Fig. 2

The A/B compartment switches and compartmentalization strength decreases during cold stress in Bd21. A The proportions and lengths of A/B compartments in the Bd21 genome. B Comparison of PC1 values obtained from the Hi-C data for normal and cold-treated Bd21. A positive value indicates the A compartment, and a negative value indicates the B compartment. The Wilcoxon test was used to analyse statistical significance. ‘**’ represents a P value < 0.01. C Saddle plot of Hi-C data. Saddle plots were constructed using the FAN-C compartments function. Interactions between A compartments are in the bottom left, and interactions between B compartments are in the top right. The numbers at the centre of the heatmaps indicate compartment strength, calculated as: the natural logarithm of (AA * BB) / AB^2. D The percentage of stable (A-to-A or B-to-B) or switched compartments (A-to-B or B-to-A) between normal and cold-treated Bd21 in the genome. E The percentage of DEGs in stable (A-to-A or B-to-B) or switched (A-to-B or B-to-A) compartments. F Boxplots showing PC1 values of compartments containing different classes of genes under both normal and cold stress conditions. A positive value indicates the A compartment, and a negative value indicates the B compartment