Assessment of beneficial fungal microorganism’s bio-efficacy in stimulating morphological and physiological parameters of Allium cepa plants grown in soil amended with fish wastes

Background The increase in the human consumption of fish results in the production of organic fish wastes (FW). For enhanced soil fertility and plant growth at a lower cost and without the negative impacts of chemical fertilizers, these wastes could be employed as a valuable organic fertilizer. To determine the synergistic bio-efficacy of Trichoderma sp. and arbuscular mycorrhizal (AM) fungi in stimulating the morphological and physiological characteristics of FW-fertilized Alium cepa, as well as to investigate their involvement in boosting soil fertility, the current study was carried out. Overall, eight treatments were applied as follows: AM, Trichoderma sp., AM + Trichoderma sp., FW, AM + FW, Trichoderma sp. + FW, AM + Trichoderma sp. + FW, and control. Growth and physiological assessments of onion plants were taken after 8 weeks from FW application. Results Our results showed that FW application combined with AM fungi and Trichoderma sp. inoculations increased aggregate stability of the soil (glomalin content) and soil chitinase activity. Moreover, using the bio-inoculations along with FW amendments significantly (p < 0.05) improved the photosynthetic pigments, protein, carbohydrates, and nutrients content of onion plants. It's interesting to note that the triple interaction of AM + Trichoderma sp. + FW led to the greatest increase in plant height, root length, number of leaves, and leaf area as well as total fresh and dry weights of shoots and roots. Besides, AM fungal colonization was at its highest percentage with Trichoderma sp. inoculation, although this percentage decreased with FW addition. Conclusion We concluded that the combined treatments of AM fungi and Trichoderma sp. along with FW application to the soil can be proposed as a successful strategy for plant performance in nutrient-deficient soils as both fungal inoculants are capable of degrading these wastes and converting them into manure suitable for farming so plants can uptake the minerals effortlessly. Supplementary Information The online version contains supplementary material available at 10.1186/s12870-022-03965-3.

