Fig. 5

Effect of blue and red LED lights on flavonoid content (A), anthocyanin content (B), gene expression of CitF3H (C), and appearance of the juice sacs in blood orange ‘Moro’ in vitro (D). The mRNA levels were analyzed by TaqMan real-time quantitative PCR. 18 S ribosomal RNA was used to normalize the expression of gene in the same conditions. Columns and bars represent the means ± SE (n = 3), respectively. Different letters above each column indicate significant differences at P < 0.05 by Tukey’s HSD test