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Fig. 5 | BMC Plant Biology

Fig. 5

From: A mutant cotton fatty acid desaturase 2-1d allele causes protein mistargeting and altered seed oil composition

Fig. 5

Light microscopy analysis of cellular fluorescence produced by baker’s yeast cells expressing plant FAD2/NeonGreen protein fusions. Construct carrying NeonGreen fluorescent protein alone is included as a control (A). Other constructs contain NeonGreen appended near the respective C-termini of AtFAD2 (AtFAD2-cNeonGreen, D), mutant fad2-1d (fad2-1d-cNeonGreen, G), and repaired FAD2-1D (FAD2-1D-cNeonGreen, J). Bright field images and merged images bright field and fluorescing cells are shown in panels B, E, H, and K, and C, F, I, and L, for NeonGreen alone, AtFAD2-cNeonGreen, fad2-1d-cNeonGreen, and FAD2-1D-cNeonGreen, respectively. The scale bar included in all panels = 10 μm

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