Page 2 of 18 Abdelhameed and Metwally BMC Plant Biology (2022) 22:617 these fertilizers results in increasing soil acidity and quick decay of soil besides groundwater, other than an increase in crop vulnerability to pests and diseases and the disruption of the natural ecosystem [1,2]. Because of the developing awareness of environmental preservation and stability [3], organic fertilizers have become a desirable alternative to chemical fertilizers. These organic fertilizers are helpful in low-soil-health circumstances, such as malnutrition, scarce water-holding capacity, excessive nutrient leaching, and poor root development [4][5][6]. Symbiosis with helpful fungi like arbuscular mycorrhizal (AM) fungi and Trichoderma has been predicted as one of the mechanisms of plant tolerance, stress avoidance, and reduction of chemicals used in agriculture. This will help to improve the quality of these soils and plant species' ability to resist this harsh environment. Onion (Allium cepa L.), being a bulbous vegetable, responds well to organic fertilizers addition. According to Gadelrab and Elamin [7] and Petrovic and Pokluda [8], organic fertilizers were more effective in obtaining a greater yield and quality of onions; these fertilizers improved the chemical and physical quality of onions. Fertilizers based on organic matter mixed with plant growth-promoting microorganisms, e.g. AM fungi, Trichoderma spp. and plant growth-promoting bacteria such as Azosprillum, Pseudomonas and Azotobacter are the most widely used bio additives [9].
Efficiently, these beneficial fungi can mobilize nutrients in the soil from unavailable to available forms through biological processes [10], allowing the microorganisms to colonize the rhizosphere together with the plants and consequently increase the supply or availability of nutrients to the host plants, along with increasing soil fertility and crop production in sustainable farming [11]. On the other hand, the addition of organic amendments to the soil will reverse soil property degradation. Organic amendments' beneficial effects include increased plant nutrient availability, augmented humus content and thereby improved water-holding capacity, better soil structure, besides increased microbiology activity [12]. Also, these organic amendments can improve the soil's physical and chemical characteristics, enabling marginal soils to become more productive [13]. In this respect, the application of organic amendments to the soil before AM fungal inoculation has been suggested [14]. Among different organic amendments, fish waste (FW), is capable of promoting plant growth and augmenting the microorganism activity existing in the soil [5,15]. Jayasundara et al. [16] stated that liquid fertilizer of FW mixed with Tithonia diversifolia had a significant growth enhancement of Abelmoschus esculentus. Moreover, fermented liquid FW showed significantly higher growth performance and significantly increased the leaf area of Solanum melongena [17].
The enhanced growth of plants grown on FW may be attributed to their abundance of chitin, which is nature's second most prevalent polysaccharide after cellulose. Chitin is a polymer of N-acetylglucosamine and is tightly associated with proteins, lipids, pigments, calcium carbonate and extra inorganic compounds [18]. It has a high N content of 6.1%-8.3%, which is a comparable level to other organic fertilizers thus used as N and energy sources by plants and microbes. In addition to providing N, the exoskeletons of crustaceans are also rich in Ca minerals [19].
The use of FW in conjunction with beneficial microbes to discharge nutrients to crops for increased sustainable production has previously been reported [5,20], as these microbes are essential in organic matter breakdown and release complex compounds for plant utilization. Also, chitin present in FW can be hydrolyzed by several chitinolytic enzymes produced by bacteria, fungi, and plants. Fungi are engaged in organic matter decomposition and protein transformation into soluble N nutrients, amino acids and ammonium [21]. Ahmad et al. [22] stated that plants can access N from FW through microbial breakdown and release of inorganic forms of N (NH 4 + and NO 3 − ). Also, Nandhini et al. [20] used Brevibacillus agri, Bacillus cereus, Bacillus licheniformis and Brevibacillus parabrevis for FW breakdown to release amino acids and nutrients to crops. AM fungi, belonging to the phylum Glomeromycota, are a foremost constituent of the soil microbiota and undoubtedly exemplify the most vital terrestrial symbiotic microbes [23][24][25]. Researchers have become interested in these fungi as a result of their capacity to establish close relationships with between 70 and 90% of plant species [26,27] and improve the photosynthesis, growth attributes, and enzyme activities of the plants [24,28]. Trichoderma species are also endophytic saprophytes because they readily colonize the host plant's root surface or cortex and benefit the host plant's health and growth [29][30][31].
These soil fungi are great sources of chitinases and could be exploited in agriculture, biotechnology, and medical applications to catabolize chitinous waste [6,32]. By employing shrimp shellfish debris as a substrate for solid-state cultivation, Rattanakit et al. [33] developed chitinase. The ability to hydrolyze chitin present in FW is a characteristic of fungal genus Trichoderma such as T. virens, T. aureoviride, T. citrinoviride,, T. harzianum as they are capable of producing various polysaccharide degrading enzymes i.e. exochitinases, chitobiosidases and endochitinases [34][35][36]. Furthermore, an induction of specific root chitinase enzymes during AM fungal symbiosis of Glomus mosseae, G. intraradices and Gigaspora rosea has been described in several plants [37]. Moreover, the highest rates of chitinase activity were found in the roots of sorghum and tobacco plants inoculated with AM fungi [6,37].
Furthermore, AM fungi can contribute to soil aggregate stability directly through the physical effect of their network that are formed around soil particles and indirectly via the hyphal exudation of an iron-containing, heat-stable glycoprotein termed glomalin as an aggregate binding agent [38,39]. Glomalin, a widespread AM fungi produced glycoprotein by mycelium and spore walls of AM fungi, is essential for both ecosystem functioning and ecological restoration and plays an important role in structuring soil as in the low nutrient-deficient soils; as the low productivity and fertility of the soil seriously limit plant growth. Besides, glomalin possibly evolved on the fungal hyphae as a defensive coating to avoid the loss of water and nutrients before reaching the plant host and to protect the hyphae from decay. In addition, glomalin helps to stabilize soil aggregates through the creation of a polymer-like protective lattice on the aggregate surface [40][41][42]. Medina and Azcón [12] reported that the organic amendment applications improved the soil quality of degraded soils and the plant's ability to resist these conditions. As a result, the goal of this research was to evaluate the utility of fungal inoculums like AM fungi along with Trichoderma sp. in improving the quality of soils treated with FW and their enhancing capacity for the growth performance and physiological processes of Allium cepa plants. Moreover, this study was conducted to determine whether the addition of FW to the soil can support the bio-fertilizer (AM fungi and Trichoderma sp.) in enhancing the nutrient contents of onion plants.

Identification of Trichoderma sp
In this study, the morphological identification was confirmed by molecular analysis of ITS rDNA sequence (18S-28S rRNA) (Cai and Druzhinina [43] and Druzhinina, [44]) flanking ITS1 (5.8S rRNA), and ITS2 using ITS1/ITS4 primers as Trichoderma sp. The retrieved ITS sequence was deposited in NCBI GenBank under the accession No. ON479613. The sequence data were aligned for their similarity with the other related strains with BLAST program. Molecular evolutionary genetic analysis (MEGA version 7) software was used for phylogenetic analysis [45]. Phylogenetic tree as shown in Supplementary file (Fig. S1) was made using neighborjoining method with 1000 bootstrap replicates based on ITS gene sequences. The closets homologous to the sequences were selected, and multiple sequence alignments were carried out using the ClustalW program in the MEGA7 software. The evolutionary distances were computed using the maximum composite likelihood method.

