Fig. 6

Comparison of phenotype between VIGS-MeChlD plants and control plants. a qRT-PCR analysis of the expression of MeChlD in the yellow leaves of VIGS-MeChlD plants (MeChlD-1, MeChlD-2 and MeChlD-3). Leaves from VIGS-MeChlD plants were used for each RNA extraction (3 RNA extractions per silencing plants; n = 3). Data indicate means ± SE. Asterisks indicate significantly reduced compared to the control plants (the student’s test, P ≤ 0.05). b The VIGS-induce phenotype in leaves of VIGS-MeChlD plants in cassava at 3 wpi. Bars: 5 cm. c The content of total chlorophyll, chlorophyll a and chlorophyll b were measured in VIGS-MeChlD plants. Data indicate means ± SE. Asterisks indicate significantly reduced compared to the control plants (Kruskal–Wallis test, P  < 0.05). d qRT-PCR analysis of the transcript level of photosynthesis-associated nuclear genes in the yellow leaves of MeChlD-2 plants. Data indicate means ± SE. Asterisks indicate significantly different compared to the control plants (the student’s test, P ≤ 0.05)