Development of unigene-derived SSR markers from RNA-seq data of Uraria lagopodioides (Fabaceae) and their application in the genus Uraria Desv. (Fabaceae)

Table 2 Characteristics of 19 polymorphic unigene-derived SSRs

SSR locus	Forward primer (5'-3')	Reverse primer (5'-3')	Repeat motif	Ta (℃)	Observed product size (bp)	Na
P6	GACACGCTCATGTCGCATTC	GAGCATTTGAAGTCGGTGCG	(TG)8	55	194–227	8
P8	ACGGTCGATGAGTTTGTCGT	ACTGCTGTGCTGCTGTAGAA	(AG)8	55	231–297	2
P25	TGATGTCCTCCACATGTGCC	GCCACTCACCTAGAAGAGCC	(GTGA)7	55	268–281	5
P30	AGATTGCAGCAAGGGTCTCA	GGCACCTCCAATTCCTAGTCA	(TTCT)5	54	177–195	3
P32	CCAGCAGGTACATGTCTGGG	AGGTATTGTAGTCAAAGAACCAGT	(GT)9	53	168–177	3
P38	AGAGTTGCGACGCATAACCA	CCGTAAAGTCCGCGTGAAAC	(TA)8	55	225–265	3
P39	ACGCATATCGGGTCAGTAAACA	GTATTACAAATATTACATGCACACTGC	(GT)6(T)11	53	205–247	5
P40	TGGTTGACCGCAGCATAGTT	GAAGGTGCTTGTCCATGGGA	(TGG)6	55	458–498	7
P43	TGCTGCACCCTTCACATTCA	GGGTCGAGTTTGAGGAGTCG	(AC)8	55	224–255	4
P46	GGAGCAATGTCACCAAGGGA	CCATCTTAGAGCTGGCCACA	(GATG)5	55	355–370	1
P55	GCAGGTTTACGTCCAATGCT	ACAGTGTGCCCTCACGAAAT	(TATC)5	54	217–247	3
P59	ACCCTTCACAAACTCCCTCTC	AGGCTGAAAGAATGGCCTCC	(TTG)6	54	260–274	2
P71	CCACACCACAGCAGAACAGA	GTTCGGGCAAGGGAAGAGAA	(AC)6*(CT)9	55	154–225	10
P127	TCAACCACCGTAGCTGAACC	CGGATCTGAGAGCGGAAGAC	(GGA)6	55	231–277	5
P131	TGACAAGCCTTCCCGAACTC	CTCTAGGCACCTGCAAGAGA	(TTTA)5	54	243–251	2
P132	CGTTCGAGACCCAAGTCAGT	AGCATCACAGCCATCAACGA	(ATTA)5	55	237–286	2
P138	CAGCGTCCTCTGTGTTCTGT	AGTTCCGATTTCCGAAGCGA	(AT)9	55	217–285	7
P141	GAGGAGAAGGCGGTGATGAC	TAGAAGCAGCACCACCACTG	(TGG)7	55	275–296	3
P149	ACCGCAACTCATTACCTCCT	AGAATTCAGCACACGGAGCA	(TA)10	54	120–176	5
*GCACCTTACTCACTCTCTGTTACTCTTCACACA

ISSN: 1471-2229