Mycorrhizal colonization
According to Sutton [46], there are three phases to AM fungal colonization: (1) a lag phase during which spore germination, germ tube growth, and initial penetration occur; (2) a rapid growth phase during which the fungus spreads throughout the roots and develops external mycelia; and (3) a stable phase during which the ratio of colonized roots to non-colonized ones stays relatively constant. Different AM fungal species inoculating onion plants resulted in a significant increase in AM fungal root colonization (F%) (Fig. 1a), which is shown in Fig. 1b, c, and d as various colonization forms [intraradical (IH), vesicles (V), and arbuscules (Arb)], whereas colonization was not seen in onion roots that had not been inoculated with AM. Although, Ellyzatul et al. [15] found that FW application increased the activity of microorganisms present in the soil. Besides, Johansson et al. [47] reported that organic fertilizers addition favors bacterial communities that encourage spore germination and increase the rate of AM fungal colonization. Our findings indicated that the high availability of macro and micronutrients added to the soil through FW, particularly the amount of P [48] that increases the plant photosynthetic rate and increases the amount of sucrose in the plant, caused a decrease in AMF root colonization compared with AM plants [49]. Similar results were reported [6]. Also, Sousa et al. [50] observed a decrease in AM fungal root colonization percentage in cowpea plant roots with manure and gliricidia applications. However, our results showed a significant increase in F% with Trichoderma sp. application in comparison to FW. These findings are in agreement with Metwally [28] who stated synergistic influence of T. viride on AM fungal colonization in onion roots because the effects of the soluble and volatile exudates produced by Trichoderma are complicated [31,51]. Also, Trichoderma released volatile compounds that stimulate the development of AM fungal auxiliary cells [30,52].

Plant growth performance
With the application of FW, there was an improvement in the onion growth traits (Tfwt, TDwt, plant height, root length, leaf number, bulb and neck diameter), although this increase was not significant (Table 1). This result was not consistent with Ranasinghe [3] and Ellyzatul et al. [15] who reported that FW application significantly increased the leaf number, leaf area, shoot length and fruit weight of Albemonchus esculentus and cucumber plants. This enhancement may be attributed to the improvement in soil structure and elevation in the plant growth regulators and levels of plant nutrients as FW is a rich source of proteins, carbohydrates and minerals like P, Zn, N, Ca, K, Mg, and Fe which increased root growth and leaf area of onion plants [53]. As shown in our previous recorded data [5] for macro and micronutrients analysis of FW, results showed that FW is rich in N (7.01%), P (12,201 µg/g), K (1573 µg/g), Ca (89,762.5 µg/g), Mg (189 µg/g), Fe (266.81 µg/g) and Zn (94.37 µg/g). Also, the increase in onion growth attributes due to FW application could be a result of increased phosphatase and chitinase activity [54]. A noteworthy finding was that an additional boost in onion growth parameters like bulb and neck diameter with their triple interaction (AM fungi, Trichoderma sp. and FW application) was appeared through results of three-way ANOVA ( Table 1, Fig. 2) compared to when each fungus was administered separately. These results support Abdelhameed and Metwally's hypothesis [55] that extraradical mycorrhizal hyphae are involved in the mycorrhiza-mediated enhancement of plant nutrition. Moreover, statistical analysis indicated that AM fungal inoculation with FW had the highest statistical significance on both TFwt and TDwt of onion plants, followed by Trichoderma sp. According to Metwally and Al-Amri [30], AM fungus and T. viride dual inoculation improved Fwt and Dwt, plant height, and root length of Allium cepa plants in comparison to the control plant, findings that are consistent with our investigation. Compared to control plants, onion plants dual-inoculated with AM fungi and Trichoderma sp. in FW applied soils improved by 25.7%, 35.5% and 36.1% in plant height, root length, and neck diameter, respectively. The synergistic effect of Trichoderma sp. with AM fungi, which made P, N, K and micronutrients like Fe, Zn, Mn, and Cu from FW more soluble and subsequently enhanced their uptake with AM fungi, may support this. The decomposition of the FW was enhanced by the activity of these two types of microorganisms (AM and Trichoderma sp.) in the soil, which also improved the transfer of organic matter and other nutrients to the soil, supplying more nutrition for the plant's growth [21].

Photosynthetic pigments contents
Precision agriculture has been reported to place a special focus on the importance of leaf chlorophyll concentration as a good indication of photosynthesis and plant productivity [21]. Our results revealed that FW, AM fungi and Trichoderma sp. single inoculation increased the total chlorophyll contents of onion plants by 47.5%,  58.6% and 5.1%; respectively compared with the control (Fig. 3d). These results are matching with the previous studies on cowpea and fenugreek plants [55,56]. Besides, Begum et al. [57] stated that the increase in photosynthetic activities of plants is directly associated with the improved growth frequency of AM fungal inoculation that is directly linked to the N and P uptake (Fig. 4), which move towards roots and stimulate the growth parameters (Fig. 2). Moreover, AM plants sequester more Zn through the means of extraradical mycorrhizal hyphal structure and improve Mg uptake (which are critical constituents necessary for photosynthesis), leading to an increase in chlorophyll concentrations, consequently increasing photosynthate production, stomatal conductance, transpiration rate and carbon assimilation as well as plant progress [24]. Additionally, the rise in chlorophyll content ( Fig. 3a, b and d) with FW application is consistent with Liu et al. [21] and Dalorima et al. [58] in pineapple and watermelon. Similarly, Khandaker et al. [59] reported that fermented fish extract application increased the chlorophyll content of chilli plants. The stimulatory effect of FW on photosynthesis might be due to its richness with Zn and Fe which are required for plant metabolism and chlorophyll biosynthesis. Conversely, Ellyzatul et al. [15] reported that FW application did not affect chlorophyll a, b and carotenoid content of cucumber leaf. Noticeably, the highest value of carotenoids was shown with AM, Trichoderma sp. and FW triple interaction (0.209 ± 0.0095 mg/g Fwt) followed by AM and FW dual treatment (0.139 ± 0.0064 mg/g Fwt), as compared with AM and Trichoderma sp. single inoculation (0.118 ± 0.0054 and 0.095 ± 0.0043 mg/g Fwt); respectively as shown in Fig. 3c. The maximum amplification in onion pigments content (Fig. 3e) dually treated with AM fungi and Trichoderma sp. and applied with FW was in line with the results of Metwally and Al-Amri [30]. These are consistent with Cruz et al. [60] who reported that soil enrichment with organic matter increased the carotenoids content in lettuce leaves.

