Fig. 3
From: Optimization of ATAC-seq in wheat seedling roots using INTACT-isolated nuclei
ATAC-enrichment in accessible vs. non-accessible DNA regions using different number of INTACT-nuclei. a Shot of a Chinese Spring (CS) genome browser showing previously published ATAC-seq data from wheat leaf protoplasts (top) [21] and ATAC-seq from fresh root tips generated in this work (bottom). Green rectangles including ATAC peaks #1 and #2 indicate accessible DNA, whereas the pink rectangle indicates non-accessible DNA. b ATAC-enrichment in peaks #1 and #2 relative to the non-accessible pink region was determined by qPCR using libraries prepared from different numbers of INTACT-purified nuclei from the fresh root tissue