Table 2 Polymorphic and linkage analysis of the markers linked to the powdery mildew resistance genes located on chromosome arm 2BL using the mapping population derived from the cross of ShiCG15–009 × Yannong 21
Marker | Resistance genes | Polymorphism | Linkage to PmCG15–009 | Forward primer (5′–3′) | Reverse primer (5′–3′) | Cultivars | References | |
|---|---|---|---|---|---|---|---|---|
Parents | F2:3 bulks | |||||||
CIT02g-1 | Pm6 | – | – | – | TGTCACCTACCCATTCAGCT | TTCTCCAATGCTTCGAGTGC | Coker747 | [15] |
CIT02g-2 | Pm6 | + | + | + | GAGAGCATTCGTCGGTTTCC | ATTCGACCGCCTCAAATCCA | ||
CIT02g-3 | Pm6 | – | – | – | GACCGTGCCTTCCATTGTTG | TGTTCACACAAGCAGCAAGT | ||
CIT02g-4 | Pm6 | – | – | – | TGACCCTAAAACAGTCTCAAAGA | TGTTGTAAATGAGAAGTGCACCT | ||
CIT02g-5 | Pm6 | – | – | – | GGTCACCTTCTTCATAGCGC | GGTCACCTTCTTCATAGCGC | ||
CIT02g-6 | Pm6 | – | – | – | CGGCATCGTCCAGGAAATG | TGCTTTGGTTCGAGTTGGTG | ||
CIT02g-7 | Pm6 | – | – | – | CCTCTCTTCCTGTCCCTTATGG | ACTACCGATGAGAGTTCCAGA | ||
CIT02g-8 | Pm6 | – | – | – | AAGAAAGCGCGCACCATG | GCAGTCCACGAACCGCTC | ||
CIT02g-9 | Pm6 | – | – | – | AAATCGAAGCCTTGCACCAA | GGACAAAGTGCGCGAAGT | ||
CIT02g-10 | Pm6 | – | – | – | TGGGACTGGTTAGCACTTGA | CGATGAGGAATAAGTGGGCA | ||
CIT02g-11 | Pm6 | – | – | – | CAAAGCTTGCAAGATGGGTG | TTCCAGCCCCTCTAGTGATC | ||
CIT02g-12 | Pm6 | – | – | – | TGGAACGTCTAGACCACAGG | TGGAACGTCTAGACCACAGG | ||
CIT02g-13 | Pm6 | – | – | – | AGAGAAGTGGAGGTGATGGC | CACGGAGGCTGGGTTCAC | ||
CIT02g-14 | Pm6 | – | – | – | TCTTCCTCTCTTCCTGTCCC | ACTACCGATGAGAGTTCCAGA | ||
CIT02g-15 | Pm6 | – | – | – | GAGAGCATTCGTCGGTTTCC | GCTTCCTGGATCATCTGAGC | ||
CIT02g-16 | Pm6 | – | – | – | GCATCAATAAATCCCTTTCTGCA | TTTCCTCCAGTTCATCGCCC | ||
CIT02g-17 | Pm6 | + | + | + | CTGGATGAACTTCCCCAAAA | TCAATCTTGAACATCTCCCTCA | ||
CIT02g-18 | Pm6 | + | + | + | GGCCTTAGTGGTGATGCAGT | GCGGCTTGTCGGTGTATAG | ||
CIT02g-19 | Pm6 | – | – | – | TCGTTCACACTCAACTCCCA | AGCGAGATCCCATGACTGAC | ||
CIT02g-20 | Pm6 | + | + | + | CGTGCCTTCCATTGTTGTAT | TGTTCACACAAGCAGCAAGTT | ||
CIT02g-21 | Pm6 | – | – | – | TTTGGGCCTGCGACGATC | ACGGTGTTATTCCTAGCATGC | ||
CIT02g-22 | Pm6 | – | – | – | CTCTACGAGCTGTCTTCGCT | TCCCTTGGTAGTACTTGGACA | ||
CISSR02g-1 | Pm6 | – | – | – | TGTCATTTACTCGTGTGCTTCA | CCTTACGCTTTCCTCATAAACC | ||
CISSR02g-2 | Pm6 | – | – | – | GACTACAACTACCTTCCCGTGG | AGGATGAAAACCTCGACACACT | ||