Total soluble carbohydrates content
The contents of soluble sugar are related to the plant's growth potential and stress-resistance ability [21]. Based on the results of three-way ANOVA, there was a significant increase in total soluble carbohydrates contents with AM, Trichoderma sp. and FW interactions ( Table 2). As a representative in Fig. (4), total soluble carbohydrates in onion plant leaves were increased by 12.9, 16.5 and 1.9% with FW additions, AM and Trichoderma sp. application applied singly which was consistent with the previous results of Metwally and Abdelhameed [27] and Metwally [28] that attributed this increase to the enhancement in photosynthesis that allowed higher sugars distribution from shoots to roots and hydrolysis of starch to soluble sugar in AM plants. Furthermore, the augmentation in soluble carbohydrates as a result of Trichoderma sp. application may be a result of the increase in total chlorophyll content as our results showed (Fig. 3d). This result agreed with Badar and Qureshi [61] findings in the combination of T. hamatum and Vigna mungo-specific Rhizobium. Also, the maximum amplification in soluble carbohydrates content was reported in onion plant leaves dually treated with AM fungi and Trichoderma sp. and applied with FW as clearly apparent in Fig. (4). The increase in total soluble carbohydrates content with FW application was in line with Liu et al. [21] who reported that the composted pineapple markedly augmented the soluble sugars contents. This may be attributed to an increase in the amount of organic matter and an improvement in the physical and chemical aspects; composted residue provides a better soil environment and a plentiful source of carbohydrates and N that promote the growth of beneficial microorganisms [62]. Contrary to our results, Nurbaity et al. [13] stated that the interaction between organic matter such as compost and biochar and AM  fungi had no significant effects on the protein, lipid and carbohydrate contents of potato tubers.

Total soluble protein content
Protein is an essential compound in food, in which its concentration in plants is highly dependent on N availability in the soil at sowing time; N is released during the growing seasons through mineralization of soil organic matter [13]. In general, the tripartite interaction of FW addition, Trichoderma sp., and AM fungal inoculation significantly affected the protein content of onion roots (Table 2). Based on Fig. (4), in comparison to plants that weren't treated, FW amendments caused an increase in the amount of onion root protein in AM and Trichoderma sp. inoculated plants. When onion roots were dual inoculated with AM and Trichoderma sp. and supplemented with FW, its highest level was detected (0.335 ± 0.015 mg/g Fwt) compared to control ones. These are in accordance with results that reported in sorghum plants inoculated with Glomus intraradices and amended with FW which exhibited an increase in total soluble protein [6]. Where, proteins can be either covalently linked within the AM fungal cell wall or they can non-covalently associate with the wall of the plant roots [57]. Also, the vital role of AM fungi in protein stimulation may be attributed to AM fungi facilitating certain plant genes activation and nutrient accumulation [23,27,63,64]. In addition, Trichoderma sp. significantly increased the protein content of the roots in the majority of non-AM and AM plants treated with or without FW. This is consistent with our latest research with Allium cepa plants that were dual-inoculated with AM fungus and Trichoderma sp. and treated with FW [5]. In a similar manner, the seed protein of Vigna sinensis was also shown to be increasingly affected by FW, Pseudomonas bacteria, and their interactions [65]. This increase may be a result of increased biological N fixation, nitrate uptake, and formation of nitrogen oxide converted to the enzyme L-arginine, which is essential for protein biosynthesis [66].

