Fig. 2
From: The study on interacting factors and functions of GASA6 in Jatropha curcas L.
Prokaryotic expression and identification of JcGASA6. A Whole bacteria SDS-PAGE analysis of JcGASA6 expression. B SDS-PAGE analysis of precipitation from bacteria. C SDS-PAGE analysis of supernatant from bacteria. M, marker; lane 0, control (empty pColdII); lane 1–3, expression of JcGASA6 in BL21(DE3); lane 4–6, expression of JcGASA6 in ESLa; lane 1and 4, induction with 0.10 mM IPTG for 16 hours; lane 2 and 5, induction with 0.25 mM IPTG for 16 hours; lane 3 and 6, induction with 0.25 mM IPTG for 4 hours; red arrow indicates JcGASA6 protein. D Mass spectrometry identification results of expression product from JcGASA6