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Fig. 8 | BMC Plant Biology

Fig. 8

From: Novel R2R3 MYB transcription factors regulate anthocyanin synthesis in Aubergine tomato plants

Fig. 8

Virus Induced Gene Silencing (VIGS) of AN2like in Abg fruits. a Representative picture of a homozygous Abg (Abg/Abg) fruit that underwent VIGS with the empty TRV2 vector (left) and of a Abg/Abg fruit that underwent VIGS with the TRV2 vector carrying the silencing construct for the gene AN2likeAbg (right). Both fruits were collected at the mature green stage. b Anthocyanin quantification in the fruit peel of unsilenced (VIGS + empty TRV2 vector) and silenced (VIGS + TRV2 vector silencing the gene AN2likeAbg) Abg/Abg fruits. Anthocyanins are expressed in mg petunidin-3-(p-coumaroyl rutinoside)-5-glucoside g− 1 fresh weight (FW). Data are means of six biological replicates. Unpaired t-test was carried out and **** asterisks indicate significant difference (P ≤ 0.0001). c qPCR analysis of the regulatory R2R3 MYB gene AN2like and of the structural late biosynthetic genes DFR and ANS in the peel of Abg/Abg unsilenced or silenced fruits. Data are means of six biological replicates. Unpaired t-test was carried out and *** asterisks indicate significant difference (P ≤ 0.001) and **** asterisks indicate significant difference (P ≤ 0.0001). Photographs are from the authors

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