Articles

Early PCD events in male gametophyte development of isopropyl-N-phenyl carbamate resistant Nicotiana sylvestrismutant lines

Authors: Alla I Yemets, Oksana A Stelmakh, Elena A Kravets and Yaroslav B Blume

Nocodazole provokes an apoptosis in isopropyl-N-phenyl carbamate resistant and sensitive Nicotianalines but in two different ways

Authors: Alla I Yemets, Oksana A Stel'makh and Yaroslav B Blume

Does tubulin phosphorylation correlate with cell death in plant cells?

Authors: Alla Yemets, Yarina Sheremet and Yaroslav B Blume

NO signaling functions in the biotic and abiotic stress responses

Authors: David Wendehenne, Kevin Gould, Olivier Lamotte, Jörg Durner, Elodie Vandelle, David Lecourieux, Cécile Courtois, Laurent Barnavon, Marc Bentéjac and Alain Pugin

Uv induced ds(ss)-DNA damage: optical and electrical recognition

Authors: Alina Veligura, Michael Koehler, Wolfgang Fritzsche, Peter Lytvyn, Alexandr Gorchinskyy and Eugenia Buzaneva

Does NO participate in cytokinin signaling?

Authors: Georgy Romanov, Natalia Rakova and Thomas Schmülling

Plant phenolic metabolites as the free radical scavengers and mutagenesis inhibitors

Authors: Adam Matkowski and Dorota Wołniak

Effect of nitric oxide on concentration of intracellular free Ca²⁺ in transgenic Arabidopsis thalianaplants during oxidative stress

Authors: Ekaterina V Kolesneva, Lyudmila V Dubovskaya and Igor D Volotovski

Involvement of ethylene, oxidative stress and lipid-derived signals in cadmium-induced programmed cell death in tomato suspension cells

Authors: Elena Iakimova, Veneta Kapchina-Toteva, Anke de Jong, Atanas Atanassov and Ernst Woltering

Nitric oxide, cell death and increased taxol recovery

Authors: Don J Durzan

Production of nitric oxide and reactions with plant hemoglobins under hypoxic stress

Authors: Christos Dordas, Brian B Hasinoff, Jean Rivoal and Robert D Hill

Nitric oxide functions in the plant hypersensitive disease resistance response

Authors: Matteo De Stefano, Alberto Ferrarini and Massimo Delledonne

Use of RAPD assay for the detection of mutation changes in plant DNA induced by UV-B and γ-rays

Authors: Oksana Danylchenko and Boris Sorochinsky

Biomarker metabolites capturing the metabolite variance present in a rice plant developmental period

This study analyzes metabolomic data from a rice tillering (branching) developmental profile to define a set of biomarker metabolites that reliably captures the metabolite variance of this plant developmental ...

Authors: Lee Tarpley, Anthony L Duran, Tesfamichael H Kebrom and Lloyd W Sumner

The FtsH protease is required for the repair of Photosystem II in the cyanbacterium Synechocystis6803 damaged UV-B radiation

Authors: Otilia Cheregi, Cosmin Sicora, Peter B Kos, Peter J Nixon and Imre Vass

Ambient levels of UV radiation

Authors: Mario Blumthaler

UV-A induced fluorescence images in sun and shade leaves

Authors: Fatbardha Babani, Gabriele Langsdorf, Martin Knapp, Claus Buschmann and Hartmut K Lichtenthaler

Influence of UV-radiation on the photosynthesis and photosynthetic carbon metabolism in high mountainous plants

Authors: Abdumanon Abdullaev, B Djumaev and Kh Kh Karimov

Light-induced morphological alteration in anthocyanin-accumulating vacuoles of maize cells

Plant pigmentation is affected by a variety of factors. Light, an important plant developmental signal, influences the accumulation of anthocyanins primarily through the activation of the transcription factors...

Authors: Niloufer G Irani and Erich Grotewold

Molecular phylogeny and evolution of alcohol dehydrogenase (Adh) genes in legumes

Nuclear genes determine the vast range of phenotypes that are responsible for the adaptive abilities of organisms in nature. Nevertheless, the evolutionary processes that generate the structures and functions ...

