Fig. 3
Analysis of CgbHLH001 promoter activity under abiotic stress. A Relative expression of GUS gene driven by PbHLHFL, PbHLH364, and PbHLH1148. CaMV35S promoter (P35S) was used as the positive control; Col was used as the negative control. PbHLHFL: full-length CgbHLH001 promoter driving GUS gene; PbHLH364: 5’ UTR sequence of CgbHLH001 promoter driving GUS gene; PbHLH1148: 5’ UTR deletion of full-length CgbHLH001 promoter driving GUS gene. B GUS staining of transgenic Arabidopsis seedlings. C Detection of GUS enzyme activity. The GUS activity was expressed as nmol 4-methylumbelliferone per minute per mg protein. Bars represent means ± SD of three biological replicates. Different lowercase letters indicate significant differences (P < 0.05) existing among different treatments in A and C