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Table 1 Sequence of the dKASP markers for the major genes controlling plant height, grain hardness, and high-molecular-weight glutenin subunits

From: Cost-effective duplex Kompetitive Allele Specific PCR markers for homologous genes facilitating wheat breeding

Marker

Trait

Gene

Sequence

Genotype

Rht-BD

PH

Rht-B1/Rht-D1

F1: CCCATGGCCATCTCCAGCTA (Rht-B1b)

F2: ATGGCCATCTCGAGCTGCTA (Rht-D1b)

C: GCTCGGGTACAAGGTGCG

A: Rht-B1b/Rht-D1a

B: Rht-B1a/Rht-D1b

C: Rht-B1b/Rht-D1b

D: Rht-B1a/Rht-D1a

Pin-ab

GH

Pina-D1/Pinb-D1

F1: TGACAACCTCCCTTCCACCA (Pina-D1a)

F2: CTCATGCTCACAGCCGCC (Pinb-D1a)

C: ATGAAGGATTTYCCRGTCACCTG

A: Pina-D1a/Pinb-D1b

B: Pina-D1b/Pinb-D1a

C: Pina-D1a/Pinb-D1a

D: Pina-D1b/Pinb-D1b

Glu-AD

HMW-GS

Glu-A1/Glu-D1

F1: ATAGTATGAAACCTGCTGCGGAC (Dx5)

F2: CTTGGCCTGGATAGTATGAAACCC (Null)

C: GGGAATACCTGCACTACTAARAAGG

A: Ax1 or Ax2*/Dx5

B: Null/Dx2

C: Null/Dx5

D: Ax1 or Ax2*/Dx2