Table 1 The primers used for validation of RNA-Seq by qRT-PCR
From: Transcriptome profiling of barley in response to mineral and organic fertilizers
Accession Number | Primer Sequence (5´- 3´) | PCR Product (bp) |
|---|---|---|
HORVU5Hr1G114130.1 | F: CGTCCCACCATTACCACACATC | 129 |
R: ACACCATCGGCTTCTTCCATC | ||
HORVU2Hr1G119470.1 | F: ACGGCAAGGGAGGAGAGC | 79 |
R: CTTCTCCCCCTCCCTTGCT | ||
HORVU6Hr1G017460.1 | F: CCTCCGCCTGATCTCCATCTAT | 92 |
R: TCGTATGCATGCTTCCTTCGTC | ||
HORVU2Hr1G124020.1 | F: GGGCTCGGACAAGTGGAT | 110 |
R: AACGGATGGCAAGAGGGG | ||
HORVU2Hr1G001740.1 | F: AACGAGGCATGCAGTGATGG | 93 |
R: GGGTGTGGCGATGATGTAGC | ||
HORVU2Hr1G119420.1 | F: GAGCGCGGCAAGGAGGAG | 84 |
R: CTTGGTCAGGCAGCTCTTGG | ||
HORVU5Hr1G056740.1 | F: CCAACATCAACCCCGCCAAG | 114 |
R: TGTTGGGCGTGGAGAAGTGG | ||
HORVU7Hr1G116330.1 | F: AAGGAATCATGGCCGAATGTG | 114 |
R: ATGGATTGTACACGCATGCTAG | ||
HORVU1Hr1G005460.1 | F: CCGCAAATCCCCGAACAATTCC | 93 |
R: CGACTGTTGTTGGGGTTGGGA | ||
HORVU1Hr1G084510.1 | F: CTCGCTCACGCCGTCACC | 104 |
R: CCGCTCTCACCCCACGTC | ||
HORVU1Hr1G000680.1 | F: GGTGGGTCAATGTGTGCTCG | 103 |
R: GGATGGAACGGACTGCAACAC | ||
HORVU2Hr1G122890.1 | F: CCTCGCCCATGATCTACCAGC | 125 |
R: CTTGGCGATGTACTTGGTGGGG | ||
HORVU5Hr1G056030.1 | F: GTGCTGGCGCTCATCGTGG | 107 |
R: CCCGACGACACGAACCCAG | ||
HORVU1Hr1G059900.1 | F: GCAGCAGAACCTCGCCGAA | 78 |
R: TCGCTGTAACCCTCCTGCC | ||
HORVU7Hr1G097730.1 | F: TATGATCCCAGCGCAAACCC | 102 |
R: CATCTCCTGCCACAACATTTCC | ||
HORVU5Hr1G004510.1 | F: ACCTGCAACGACACAAGAAGC | 115 |
R: CACGCTGGAGATGGTCTTTTGT |