CISSR02g-3 | Pm6 | + | + | + | CTAAACCATAAGCAATCCCCTG | GTCTACAACTACCTTCCCGTGG | ||
CISSR02g-4 | Pm6 | – | – | – | TTCGTAGGTTTTGTGCATGTTC | AGTTAGGGTAGGAAGAGGTGGG | ||
CISSR02g-5 | Pm6 | – | – | – | ACTTCCAGCAAATGTTGTAGCC | GTCGAGAGTTGAGGGTCGTC | ||
CISSR02g-6 | Pm6 | + | + | + | TAAGCAACATCTCATCCCCTTT | GAATACGCCTCCACTCATACCT | ||
CINAU117 | Pm6 | – | – | – | GACCCAAGAGGCGTTGATTA | CATGTGTGCCAAATTCAAGC | [16] | |
CINAU118 | Pm6 | – | – | – | GCTGTGACTGCTGGATTCAA | ACCGGGACTGTGTAGACTGG | ||
CINAU119 | Pm6 | – | – | – | CTTCGTTGCTCGAAAGGTTC | CGGGTGAAACATCTTCTGGT | ||
CINAU120 | Pm6 | – | – | – | GCCATGGCTAAGGAAGAAGA | ACCTTGGCGAGCTTCTTGAC | ||
CINAU121 | Pm6 | – | – | – | CCTAGACTGGCCAAGACGAT | ATGGTTTGATTCACCAGCAA | ||
CINAU122 | Pm6 | – | – | – | CACCTACCTCGTCAACGG | GAGTGCTCCACTGTAAAGCC | ||
CINAU123 | Pm6 | – | – | – | TTGTACGCCATCGACACATT | CCGAACAGAGTTTTGCCTTC | ||
CINAU124 | Pm6 | – | – | – | GAGTGCTCCACTGTAAAGCC | CACCTTTGTAGACAGTCCCG | ||
CINAU125 | Pm6 | – | – | – | CCTCTTCCTGACCATCTTCC | TGACAGTCACTCCAATCACG | ||
CINAU126 | Pm6 | – | – | – | TCATTTGGTTGCATAGTTGC | AATTTAGCAGTATTCTTAGCTTCCC | ||
CINAU127 | Pm6 | – | – | – | AATTTAGCAGTATTCTTAGCTTCCC | ATGGGCCGTACAAGAAAGTG | ||
CINAU128 | Pm6 | – | – | – | TCGAACATGGCTGTGATGAT | GGCTCAGCTTTACCAAGAGC | ||
CINAU129 | Pm6 | – | – | – | ATCTTGCAGCTTTTGCGTTT | GCTCCCTGACACTCTTGAGG | ||
CINAU130 | Pm6 | + | + | + | GGCGAGAAAATGTTGTCCAT | AGAAGAGCTGGAGCACCTTG | ||
CINAU131 | Pm6 | – | – | – | CAACTGCTGGCTCTTCTTCC | GGAACAGCAGCGTCTTCTTC | ||
CINAU132 | Pm6 | – | – | – | GTGGCTACACCCAAACGG | CAGATCAACGGGAGACATCAC | ||
CINAU133 | Pm6 | – | – | – | AAGAACCATATCTGGGCTGTC | TACAACAAGATGCCGCAGGCTAACA | ||
CINAU134 | Pm6 | – | – | – | ATCAACAAGATCTTCGACGG | CTTTGTCTGAACATTGCTGC | ||
CINAU135 | Pm6 | – | – | – | TTGGTGACGCAGTAATGGAA | TGTGACAGAGCTAGGGCAAG | ||
CINAU136 | Pm6 | – | – | – | CTGACTGCGCCTTATGTTGA | CCGTGGCTTGATGGAGTCATA | ||
CINAU137 | Pm6 | – | – | – | GGACAATGAGAAAGCAAAGG | CTTTGCAAGAGCATCAGAGG | ||
CINAU138 | Pm6 | – | – | – | TTCCCGAAGGACTACCATTG | TCCAGTCACCTCTGGAGCTT | ||
CINAU139 | Pm6 | – | – | – | CAAAGGAGCCTTTCGATGAG | GGATTCGGGTAGCTTGCATA | ||
CINAU140 | Pm6 | – | – | – | CACGGTGGAAGTCACTAACC | CAGTTTCCAAGGCATAGGG | ||
CINAU141 | Pm6 | + | + | + | CACACATGGCAAGTTACAGG | ATCAGACTTGCTTGCTCACC | ||