Chitinase enzyme
As a result of the high cost of chitinase as well as expensive pretreatment operations of the crab shell and shrimp, the synthesis of chitin and its hydrolyzed derivatives, such as acetylglucosamine and chitooligosaccharides from fish industry wastes has been limited [15,32,67]. So, using chitinases produced by chitinolytic microbes for effective bioconversion of chitinous waste has become increasingly essential. Microorganisms are regarded as the preferred chitinases source due to their huge abundance in nature and the simple availability of raw materials, resulting in cheaper chitinases manufacturing costs [32]. The soil chitinase enzyme activity was significantly influenced by the three variables (AM, Trichoderma sp. and FW), and AM fungal inoculation had the maximum pronounced effects (Table 2, Fig. 5). Where, soil treated with FW alone or in combination with AM fungi and Trichoderma sp. led to higher chitinase activity than control soil (Fig. 6). Highly significant differences in soil chitinase activity were detected between AM and non-AM onion plants grown in soils treated or untreated with FW. Our results are consistent with Brzezinska et al. [32] reports that shrimp waste was used in agriculture as an economical natural N fertilizer, whereas earlier as [68], Brzezinska et al. documented their decomposition by microorganisms.
The significant increase in chitinase activity in soils applied with AM onion plants was in accordance with Pozo et al. [37] who reported that chitinase activity was higher in AM tomato roots, where their stimulation appears as a specific response to AM fungal symbiosis. Also, these results confirmed the previous report of distinguishing three chitin synthase gene proteins during colonization of Gigaspora margarita by a reverse transcription-polymerase chain reaction [69]. In this regard, Abdel-Fattah and Mohamedin [6] revealed that AM sorghum plants considerably exceeded non-AM plants in terms of soil chitinase activity in both control and chitinamended soils. Likewise, the increase in soil chitinase activity with Trichoderma sp. application was in line with Haran et al. [70] who stated that T. harzianum produces two N-acetylglucosaminidases, four endochitinases, and one chitobiosidase. Also, T. saturnisporum produces Chitobiosidase [71].

Glomalin related soil protein (GRSP)
Glomalin, a glycoprotein at least partially formed by AM fungi, plays an important role in soil structuring. The role of AM fungi in increasing the GRSP content has been demonstrated by several studies [39,41,72]. GRSP, which consists of proteins, carbohydrates, aliphatic components and multivalent cations (i.e. Fe and Al), acts as a glue to bind soil particles [38,39]. Where, cations similar to Fe and Al could encourage compound precipitation and build bridges between particles of clay and organic matter that result in soil aggregation [39,42].    Figure 6 shows that the concentrations of T-GRSP and EE-GRSP in rhizospheric soil of Alium cepa increased with AM, Trichoderma sp. and FW application, varying from 1.082 ± 0.049 mg\g for T-GRSP and 0.263 ± 0.036 mg\g for EE-GRSP as opposed to control one (0.712 ± 0.033 and 0.148 ± 0.0068 mg /g soil respectively). Additionally, the EE-GRSP and T-GRSP in soils were significantly affected by the interactions between the three variables, according to ANOVA results (Table 2, Fig. 5). Although there was a decrease in AM fungal colonization with FW application, there was an increase in GRSP with FW. According to Gryndler et al. [73], humic substances, such as fulvic acids that result from the organic fertilizers decomposition, adsorb free cations from the soil solution and may favor the physiological functions of the fungal mycelia (absorption and transport). Also, improving the soil structure contributes to the production of AM fungal mycelia. Additionally, the results shown in Fig. 6 demonstrated that EE-GRSP and T-GRSP were much greater in the soils of AM-inoculated plants than they were in the soils of non-AM-inoculated plants. This was consistent with Cheng et al. [39] who stated that F. mosseae considerably increased the contents of EE-GRSP and T-GRSP under well-watered and drought stress conditions. Also, Yang et al. [41] suggested the important role of AM fungi in aggregate stability under heavy metals stress conditions. Sousa et al. [50] also observed that when compared to the control, there was a substantial impact of organic fertilization on GRSP content and an increase in the GRSP content was observed in the rhizosphere of maize and cowpea at all fertilization systems. It is probable that the AM fungi augmented the propagules number, which is used as an existence strategy, and favored the increase of glomalin production. Glomalin accumulation in the soil, according to Driver et al. [74] and Meng et al. [75], is due to the decomposition of spores and hyphae and, to a lesser degree, to the passive release or secretion of the hyphae.

