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Fig. 5 | BMC Plant Biology

Fig. 5

From: Magnesium chelatase subunit D is not only required for chlorophyll biosynthesis and photosynthesis, but also affecting starch accumulation in Manihot esculenta Crantz

Fig. 5

MeChlD interacts MeChlM and MePrxQ, respectively. a Analysis of the interaction between MeChlD-MeChlM and MeChlD-MePrxQ by the yeast two-hybrid assay. AD represents the pADT7-MeChlM and pADT7-MePrxQ; BD represents pGBKT7-MeChlD and pGBKT7-MeChlM. Interactions were assessed by growth on synthetic defined (SD) dropout medium lacking leucine and tryptophan (Leu/Trp), SD dropout medium lacking leucine, tryptophan and adenine (Leu/Trp/ Ade) and SD dropout medium lacking leucine, tryptophan, histidine and adenine (Leu/Trp/ Ade/His). b Bimolecular fluorescence complementation (BiFC) assays of the interaction between MeChlD-MeChlM and MeChlD-MePrxQ in tobacco leaves. Bright field and fluorescence images were taken and merged. Scale bars: 20 μm

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BMC Plant Biology

ISSN: 1471-2229

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