CINAU142 | Pm6 | + | + | + | CGACTACGTGACGCTCAAGA | ACTTGTCGTCGAGGAGGATG | ||
CINAU143 | Pm6 | + | + | + | GTTGGTGGTTGAAAAGATGG | AGTATGCACCTTCGATTTGC | ||
CINAU144 | Pm6 | – | – | – | GCTCCTCAGCAAATGCCTAC | GATGAAGTGGTGAGCAAGCA | ||
NAU/STSBCD135–2 | Pm6 | – | – | – | GCTCCGAAGCAAGAGAAGAA | TCTGCTGGTCCTCTGATGTG | [17] | |
Xwmc317 | Pm33 | – | – | – | TGCTAGCAATGCTCCGGGTAAC | TCACGAAACCTTTTCCTCCTCC | Am9/3 | [18] |
Xgwm526 | Pm33 | – | – | – | CAATAGTTCTGTGAGAGCTGCG | CCAACCCAAATACACATTCTCA | ||
BQ246670 | Pm51 | – | – | – | ACATGAGTGAGTTGTGAGTC | AGAAGGCACACTGCTGGAAC | CH7086 | [19] |
BE444894 | Pm51 | – | – | – | CAATGGGGGTCTTATGGATG | GATGTTGCAGACGGGGTAGT | ||
BE405017 | Pm51 | – | – | – | CTTACTGGTGGACATGGGCT | CGCAGGGCTATCTTGTTCTC | ||
Xbarc159 | Pm51 | – | – | – | CGCAATTTATTATCGGTTTTAGGAA | CGCCCGATAGTTTTTCTAATTTCTGA | ||
Xwmc332 | Pm51 | – | – | – | CATTTACAAAGCGCATGAAGCC | GAAAACTTTGGGAACAAGAGCA | ||
Cos66 | Pm51 | – | – | – | CACGGTGGAAGTCACTAACC | CAGTTTCCAAGGCATAGGG | ||
Xicsl34 | Pm52 | – | – | – | GTCCAATCGATCAACTTCAG | GACTAGCTCGCTCTGGATTA | Liangxing 99 | [20] |
Xicsl62 | Pm52 | – | – | – | AGCAAAGCAATTAGGAGAGTT | CTGCGACTGTTTTCTTTTAAC | ||
Xicsl90 | Pm52 | – | – | – | AGACTGGGTGCTAGTTGTGT | TGACTTGTCACTGGTTTTCTC | ||
Xicsl163 | Pm52 | – | – | – | GAGAGTACAAAAGGCAGAGG | ACATAGGGAAATCGAATAAGG | ||
Xicsl224 | Pm52 | – | – | – | TGCTGTGCTACTTTTGCTACT | TCTCCCAATCTATCAACGTAA | ||
Xicsl234 | Pm52 | – | – | – | TCTCAGTTTTCACCTCCACTA | CCTTGCTAGAAAAAGGAGAAT | ||
Xicsl275 | Pm52 | – | – | – | CCGTCCGTATATTCAATTACTC | GCGTTTGCAAGTACAGACTAC | ||
Xicsl306 | Pm52 | – | – | – | GCGTTTGCAAGTACAGACTAC | GTAGTAAAATGGCAGCAGAGA | ||
Xicscl437 | Pm52 | – | – | – | CTGTTAGCAAGAACCATTAGG | GGAATAGCTGGAAGTCTTCTG | ||
Xicscl445 | Pm52 | – | – | – | GGAATAGCTGGAAGTCTTCTG | TAAACAACTCCATGGTTCAGT | ||
Xicscl726 | Pm52 | – | – | – | GCTGCTGAGTAGCTGTATGAG | CTATCATGGAACTTGCAAAAC | ||
Xicscl795 | Pm52 | – | – | – | GTCAACCTCATCTTCTCCTG | GTCAACCTCATCTTCTCCTG | ||
Xicssl173 | Pm52 | – | – | – | GGAAACTCAATTCATCACAAG | GGCTGAGGGTATGTACAAGTAG | ||
Xicssl174 | Pm52 | – | – | – | AACAAGCTTAACGTGTACCAA | AAAGCTTGCATGCTATAATGT | ||
Xicssl326 | Pm52 | – | – | – | AAGATGCACTTACCCAAAAAC | TGCTACATATAACTGCTGCTG | ||
Xwmc175 | Pm52 | – | – | – | GCTCAGTCAAACCGCTACTTCT | CACTACTCCAATCTATCGCCGT | [21] | |
