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Fig. 6 | BMC Plant Biology

Fig. 6

From: Brassinosteroids and gibberellic acid actively regulate the zinc detoxification mechanism of Medicago sativa L. seedlings

Fig. 6

Effects of Zn stress, BR and GA on the expressions of genes related to Zn-efflux. Total RNA was isolated from M. sativa roots and shoots. The MsEF1α gene from M. sativa was used as the housekeeping gene for normalization. The expressions of MsMTP1 (A) and MsZIF1 (B) genes involved in Zn efflux and vacuolar storage were determined in roots and shoots of M. sativa after 7 weeks of treatment. ANOVA (including Brown-Forsythe test and Bartlett test) and Tukey’s multiple comparisons test were performed on the data of 3 biological replicates (n = 3). Vertical bars represent mean ± SD. Averages with the different letter are significantly different at p < 0.05

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