Fig. 8

Interaction analysis of PtSMXL clade II members with PtD14a/b by yeast two hybrid experiments. D14a and D14b were fused to GAL4-BD. SMXL7a, SMXL7b, SMXL8a and SMXL8b were fused to GAL4-AD. Yeast cells were co-transformed with D14a/D14b and SMXL7a, SMXL7b, SMXL8a, SMXL8b or empty AD vector. Yeast cultures were spotted onto selective growth medium -LT or -LTH (−H, −His; −L, −Leu; −T, −Trp). These media were supplemented with 20 μM GR24, or 0.02% acetone control. Images show growth after 3 d at 30 °C