Authors: Tatsuya Fukuda, Jun Yokoyama, Toru Nakamura, In-Ja Song, Takuro Ito, Toshinori Ochiai, Akira Kanno, Toshiaki Kameya and Masayuki Maki

Floral gene resources from basal angiosperms for comparative genomics research

The Floral Genome Project was initiated to bridge the genomic gap between the most broadly studied plant model systems. Arabidopsis and rice, although now completely sequenced and under intensive comparative geno...

Authors: Victor A Albert, Douglas E Soltis, John E Carlson, William G Farmerie, P Kerr Wall, Daniel C Ilut, Teri M Solow, Lukas A Mueller, Lena L Landherr, Yi Hu, Matyas Buzgo, Sangtae Kim, Mi-Jeong Yoo, Michael W Frohlich, Rafael Perl-Treves, Scott E Schlarbaum…

Imaging plant cell death: GFP-Nit1 aggregation marks an early step of wound and herbicide induced cell death

A great deal is known about the morphological endpoints of plant cell death, but relatively little is known about its sequence of events and / or its execution at the biochemical level. Live cell imaging using...

Authors: Sean R Cutler and Chris R Somerville

Developmental changes in abundance of the VSPβ protein following nuclear transformation of maize with the Soybean vspβ cDNA

Developing monocots that accumulate more vegetative tissue protein is one strategy for improving nitrogen-sequestration and nutritive value of forage and silage crops. In soybeans (a dicotyledonous legume), the v...

Authors: Magali F Grando, Rex L Smith, Cristina Moreira, Brian T Scully and Robert G Shatters Jr

The Arabidopsis Mei2 homologue AML1 binds AtRaptor1B, the plant homologue of a major regulator of eukaryotic cell growth

TOR, the target of the antibiotic rapamycin in both yeast and mammalian cells, is a potent cell growth regulator in all eukaryotes. It acts through the phosphorylation of downstream effectors that are recruited t...

Authors: Garrett H Anderson and Maureen R Hanson

Expression of human dopamine receptor in potato (Solanum tuberosum) results in altered tuber carbon metabolism

Even though the catecholamines (dopamine, norepinephrine and epinephrine) have been detected in plants their role is poorly documented. Correlations between norepinephrine, soluble sugars and starch concentrat...

Authors: Aleksandra Skirycz, Anna Świędrych and Jan Szopa

An evaluation of the effects of exogenous ethephon, an ethylene releasing compound, on photosynthesis of mustard (Brassica juncea) cultivars that differ in photosynthetic capacity

The stimulatory effect of CO₂ on ethylene evolution in plants is known, but the extent to which ethylene controls photosynthesis is not clear. Studies on the effects of ethylene on CO₂ metabolism have shown confl...

Authors: NA Khan

Molecular characterization and functional expression of flavonol 6-hydroxylase

Flavonoids, one of the major groups of secondary metabolites, play important roles in the physiology, ecology and defence of plants. Their wide range of activities is the result of their structural diversity t...

Authors: Dominique Anzellotti and Ragaï K Ibrahim

Use of a highly sensitive two-dimensional luminescence imaging system to monitor endogenous bioluminescence in plant leaves

All living organisms emit spontaneous low-level bioluminescence, which can be increased in response to stress. Methods for imaging this ultra-weak luminescence have previously been limited by the sensitivity o...

Authors: Michel Flor-Henry, Tulene C McCabe, Guy L de Bruxelles and Michael R Roberts

Molecular cloning and functional expression of geranylgeranyl pyrophosphate synthase from Coleus forskohliiBriq

Isopentenyl diphosphate (IPP), a common biosynthetic precursor to the labdane diterpene forskolin, has been biosynthesised via a non-mevalonate pathway. Geranylgeranyl diphosphate (GGPP) synthase is an importa...

Authors: Surang Engprasert, Futoshi Taura, Makoto Kawamukai and Yukihiro Shoyama

Regulation of membrane fatty acid composition by temperature in mutants of Arabidopsis with alterations in membrane lipid composition

A wide range of cellular responses occur when plants are exposed to elevated temperature, including adjustments in the unsaturation level of membrane fatty acids. Although membrane bound desaturase enzymes med...

