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Table 2 Differentially expressed genes in isolines common to the two near-isogenic line (NIL) pairs on chromosome 4 A and their annotated functions based on RNA sequencing

From: Transcriptomic profiling of near-isogenic lines reveals candidate genes for a significant locus conferring metribuzin resistance in wheat

Genes

log2FC NIL_3

log2FC NIL_17

Gene start (bp)

Gene end (bp)

Gene function/annotation

TraesCS4A03G0812600

( TraesCS4A02G326400 )

–2.96

–3.49

612,401,995

612,403,260

Ethylene-responsive transcription factor

TraesCS4A03G0819500

( TraesCS4A02G330100 )

–7.01

–3.09

613,948,551

613,949,732

Leucine-rich repeat (LRR)-serine/threonine protein kinase

TraesCS4A03G0912300LC

(TraesCS4A02G540400LC)

–5.06

–2.51

641,848,507

641,849,641

Uncharacterised

TraesCS4A03G1099000

(TraesCS4A02G440700)

2.11

1.54

714,457,633

714,463,236

Nitrate excretion transporter (NAXT)

TraesCS4A03G1171000

(TraesCS4A02G466000)

3.78

2.16

737,488,947

737,493,663

Disease resistance protein RGAs/NB-LRR-receptor kinase

TraesCS4A03G1181300

(TraesCS4A02G469600)

8.53

2.74

739,710,098

739,711,681

Aspartyl protease

TraesCS4A03G0741300

(TraesCS4A02G290900)

1.27

1.23

593,189,940

593,190,850

Glycine-rich protein; responss under osmotic stress

TraesCS4A03G0558000

(TraesCS4A02G210100)

1.07

1.07

503,197,597

503,199,959

Flaggerin, di-valent metal binding protein

TraesCS4A03G0542300

(TraesCS4A02G203000)

1.46

1.4

492,653,225

492,654,122

Dirigent protein 1-like; related to stress resistance

TraesCS4A03G0474900

(TraesCS4A02G173800)

–1.11

–2.61

440,895,030

440,896,312

Xylanase inhibitor protein 1

TraesCS4A03G0276500

(TraesCS4A02G130300)

–1.28

–1.43

170,881,259

170,897,924

Phospholipid-transporting ATPase 1

TraesCS4A03G0141900

(TraesCS4A02G070600)

–1.52

–2.62

67,978,564

67,980,472

UDP-glycosyltransferase

  1. Note: Genes in bold are in the Qsns.uwa.4 A.2 marker interval between Xbarc170 and Xgwm350, with a physical position of 607,888,155–739,865,914 bp. The screening marker Xbarc343 (physical position 694,903,704 bp) is within the QTL interval. Upregulated genes (positive value) are those expressed more in resistant (R) isolines, and downregulated genes (negative value) are those expressed more in susceptible (S) isolines. Log2FC is the Log2fold change of the DEGs. Genes in brackets () are from RefSeq1.1.