Fig. 7
From: The genome of Candidatus phytoplasma ziziphi provides insights into their biological characteristics
Phylogenetic tree construction, conserved motifs and expression analyses of FtsHs in Ca. P. ziziphi. (A) The phylogenetic tree of FtsHs in JWB and other phytoplasmas (FtsH of E. coli (NP_417645.1) was used as an out-group control). The NJ tree was constructed from the amino acid sequences of FtsHs using MEGA 11 with 1000 bootstrap replicates. (B) Conserved motifs of the FtsHs arranged according to their phylogenetic relationships. Ten conserved motifs were identified using Multiple Em for Motif Elicitation (MEME) and are shown in different colors. (C, D, E) The expression of FtsH genes was detected in diseased and healthy tissues (C) and in JWB plantlets treated with different pH values (D) and tetracycline concentrations (E). The treatments of JWB plantlets were the same as in Fig. 6F and G. The data are mean ± SD of three biological replicates. Error bars represent the standard deviation. Asterisks indicate significant difference at **p < 0.01 (Student’s t-test). Different letters between bars indicate significant differences at p < 0.05 (Duncan’s multiple range test). And n.e., no expression