Fig. 3

Gene expression analysis of CitF3H in the juice sacs of Satsuma mandarin, Ponkan mandarin, and blood orange ‘Moro’ during the ripening process. The mRNA levels were analyzed by TaqMan real-time quantitative PCR. 18 S ribosomal RNA was used to normalize the expression of gene in the same conditions. Columns and bars represent the means ± SE (n = 3), respectively. Tukey’s HSD test (P < 0.05) was used to compare the different varieties. Different letters above each column indicate significant differences of the gene expression of CitF3H among the three citrus varieties in October, December, and February, respectively. Oct: October, Dec: December, Feb: February