Mineral nutrient analysis
Excessive land use might have a dramatic effect on overall biodiversity, which, as shown by many studies, can, in turn, affect the role of the environment [57]. A prominent characteristic of the AM fungal relationship with plants is the transfer of nutrients [76]. AM fungal inoculation can significantly boost the concentration of different macronutrients and micro-nutrients, leading to increased production of photosynthate and thus increased accumulation of biomass [28,77]. The results concerning the effect of FW additions in the presence and absence of AM fungi and Trichoderma sp. application on macronutrients (N, P, K, Ca and Mg) of onion shoots and roots are shown in Fig. (4 and 6). The interactions between FW, AM, and Trichoderma sp. had a significant impact on the nutritional contents of the onion shoot and root, according to the ANOVA results. Our results of increased macronutrients in onion tissues coincide with the latest reports by Metwally [28] and Metwally and Al-Amri [30] of increases in leaf area, and N, K, Ca, and P contents, reflecting enhancement in plant growth. The probable explanation is that Trichoderma works independently to solubilize inorganic minerals and boost plant nutrient absorption [78]. Also, AM fungi produce fungal structures like arbuscules and extra-radical mycelium, which assist and improve in exchange of inorganic minerals and nutrient uptake, ultimately imparting a considerable vigor to host plants [57]. Similarly, FW added to the control soils caused an increase in the nutrient accumulation in both onion shoot and root tissues, because FW is a rich source of minerals like P, Ca, Mg, K, Zn, Fe as well as N (the main source of soil nutrition besides a well-known mineral fertilizer) [53]. One interesting finding was that FW, AM and Trichoderma sp. application results in higher proliferation in onion total P, N and K (64.1, 45.9 and 63.6%) than that of AM (38.7, 23.1 and 30.8%) or Trichoderma sp. single inoculated (17.2, 3.5 and 11.1%); respectively.
Apart from the macronutrients, the trend of the increase was repeated with micronutrients (Zn and Fe), where their contents were significantly increased (p < 0.05) in onion shoots and roots with AM fungi, Trichoderma sp. Besides, with FW additions, further augmentation in their contents was observed (Fig. 7) compared to their individual inoculations. The increased Zn content in onion roots and shoots was in harmony with Colla et al. [79] in lettuce and tomato co-inoculated with Glomus intraradices and T. atroviride. Moreover, Bagheri et al. [80] reported that AM fungal association exhibited high levels of P, K, Zn, and Mn in Pistachio plants. Therefore, in the nutrient-deficient soils, the incorporation of AM and Trichoderma sp. along with FW additions are of great significance for nutrient uptake and enhancing crop performance which may be attributed to improved plant nutritional status (N, P, K, Mg, Ca and Zn) via an increase in root surface.

Conclusion
The suitable management of organic wastes by converting them to valuable resources is of utmost importance. Our results showed a good efficacy of FW in enhancing all agronomic growth parameters of onion plants grown in pots with the aid of AM fungi and Trichoderma sp. bioinoculants. Moreover, leaf chlorophyll content, carotenoids, roots protein, total soluble carbohydrates and minerals nutrient content of onion plant improved with FW. Also, AM and Trichoderma sp. application along with FW amendments improved soil quality (glomalin related soil protein) and increased chitinase enzyme activity in the soil. Owing to their benefits on soil fertility and chitinase enzyme, FW as a compost progresses the condition of agricultural soils and increases growth and other physiological characteristics of onion plants. It can be concluded that FW is promising for enhancing the growth, development and quality of onion under potted conditions and further future research should evaluate these efficiencies strategies under field conditions.

Fish waste (Raw material)
Scales, heads, and fins of tilapia fish as well as shrimp peels were previously gathered from local fish markets close to Zagazig University, Egypt, and were used as the study's experimental materials (FW). These wastes were cleaned before being detergent-washed. These cleaned wastes were dried, crushed, sieved into fine powder, and used.

Fungal inoculants isolation and preparation: Isolation and identification of Trichoderma sp
Trichoderma sp. was isolated using a serial dilution plate approach from the rhizosphere of egg and pepper plants cultivated in the Egyptian soil of El-Sharkia Governorate. Under aseptic circumstances, one mL of suspension from each dilution was placed into sterile Petri plates containing Rose Bengal Medium [81]. After 3 days, Trichoderma colonies were picked and cultivated on potato dextrose agar (PDA) medium. By inoculating the fungal colonies and incubating them at 28 °C for 7 days, pure isolates were obtained. The morphological description of the Trichoderma isolate was verified by comparing macroscopic features on agar plates with those of a standard fungus atlas manual, as well as microscopic characteristics such as macroconidia, microconidia, as well as conidia shape and hyphae arrangement.

Molecular identification of isolated fungus
The pure isolate of Trichoderma sp. was sub-cultured on a PDA medium and grown for 5 days at 30 °C and its genomic DNA was isolated using the CTAB technique [82,83]. In the presence of liquid N 2 , grinding broke down the cell walls of fungal mycelia. The combination was then incubated at 65 °C with the addition of the CTAB extraction buffer before being purified using phenol, chloroform, and isoamyl alcohol (25:24:1). The genomic DNA was precipitated with cold isopropanol and thrice rinsed with cold 70% ethyl alcohol. Finally, 50 μL of sterile distilled water were used to dissolve the DNA.

PCR Amplification and phylogenetic analysis
Using the ITS1 and ITS4 primers, The ITS1 and ITS2 and the inverting 5.8S coding rDNA were amplified [81]. In a total volume of 50 µL, each PCR Before DNA sequencing, the PCR amplicon was resolved using a 8% agarose gel and purified using a specific PCR purification kit (Accu Prep ® PCR DNA Purification Kit, K-3034-1, Bioneer Corporation, South Korea). MacrogenInc, (South Korea) sequenced the purified PCR products. All inter transcribed spacer sequencing work was also performed by MacrogenInc, (South Korea) and was carried out on both strands of the submitted DNA fragments. The purified PCR products were sequenced using an ABI 377 DNA. Auto sequencer (PerkinElmer, Applied Biosystems Div., Waltham, USA) based on the same primers mentioned before.