Authors: Deane L Falcone, Joseph P Ogas and Chris R Somerville

Long-distance transport of L-ascorbic acid in potato

Following on from recent advances in plant AsA biosynthesis there is increasing interest in elucidating the factors contributing to the L-ascorbic acid (AsA) content of edible crops. One main objective is to esta...

Authors: Luigi Tedone, Robert D Hancock, Salvatore Alberino, Sophie Haupt and Roberto Viola

Cloning and expression analysis of cDNAs corresponding to genes activated in cucumber showing systemic acquired resistance after BTH treatment

Infection of plants by necrotizing pathogens can lead to the rapid and localized induction of a complex set of defense responses resulting in a restriction of pathogen growth and spread. Subsequently, an incre...

Authors: Catherine Bovie, Marc Ongena, Philippe Thonart and Jacques Dommes

Validating internal controls for quantitative plant gene expression studies

Real-time reverse transcription PCR (RT-PCR) has greatly improved the ease and sensitivity of quantitative gene expression studies. However, accurate measurement of gene expression with this method relies on t...

Authors: Amy M Brunner, Igor A Yakovlev and Steven H Strauss

Assessment of genetic diversity in Trigonella foenum-graecum and Trigonella caeruleausing ISSR and RAPD markers

Various species of genus Trigonella are important from medical and culinary aspect. Among these, Trigonella foenum-graecum is commonly grown as a vegetable. This anti-diabetic herb can lower blood glucose and cho...

Authors: Rakhee S Dangi, Meena D Lagu, Lal B Choudhary, Prabhakar K Ranjekar and Vidya S Gupta

Discovery of induced point mutations in maize genes by TILLING

Going from a gene sequence to its function in the context of a whole organism requires a strategy for targeting mutations, referred to as reverse genetics. Reverse genetics is highly desirable in the modern ge...

Authors: Bradley J Till, Steven H Reynolds, Clifford Weil, Nathan Springer, Chris Burtner, Kim Young, Elisabeth Bowers, Christine A Codomo, Linda C Enns, Anthony R Odden, Elizabeth A Greene, Luca Comai and Steven Henikoff

Genetic diversity of peanut (Arachis hypogaeaL.) and its wild relatives based on the analysis of hypervariable regions of the genome

The genus Arachis is native to a region that includes Central Brazil and neighboring countries. Little is known about the genetic variability of the Brazilian cultivated peanut (Arachis hypogaea, genome AABB) ger...

Authors: Marcio de Carvalho Moretzsohn, Mark S Hopkins, Sharon E Mitchell, Stephen Kresovich, Jose Francisco Montenegro Valls and Marcio Elias Ferreira

The roles of segmental and tandem gene duplication in the evolution of large gene families in Arabidopsis thaliana

Most genes in Arabidopsis thaliana are members of gene families. How do the members of gene families arise, and how are gene family copy numbers maintained? Some gene families may evolve primarily through tandem ...

Authors: Steven B Cannon, Arvind Mitra, Andrew Baumgarten, Nevin D Young and Georgiana May

Development of microsatellite markers from an enriched genomic library for genetic analysis of melon (Cucumis meloL.)

Despite the great advances in genomic technology observed in several crop species, the availability of molecular tools such as microsatellite markers has been limited in melon (Cucumis melo L.) and cucurbit speci...

Authors: Patricia Silva Ritschel, Tulio Cesar de Lima Lins, Rodrigo Lourenço Tristan, Gláucia Salles Cortopassi Buso, José Amauri Buso and Márcio Elias Ferreira

Antimycin A treatment decreases respiratory internal rotenone-insensitive NADH oxidation capacity in potato leaves

The plant respiratory chain contains several energy-dissipating enzymes, these being type II NAD(P)H dehydrogenases and the alternative oxidase, not present in mammals. The physiological functions of type II N...

Authors: Daniela A Geisler, Fredrik I Johansson, Å Staffan Svensson and Allan G Rasmusson

Incorporation of mammalian actin into microfilaments in plant cell nucleus

Actin is an ancient molecule that shows more than 90% amino acid homology between mammalian and plant actins. The regions of the actin molecule that are involved in F-actin assembly are largely conserved, and ...

