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Fig. 7 | BMC Plant Biology

Fig. 7

From: Genome-wide identification and expression analysis of wall-associated kinase (WAK) and WAK-like kinase gene family in response to tomato yellow leaf curl virus infection in Nicotiana benthamiana

Fig. 7

Silencing of the NbWAK/WAKLs in Nicotiana benthamiana makes it susceptible to infection with tomato yellow leaf curl virus (TYLCV). a Silencing efficiency was assessed by quantitative PCR (qPCR) at ten days post-inoculation (dpi). The values represent relative mRNA levels against those of the control groups (N. benthamiana seedlings agroinfiltrated with TRV:GFP), values of which are set to 100%. b Disease symptoms caused by TYLCV in the NbWAK/WAKLs silenced N. benthamiana seedlings at 21 dpi. TRV:GFP-agroinfiltrated N. benthamiana seedlings infected with TYLCV were used as the control. c Relative accumulation of TYLCV genomic DNA in the NbWAK/WAKLs silenced N. benthamiana seedlings. Viral accumulation was measured by qPCR at 21 dpi, as shown in Fig. 7a. The values represent relative viral DNA accumulation levels against those of the control groups (TRV:GFP-agroinfiltrated N. benthamiana seedlings infected with TYLCV), values of which are set to 1.0 units. For a and c, the data are given as means ± standard deviation of three biological replicates. Significant differences in expression are marked with asterisks: * P < 0.05 or ** P < 0.01; Student’s t-test

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BMC Plant Biology

ISSN: 1471-2229

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