Fig. 4From: Promoter activity and transcriptome analyses decipher functions of CgbHLH001 gene (Chenopodium glaucum L.) in response to abiotic stressAnalysis of CgbHLH001 expression in C. glaucum and identification of transgenic Arabidopsis overexpressing CgbHLH001. A-B Detection of CgbHLH001 expression in C. glaucum under different abiotic stress at mRNA and protein levels. C-F Identification of CgbHLH001 in transgenic Arabidopsis by PCR, RT-PCR, qRT-PCR and Western blot. In A, different lowercase letters above the columns indicate significant differences (P < 0.05) between different treatments; in C, + : positive control; -: negative control; M: DL2000 DNA marker; in D, -: negative control; Atactin served as the internal reference; in E, different lowercase letters above the columns indicate significant differences (P < 0.05) between different Arabidopsis lines; in F, Tubulin served as the internal reference. Original images of gels and blots were present in Supporting Fig. 1 in the Additional file 14Back to article page