Authors: Heiti Paves and Erkki Truve

A systemic gene silencing method suitable for high throughput, reverse genetic analyses of gene function in fern gametophytes

Ceratopteris richardii is a useful experimental system for studying gametophyte development and sexual reproduction in plants. However, few tools for cloning mutant genes or disrupting gene function exist for thi...

Authors: George Rutherford, Milos Tanurdzic, Mitsuyasu Hasebe and Jo Ann Banks

Synthesis of chlorophyll b: Localization of chlorophyllide aoxygenase and discovery of a stable radical in the catalytic subunit

Assembly of stable light-harvesting complexes (LHCs) in the chloroplast of green algae and plants requires synthesis of chlorophyll (Chl) b, a reaction that involves oxygenation of the 7-methyl group of Chl a to ...

Authors: Laura L Eggink, Russell LoBrutto, Daniel C Brune, Judy Brusslan, Akihiro Yamasato, Ayumi Tanaka and J Kenneth Hoober

Cadmium uptake and translocation in seedlings of near isogenic lines of durum wheat that differ in grain cadmium accumulation

Cadmium (Cd) concentrations in durum wheat (Triticum turgidum L. var durum) grain grown in North American prairie soils often exceed proposed international trade standards. To understand the physiological process...

Authors: Neil S Harris and Gregory J Taylor

Comparison of ESTs from juvenile and adult phases of the giant unicellular green alga Acetabularia acetabulum

Acetabularia acetabulum is a giant unicellular green alga whose size and complex life cycle make it an attractive model for understanding morphogenesis and subcellular compartmentalization. The life cycle of this...

Authors: Isabelle M Henry, Mark D Wilkinson, J Marcela Hernandez, Zsuzsanna Schwarz-Sommer, Erich Grotewold and Dina F Mandoli

In vivoanalysis of interactions between GFP-labeled microfilaments and plastid stromules

Plastid stromules are stroma-filled tubules that extend from the surface of plastids in higher plants and allow the exchange of protein molecules between plastids. These structures are highly dynamic; stromule...

Authors: Ernest Y Kwok and Maureen R Hanson

Production of Se-methylselenocysteine in transgenic plants expressing selenocysteine methyltransferase

It has become increasingly evident that dietary Se plays a significant role in reducing the incidence of lung, colorectal and prostate cancer in humans. Different forms of Se vary in their chemopreventative ef...

Authors: Danielle R Ellis, Thomas G Sors, Dennis G Brunk, Carrie Albrecht, Cindy Orser, Brett Lahner, Karl V Wood, Hugh H Harris, Ingrid J Pickering and David E Salt

Sub-cellular trafficking of phytochemicals explored using auto-fluorescent compounds in maize cells

Little is known regarding the trafficking mechanisms of small molecules within plant cells. It remains to be established whether phytochemicals are transported by pathways similar to those used by proteins, or...

Authors: Yakang Lin, Niloufer G Irani and Erich Grotewold

Construction of two genetic linkage maps in cultivated tetraploid alfalfa (Medicago sativa) using microsatellite and AFLP markers

Alfalfa (Medicago sativa) is a major forage crop. The genetic progress is slow in this legume species because of its autotetraploidy and allogamy. The genetic structure of this species makes the construction of g...

Authors: Bernadette Julier, Sandrine Flajoulot, Philippe Barre, Gaëlle Cardinet, Sylvain Santoni, Thierry Huguet and Christian Huyghe

Fusion to GFP blocks intercellular trafficking of the sucrose transporter SUT1 leading to accumulation in companion cells

Plant phloem consists of an interdependent cell pair, the sieve element / companion cell complex. Sucrose transporters are localized to enucleate sieve elements (SE), despite being transcribed in companion cel...

Authors: Sylvie Lalonde, Andreas Weise, Rama Panford Walsh, John M Ward and Wolf B Frommer

Synthesis of L-ascorbic acid in the phloem

Although plants are the main source of vitamin C in the human diet, we still have a limited understanding of how plants synthesise L-ascorbic acid (AsA) and what regulates its concentration in different plant tis...

Authors: Robert D Hancock, Diane McRae, Sophie Haupt and Roberto Viola