between 4°C and 25°C was trifling.

Phenanthrene uptake by wheat roots with or without 2,4dinitrophenol
This experiment was conducted at 25°C. The procedures were the same as those in concentration-dependent uptake of phenanthrene. The concentration of 2,4-dinitrophenol in Hoagland nutrient solution was 1 mM [27,43].

Uptake competition between phenanthrene and naphthalene
In uptake competition experiment, there were three treatments: a) addition of naphthalene at a concentration of 3.91 μM (0.5 mg L -1 ); b) addition of phenanthrene at 2.81 μM (0.5 mg L -1 ); and c) addition of naphthalene and phenanthrene at 3.91 and 2.81 μM. The experiment was carried out as described in concentration-dependent uptake of phenanthrene.

Change in pH during phenanhtrene uptake
Hoagland nutrient solution and Millipore water were employed to test the change in pH during uptake of phenanthrene. Wheat roots were exposed to three phenanthrene levels, 0, 2.81 and 5.62 μM, with each treatment being replicated three times. The procedures were the same as uptake competition experiment. After 4 h of uptake, pH values of Hoagland nutrient solution and Millipore water were measured with a pH meter.

Extraction and analysis of phenanthrene and naphthalene
After harvest, wheat roots were rinsed with methanol for about 10 seconds, and then washed with sufficient Millipore water to remove the phenanthrene and naphthalene on root surface, followed by wiping with tissue paper [46,47]. Wheat roots and shoots were weighed and ground in a glass homogenizer. Homogenized tissue samples were extracted in acetone/hexane (1:1, v/v) mixture by ultrasonication repeated three times (30 min each time). The combined solvent extracts were passed through an anhydrous Na 2 SO 4 column with elution of the 1:1 mixture solvent of acetone and hexane. The eluents were then evaporated to dryness at 35°C in a rotary evaporator and dissolved in 12 mL hexane. Subsequently, the 12-mL solvent was cleaned in a 2-g silica gel column and eluted with 25 mL hexane/dichloromethane (1:1, v/v) solvents. The eluents were evaporated to dryness again and exchanged to 2 mL methanol. Prior to analysis of phenanthrene and naphthalene by high performance liquid chromatography (HPLC) with ultraviolet (UV) and fluorescence detection, all extracts were filtered with 0.22 μm filter units [4,48]. The mean recoveries of phenanhtrene and naphthalene acquired by spiking wheat samples with standards were 97% and 86%, respectively, for the entire procedure. None of the data reported here have been corrected for recovery. PAH contents in wheat tissues and pH values in Hoagland nutrient solution were subjected to variance analysis and statistically compared in the light of the Duncan's test at the 0.05 probability level. The inhibitions of low temperature and DNP were statistically compared according to the paired t-test at 95% confidence level.
The HPLC system employed consisted of an automatic injector (Waters 717), a binary high-pressure pump (Waters 1525), a UV detector (Waters 2487), and a fluorescence detector (Waters 2475). Separations were performed with a reverse phase Symmetry C 18 (ø 4.6 × 150 mm, 5 μm particle) column. The temperature of the HPLC column was kept constant at 30°C. The used mobile phase was methanol and Millipore water (80:20, v/v), with a flow rate of 1 mL min -1 . The injection volume was 10 μL. Phenanthrene and naphthalene were quantified at 293.5/395 (excitation/emission wavelength) and 254 nm for fluorescence detector and UV detector, respectively.