Isolation and identification of AM fungi
Spores of AM fungi were isolated from rhizospheric soil of El-Sharkia Governorate, Egypt via wet sieving and decanting technique [84]. The spores were then separated and mounted using polyvinyl lactoglycerol alcohol. Approximately 200 g of individual air-dried soil was distributed in 2 L of water in a large jar and the suspension was left intact for 10-15 min. The suspension was then decanted through the stack of sieves 400, 250, 180 and 38 μm (arranged in decreasing order of mesh size from top to bottom), this process was repeated 2-3 times and the residue from each sieve was collected into a small flask with the aid of glass funnel with a little distilled water. The morphology of spores and sporocarps of AM fungi were observed and then their characters were identified by using Manual for identification [85]. The mixture of identified AM fungal spores of Funneliformis mosseae, F. constrictum, Gigaspora margarita and Rhizophagus irregularis together in pots filled with sterilized sandy clay soil were propagated with Sudan grass (Sorghum sudanenses Pers.) roots as an appropriate trap plant for two cycles of 5 months in the greenhouse.

FW application under the greenhouse conditions
The soil utilized in this study was gathered from a field where onions were formerly grown in El-Sharkia Governorate. After sterilizing the soil with 2% formaldehyde, it was left unattended for 25 days for leakage of volatile poisonous substances. Allium cepa L.
(Golden onion, Giza 20) seeds were obtained from the Agricultural Research Center in Giza, Egypt. They were surface disinfected with 2% sodium hypochlorite for 10 min, washed with sterile distilled water afterward, and then propagated in a nursery greenhouse for seedling production and utilized as the test plant. After receiving permission from the Agricultural Research Center in Giza, Egypt, onion seeds were acquired. The soil samples of 2 kg were put into sterilized plastic pots (25 cm diam.) and then used for the growth of onion seedlings (2 seedlings/ pot) after transplanting (After 50 days of sowing) in the greenhouse. One hundred g/ pot enclosing AM fungal colonized Sudan grass roots and soil mixture enclosed spores (AM fungal inocula) were added during transplanting. Trichoderma sp. inoculum was applied via the addition of 25 mL of spore suspension (2 × 10 7 conidia/ml) / pot in the onion rhizosphere. The control pot did not receive any fungal inoculum but only received 25 mL sterilized distilled water and 100 g sterilized soil. Also, FW of 0.75% (w/w) concentration was added during the transplanting of surrounding onion seedlings. As a consequence, the following 8 different treatments were administered to the potted plants in 5 replicates following the design described above:

Plant and soil sampling
Plant samples from all treatments were collected for morphological and physiological assessment 8 weeks after transplanting and applying FW. Besides, samples of rhizospheric soil were taken for glomalin and chitinase enzyme activity.  [86]. Afterwards, the stained roots were detected with an optical microscope and the percentage of root colonization (F %) was determined according to the following equation:

Root length and plant height
Onion roots were carefully washed under tap water to remove soil particles and their lengths were obtained as the mean value of the longest root of each plant from the bulb base. Also, plant height was measured as the topmost height of the main leaf to the onion base bulb. The data was recorded as the average height or length in cm per plant (cm/plant).

Number of leaves
Total leaves of Allium plants were counted from each pot. The reading was recorded as the average number of all leaves of each plant of all pots divided by the number of total plants for each treatment.

Onion leaf area
The leaf area (cm 2 ) of onion leaves was recorded [87] according to the following equation: where L is the length of the leaf and W is the maximum width of the leaf.

Neck and bulb diameter of onion
The neck diameter of the onion plants after 8 weeks of transplanting and FW application was measured at 3 cm above the surface of the soil and the bulb diameter was recorded after uprooting the plant using a thread.

Total fresh and dry weights of shoots and roots
After taking the fresh weight (Fwt), the shoot and root plant samples were dried in an electric oven at 60 °C till constant weight. The total fresh weight (TFwt) and total dry weight (TDwt) of onion shoots and roots were measured and recorded in grams per plant (g/plant).

% AM fungal Colonization =
(The total number of AM colonized segments) (Total number of root segments observed) ×100 Leaf area (cm2) = Lcm x Wcm x 0.785 Total shoot weight represents the weight of both onion leaves and bulb.

Physiological assessments Estimation of photosynthetic pigments content
The Fwt of onion leaves (0.25 g) from all treatments was crushed separately in a mortar using 10 mL of 85% aqueous acetone solution. The homogenate was centrifuged and the absorbance was measured at 663, 644, 452.5 nm using a UV-visible Spectrophotometer, RIGOL (Model Ultra-3660). The following three equations were applied to estimate the content of the pigments of samples in terms of µg/mL according to Metzner et al. [88].
The calculated pigment content was then expressed as mg/g Fwt as follows:

Estimation of total soluble carbohydrates content
Carbohydrates in onion leaves after FW and fungal application were estimated by the phenol sulphuric acid method as described by Dubois et al. [89] after extracting 0.1 g of dried leaves with 2.5 N HCl. The absorbance was read at 490 nm and carbohydrates concentration was calculated in terms of mg/mL using a glucose standard curve and its content was then expressed as mg/g Dwt. as follows:

