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Fig. 2 | BMC Plant Biology

Fig. 2

From: Identification and characterization of CircRNA-associated CeRNA networks in moso bamboo under nitrogen stress

Fig. 2

Validation of circRNAs. a The circRNA (PeSca_13:101,468,494|101,470,730) exemplified the validation strategy. According to the sequence of PeSca_13:101,468,494|101,470,730, two sets of arrows indicate two sets of amplification primers, which were designed to confirm head-to-tail back-splicing by PCR and Sanger sequencing. b-d Validation of PeSca_13:101,468,494|101,470,730, PeSca_15:80,000,303|80,002,729, and PeSca_21:100,130,199|100,130,642 by PCR and Sanger sequencing. Convergent primers amplified the circRNAs in both cDNA and gDNA. Divergent primers successfully amplified the circRNAs in cDNA but not those in gDNA. e–g Relative expression of three selected genes validated by qPCR. Error bars indicated standard deviation (SD) of three technical repeats. RNase R + and RNase R- represented total RNA treated with and without RNase R, respectively

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