Fig. 7From: Brassinosteroids and gibberellic acid actively regulate the zinc detoxification mechanism of Medicago sativa L. seedlingsEffects of Zn, BR and GA on the expressions of Zn chelation and transport genes. Total RNA was isolated from M. sativa roots and shoots. The MsEF1α gene from M. sativa was used as the housekeeping gene for normalization. The expressions of MsHMA4 (A), MsYSL1 (B) and MsNAS1 (C) genes involved in Zn chelation and long-distance transport were determined in the roots and shoots of M. sativa after 7 weeks of treatment. ANOVA (including Brown-Forsythe test and Bartlett test) and Tukey’s multiple comparisons test were performed on the data of 3 biological replicates (n = 3). Vertical bars represent mean ± SD. Averages with the different letter are significantly different at p < 0.05Back to article page