Xwmc441 | Pm52 | – | – | – | TCCAGTAGAGCACCTTTCATT | ATCACGAAGATAAACAAACGG | ||
Xgwm120 | Pm52 | – | – | – | GATCCACCTTCCTCTCTCTC | GATTATACTGGTGCCGAAAC | ||
Xbcd135–2 | Pm63 | – | – | – | GCTCCGAAGCAAGAGAAGAA | TCTGCTGGTCCTCTGATGTG | PI 628024 | [22] |
Xstars419 | Pm63 | – | – | – | GCCCTTGTCAGTTTCAGTCC | GTCGATCGCTCCACCTCTAC | ||
Xgwm120 | Pm63 | – | – | – | GATCCACCTTCCTCTCTCTC | GATTATACTGGTGCCGAAAC | ||
Xwmc175 | Pm63 | – | – | – | GCTCAGTCAAACCGCTACTTCT | CACTACTCCAATCTATCGCCGT | ||
Xwmc441 | Pm63 | – | – | – | TCCAGTAGAGCACCTTTCATT | ATCACGAAGATAAACAAACGG | ||
Xwmc332 | Pm63 | – | – | – | CATTTACAAAGCGCATGAAGCC | GAAAACTTTGGGAACAAGAGCA | ||
WGGBH1364 | Pm64 | – | – | – | CCAAGAAATGGAGTGTTTGA | CAATTATTGGGATCAACACC | WE35 | [23] |
WGGBH218 | Pm64 | – | – | – | CCTTCCTCCGGTAACTCATA | CGAGCTAGCAATCAGAGAAG | ||
WGGBH1099 | Pm64 | – | – | – | CGAGCTAGCAATCAGAGAAG | AGGCGGTCTACTGGATTATATGT | ||
WGGBH913 | Pm64 | – | – | – | ACTGAAACGACAGCTTTTAGG | GGTGAGCTAGTTTGCTCTGTT | ||
WGGBH252 | Pm64 | – | – | – | GGTGAGCTAGTTTGCTCTGTT | GGATTGGACTATTAGTCAACG | ||
WGGBH1212 | Pm64 | – | – | – | AACCTCAGTAACCATTGCCAAG | CTCACGCCTTCAACTCATCAG | ||
WGGBH612–5 | Pm64 | – | – | – | TCTTGCCCTTGTCAGTTTCAG | TACGTGCGAGTAAGAGTAGGAG | ||
WGGBH134 | Pm64 | – | – | – | AGCTTGAATGAGGATGAAGAGT | CTTCTCTTTCTCCTTCTCCGAA | ||
WGGBH686 | Pm64 | – | – | – | CAGGGTACTGTATCAGTGTGG | AAGTGATAACACAGCTTGTCG | ||
WGGBH1260 | Pm64 | – | – | – | GACTTGCTCCTGCCTGCTA | TTCTTGGAATGTTCTGCGTGAT | ||
YTU130–129 | PmCG15–009 | + | + | + | ATCGGGAAGGCATGGTCAAG | CGAGAGGATAAGGCCGAACC | ShiCG15–0 | |
YTU130–130 | PmCG15–009 | + | + | + | GTGTACGGCAAGGTGACAGA | ATGGCAAGACTGTGGGTACG | 09 | |
YTU130–97 | PmCG15–009 | + | + | + | CTAGGGCTGGACCAGTTTGG | AGTTGTGGAAATCGGCGGAT | ||
YTU130–101 | PmCG15–009 | + | + | + | GGGAGAGCCGTCAAAGAACA | CTTCTCATTTTCTCCGCGCG | ||
YTU130–46 | PmCG15–009 | + | + | + | CTTCCTCCATTGACCACGCT | GCGAGAGATTCATCCAGCGA | ||
YTU130–105 | PmCG15–009 | + | + | + | TCGAGGCGCTTCTTCACTTT | TTGCAATGGTGTTGCTCTGC | ||
YTU130–109 | PmCG15–009 | + | + | + | CCGATTACCTGCAGCTCGAT | TCCAGCTTGGACTTGTCGAC | ||
YTU130–54 | PmCG15–009 | + | + | + | AGGGCAAAAGATGGAGGTCG | TCGTTCAAGGGCATCAGCAT | ||
YTU130–115 | PmCG15–009 | + | + | + | AGGAGCTTCATGGCCTTCAC | TCACTGTGAGCGACTGACAC | ||
YTU130–69 | PmCG15–009 | + | + | + | CGAGCGTGATGTAGACCTCC | GTTTTTCCAGGCCAGCAAGG | ||