Fig. 1
From: Optimization of ATAC-seq in wheat seedling roots using INTACT-isolated nuclei
Development of INTACT system in tetraploid wheat Kronos. a Constructs used to generate transgenic plants expressing E. coli biotin ligase (BirA) gene and nuclear targeting fusion protein (NTF). The chimeric NTF protein consists of the WPP domain of wheat RanGAP1 for nuclear envelope targeting, RFP for visualization and the biotin ligase recognition peptide (BLRP), for biotinylation by BirA (full sequence in Fig. S1A). b RFP signal around nuclei in the roots of Kronos plants transformed with the NTF vector. Scale 100 µm. c. Western blot of proteins extracted from NTF and BirA lines and from their F1 hybrid. The biotinylated NTF protein was detected in the F1 hybrid but not in the individual transgenic plants (full gel in Fig. S1B)