Fig. 6
From: Novel R2R3 MYB transcription factors regulate anthocyanin synthesis in Aubergine tomato plants
Functional in vivo analyses of AN2likeAbg and MYB113Abg. a Anthocyanin accumulation in leaves of Nicotiana benthamiana agroinfiltrated with plasmids containing the AN2like long and short transcript sequences of Abg or Aft co-expressed with the effector plasmid containing the bHLH factor AN1, also used alone as negative control. White dotted circles indicate the agroinfiltrated areas. b Quantification of the anthocyanins produced in the different areas of the leaves agroinfiltrated as described in a. c Anthocyanin accumulation in N. benthamiana leaves agroinfiltrated with plasmids containing the MYB113 genomic sequences of Abg or wild type (WT) plants co-expressed with the effector plasmid containing the bHLH factor AN1, also used alone as negative control. As positive control, the AN2likeAbg long transcript was co-expressed with AN1. White dotted circles indicate the agroinfiltrated areas. d Quantification of the anthocyanins produced in the different areas of the leaves agroinfiltrated as described in c. In b and d anthocyanins are expressed in mg petunidin-3-(p-coumaroyl rutinoside)-5-glucoside g− 1 fresh weight (FW). Data are means of ten biological replicates. One-way ANOVA with Tukey’s HSD post hoc test was performed. Different letters indicate significant differences at P ≤ 0.05. Photographs are from the authors