Assessment of total soluble protein contents in onion roots
Using BSA as standard, the total soluble protein content in onion roots was calculated as mg/g Fwt after separate homogenization of a known onion root Fwt for all treatments (0.5 g) in NaOH (1 N) as well as centrifugation [90]. One mL of solubilized protein was Chl.a = Chl.a µg mL X Extract volume (mL) Fwt of sample g X 1000 mg∕g glucose = g∕mL × Extract volume × Dilution factor Dry weight of sample g × 1000 combined with newly prepared alkaline copper solution for 10 min. Folin-Ciocalteau reagent was then added and left for 30 min. Afterwards, a UV-visible Spectrophotometer measured the optical density at 700 nm.

Colloidal chitin preparation
Colloidal chitin was prepared according to the technique of Reid and Ogrydiziak [91]. Ten grams of milled chitin were suspended in 100 mL of H 3 PO 4 (85%) and stored at 4 °C for 24 h, this mixture was suspended in 1 L of deionized water and washed until the pH was 5. Sodium hydroxide (NaOH) (1 N) was then added to adjust the pH to 7, afterwards centrifuged at 4000 rpm for 20 min. The pelleted chitin was stored at 4 °C. Chitin concentration was calculated by drying to constant weight [92].

Chitinase activity
Chitinase activity was measured in 10 g air dried rhizosphere in a 60 mL glass bottle. The soil was treated with 1.5 mL toluene for 15 min, followed by the addition of 10 mL of 1% (w/v) colloidal chitin. The mixture was shaken and incubated at 37 °C for 18 h. After incubation, 10 mL of water was added to each bottle and 10 mL of the resulting suspension was centrifuged for 20 min at 4000 g. One mL of the clear supernatant was used to determine the amount of N-acetylglucosamine (NAG) released, following the method of Mian et al. [93]. One mL of the clear supernatant was incubated 1 mL of 1% of dinitrosalicylic acid (DNS) for 5 min in a boiling water bath. The NAG production was evaluated by obtaining spectrophotometer absorbance at 535 nm. Chitinase activity was expressed as μmoles of NAG released per hour per gram of soil.

GRSP analyses
As reported by Wright and Upadhyaya [72], the concentrations of easily extractable glomalin related soil protein (EE-GRSP) and total glomalin related soil protein (T-GRSP) have been estimated. In general, T-GRSP was extracted from 1 g of air-dried onion soil with 8 mL of 50 mmol / L citrate solution (pH 7.0) and then autoclaved for 60 min at 121 °C. After centrifugation at 6000 rpm for 10 min, the supernatant was separated. This step was performed several times on the same sample until there was no red-brown color in the solution. The supernatant was collected and stored at 4 °C. EE-GRSP was extracted with 8 mL of 20 mmol/L citrate solution (pH 7) from 1 g of air-dried onion soil sample and then autoclaved (at 121 °C for 30 min). Centrifuging was performed for 10 min at 6000 rpm, and the supernatant was collected and kept at 4 °C. Bradford analysis at 590 nm and BSA as a standard were used to assess T-GRSP or EE-GRSP in the supernatant [94].

Minerals analysis
For assessment of onion minerals nutrient contents, shoots and roots of all treatments were dried separately, sieved through a 0.5 mm sieve and digested with a mixture of sulphuric acid: hydrogen peroxide at the ratio of 2:1 (v/v) [95]. Afterwards, the calcium (Ca), potassium (K), magnesium (Mg), zinc (Zn) and iron (Fe) contents were determined spectrophotometrically in the digested samples in the Faculty of Agriculture Central lab, Zagazig University by using Inductively Coupled Plasma Spectrometry (Ultima 2 JY Plasma). Total phosphorus (P) of shoot and root was determined at 620 nm [96]. Moreover, total nitrogen (N) of shoot and root dried samples was measured using the Kjeldahl method [97] after digestion with concentrated sulfuric acid (98.8%) and digestion mixture.

Statistical analysis
Using SPSS software version 15 (SPSS, Richmond VA, USA), the results were expressed as the mean of 5 replicates ± standard deviation (SD), and the means were compared for statistical significance by one way ANOVA (significance level (p < 0.05), the confidence interval was 95%, before applying post hoc procedure. Additionally, three-way ANOVA was applied to scrutinize the effects of AM fungi, Trichoderma sp. inoculation, FW addition and their interactions on the total biomass, chlorophyll, carbohydrates and chitinase enzyme activities. The Origin 2017 programme for graphing and data analysis was used to create the figures.
Additional file 1: Figure S1. The phylogenetic relationship between Trichoderma sp. (Trichoderma viride strain RA1, ON479613) showing the ITS sequences of closely related fungal strains retrieved from NCBI GenBank. The percentages of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) are shown next to the branches. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed using the maximum composite likelihood method and are in the units of the number of base substitutions per site. Evolutionary analyses were conducted in MEGA7.