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Table 1 Gene expression obtained from RNA sequencing between R-isolines and S-isolines of two pairs of near-isogenic lines (NILs) across the whole genome, on chromosome 4 A and within Qsns.uwa.4 A.2

From: Transcriptomic profiling of near-isogenic lines reveals candidate genes for a significant locus conferring metribuzin resistance in wheat

Treatment

Expression pattern

NIL pairs

NIL_3

 

NIL_17

 

Common

Genome

Chromosome 4A

 

Genome

Chromosome 4A

 

Genome

DEGs on Chromosome 4A

DEGs within QTL*

Control

Upregulated

5164 (3.7%)

282 (4.2%)

 

1276 (0.9%)

51 (0.7%)

 

113 (0.1%)

2 (0.03%)

0

Downregulated

4698 (3.3%)

244 (3.6%)

 

1184 (0.8%)

69 (1.0%)

 

86 (0.1%)

5 (0.1%)

3

Total

9862 (7.0%)

526 (7.9%)

 

2460 (1.7%)

120 (1.8%)

 

199 (0.1%)

7 (0.1%)

3

Treatment

Upregulated

1852 (1.3%)

138 (2.0%)

 

2371 (1.7%)

110 (1.6%)

 

59 (0.04%)

6 (0.1%)

3

Downregulated

3456 (2.4%)

203 (3.0%)

 

3931 (2.8%)

171 (2.5%)

 

238 (0.2%)

6 (0.1%)

3

Total

5308 (3.7%)

341 (5.1%)

 

6302 (4.5%)

281 (4.1%)

 

297 (0.2%)

12 (0.2%)

6

  1. * QTL Qsns.uwa.4 A.2 is within the interval markers Xbarc170 and Xgwm350. ~138,790 genes detected according to the reference genome. The number of genes is calculated by dividing ~ 138,790/21 = ~ 6609 to calculate the percentage of upregulated and downregulated genes on chromosome 4 A. The values in brackets are the proportion of genes in the whole genome or on chromosome 4 A, calculated from the number of DEGs divided by the total number of genes detected in the transcriptomic analysis in the whole genome or on chromosome 4 A