Fig. 5

Drought induces anthocyanin biosynthesis in leaf explants. (A-B) Col-0 seedlings grown for 9 days were used. Whole plant (W), shoot + half root (S + Hr), and shoot only (S) were incubated on B5-agar or -liquid plates for 5 days. (A) Representative images are displayed. Size markers indicate 0.5 cm. (B) Measurement of anthocyanin content. Three biological replicates were averaged. Letters indicate groups that are statistically significantly different from each other (P < 0.05, Tukey’s test). Each replicate contains 5–6 explants. Whiskers indicate + SD. (C-E) Leaf explants of the 9-day-old Col-0 seedlings were incubated on B5-agar or -liquid plates for 5 days (C,D) or up to 14 days (E). (C) Representative images are displayed. Size markers indicate 0.5 cm. (D) Anthocyanin content of leaf explants. Six biological replicates were averaged and statistically analyzed using Student’s t-test (*P < 0.05; difference from B5-agar). Each replicate contains 5–6 explants. Whiskers indicate + SD. (E) Rooting rate of leaf explants incubated on agar and liquid medium. Three biological replicates were averaged and statistically analyzed using Student’s t-test (*P < 0.05; difference from B5-agar). Each replicate contains 20–25 explants. Whiskers indicate ± SD