Fig. 4

Analysis of CgbHLH001 expression in C. glaucum and identification of transgenic Arabidopsis overexpressing CgbHLH001. A-B Detection of CgbHLH001 expression in C. glaucum under different abiotic stress at mRNA and protein levels. C-F Identification of CgbHLH001 in transgenic Arabidopsis by PCR, RT-PCR, qRT-PCR and Western blot. In A, different lowercase letters above the columns indicate significant differences (P < 0.05) between different treatments; in C, + : positive control; -: negative control; M: DL2000 DNA marker; in D, -: negative control; Atactin served as the internal reference; in E, different lowercase letters above the columns indicate significant differences (P < 0.05) between different Arabidopsis lines; in F, Tubulin served as the internal reference. Original images of gels and blots were present in Supporting Fig. 1 in the Additional file 14