Fig. 5

Detection of differential looping events during cold stress. A Hi-C contact matrix (2-kb resolution) depicting normalized contact frequencies for normal (lower left) and cold-treated (upper right) Bd21. The chromatin loop is highlighted by red boxes. B APA plots showing aggregated signals across all loops in normal and cold-treated Bd21. The APA value is displayed on the top left. C Heatmaps and average profiles showing the normalized DNase-seq tag density located within 50 kb upstream and downstream of the centre of the loop anchors in normal and cold-treated Bd21. D APA plots of loops lost or gained during cold stress. Individual plots for each condition are shown. The APA value is displayed on the top left. E Distance distributions of the lost, gained, and common loops. F The proportion of differentially expressed genes whose promoters overlapped with the anchors of lost or gained loops during cold stress. The overall prevalence of DEGs is the control group (background)