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Fig. 5 | BMC Plant Biology

Fig. 5

From: Auxin efflux carrier ZmPIN1a modulates auxin reallocation involved in nitrate-mediated root formation

Fig. 5

Effect of LN supply on the relative expression of ZmPIN1a in roots. A-B The relative expression of ZmPIN1a in PR tip (5 mm of the root tip) (A) and LRP initiation zone (5–25 mm behind the root tip) (B) at different time points after LN and NN treatments. Values with error bars represent mean ± SD (n = 3). The gene expression was calibrated to the expression of ZmUBC (ubiquitin C). C-D In-situ hybridization of ZmPIN1a in root at 12 h after LN and NN treatment. Longitudinal and transverse sections of maize PR tip (0–2 mm of the root tip) (C) and LRP initiation zone (7–10 mm behind the root tip) (D) probed with the ZmPIN1a antisense or sense probes. Bar = 100 µm. The asterisks indicated significant differences between different treatments according to a Student's t test. **, P ≤ 0.01

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