Fig. 3

Fold relative expression of candidate genes in rubber tree leaves analyzed by RT-qPCR at 10, 30, and 50 days after R. microporus inoculation compared to mock-inoculation at 30 DAI (control). The expression levels of target genes were normalized with the UBC2a reference gene [46]. The data obtained from 3 biological and 9 technical replicates (N = 27) were analyzed for the fold relative expression, following the modified method described by Taylor et al. [90]. Error bars indicate one standard error. Statistical analysis was performed with an analysis of variance (ANOVA) and Tukey’s post hoc significance test at p < 0.05, groups with different letters are significantly different