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Fig. 6 | BMC Plant Biology

Fig. 6

From: The genome of Candidatus phytoplasma ziziphi provides insights into their biological characteristics

Fig. 6

Phylogenetic tree construction, expression and enzymatic activity determination of Mn-SodA in Ca. P. ziziphi. (A) Phylogenetic tree and motif analysis of SodA in phytoplasmas (WP_024563569.1 Wheat blue dwarf phytoplasma (Ca. P. tritici); WP_011160792.1 Chrysanthemum yellows phytoplasma (Ca. P. asteris); WP_011412607.1 Aster yellows witches’-broom phytoplasma (Ca. P. asteris); WP_012359262.1 Ca. P. australiense; CAP18217.1 Ca. P. mali.), E. coli (NP_418344.3 Escherichia coli str. K-12 substr. MG1655) and jujube SODs (XP_015877515.1 ZjSOD [Mn], mitochondrial; XP_015874508.1 ZjSOD [Mn] mitochondrial-like; XP_015890462.1 ZjSOD [Fe] 3, chloroplastic; XP_015877712.1 ZjSOD [Fe], chloroplastic-like). (B, C) Morphological phenotypes (a, b, c, d, e, f) and phytoplasmas detected by DAPI (a1, b1, c1, d1, e1, f1) in JWB plantlets under treatments of pH (B) and tetracycline (C). (D, E) The expression of Mn-SodA in JWB plantlets under pH (D) and tetracycline treatments (E). More white fluorescence spots in sieve elements illustrate more phytoplasmas in diseased tissues. JWB plantlets cultured in media with pH 6 were used as controls. TC0, TC50 and TC100 represent JWB plantlets treated with 0 µg/mL, 50 µg/mL and 100 µg/mL tetracycline, respectively. Bar = 2 cm (a, b, c, d, e, f); Bar = 100 μm (a1, b1, c1, d1, e1, f1). Asterisks represents significant differences from the control plantlets (cultured in media with pH 6 (D) or plantlets treated with TC0 (E)) at *p < 0.05, **p < 0.01 (Student’s t-test). (F) SodA expression was detected in diseased jujube leaves. Different letters between bars indicate significant differences at p < 0.05 (Duncan’s multiple range test). (G) The enzyme activity of Mn-SodA in Ca. P. ziziphi by NBT assay. MBP-Tag protein was used as a negative control. n.e., no expression

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