Fig. 1
From: Ethylene signals modulate the survival of Arabidopsis leaf explants
Accumulation of anthocyanins at distant regions from the wound site in leaf explants. (A-B) Col-0 seedlings grown for 9 days were used. (A) Phenotype of the leaf explants during tissue culture. Leaves excised at the indicated site (blade, blade-petiole junction, and petiole) were incubated on B5-agar plates for up to 7 days. DAC, days after culture. Size markers indicate 0.25 cm. (B) Measurement of anthocyanin content. Leaves excised at blade were used. Anthocyanin contents at the site near the wound (SNW) and at the site distant from the wound (SDW) were separately analyzed. Three biological replicates were averaged and statistically analyzed using Student’s t-test (*P < 0.05; difference from SNW). Each replicate contains 5–6 explants. Whiskers indicate ± standard deviations (SD)