Fig. 6

Pipelines used to predict effectors of A. solani infection. A. Venn diagram showing the predicted number of secreted proteins. The circles with different colours indicate the results predicted by different analysis software; signalp indicates a protein containing an N-terminal signal peptide as predicted by signalp-4.1; tmhmm indicates a tmhmm-2.0c prediction of proteins containing transmembrane domain(s); phobius indicates phobius 101 predicted protein carrying an N-terminal signal peptide but no transmembrane domain; and protComp indicates Protcomp v3 prediction of proteins with a subcellular location or that was secreted from the outer cell membrane. The number of proteins predicted by ProtComp that that were also predicted signalp or phobius but not by tmhmm, was predicted to be a secretory protein. The red numbers indicated predicted secreted proteins. B. Heatmap of 85 SSP genes after an expression pattern cluster analysis. The colour in the figure indicates the expression level of a gene after standardization in each sample: red indicates gene expressed at a high level, blue indicates a gene expressed at a low level, and the number under the upper left colour bar indicates the specific expression level