Transcriptomic profiling of near-isogenic lines reveals candidate genes for a significant locus conferring metribuzin resistance in wheat

Table 3 Single nucleotide polymorphism (SNP) detected in isolines of NIL pairs within the Qsns.uwa.4 A.2 region

Variant type	Reference seq. variant	Variant (R or S)	Variant physical position (bp)	Associated gene ID	Annotation	Variant position	Overlapping lines
SNP	G	T	709,770,189	TraesCS4A03G1080800	Cellular homeostasis	Within gene (not a DEG)	3S_C and 17S_C
SNP	A	G	714,355,066	TraesCS4A03G1099000 (DEG)	Nitrate excretion transporter (NAXT)	0.10 Mbp from DEG	3S_C, 3S_T, and 17S_C
SNP	T	C	724,909,357	TraesCS4A03G1133400	Biosynthesis of secondary metabolites	Within gene (not a DEG)	3R_C and 17R_C
SNP	A	G	726,024,541	TraesCS4A03G1138500	Glutathione S-transferase	Within gene (not a DEG)	3R_T and 3S_T
SNP	C	T	739,694,493	TraesCS4A03G1181300 (DEG)	Aspartyl protease	0.015 Mbp from DEG	3R_T, 3R_C, and 17R_C

Note: Differentially expressed genes (DEGs) with SNPs common to both NIL pairs are in bold. bp is base pairs. 3R and 17R are resistant isolines, 3 and 17 S are susceptible isolines, C is control, and T is treatment. An SNP at a position marked with bold can be used as a marker for metribuzin resistance breeding

ISSN: 1471-2229