Cloning and characterization of nitrate reductase gene in kelp Saccharina japonica (Laminariales, Phaeophyta)

BMC Plant Biology

Table 1 All primers used in this research

Amplification	Primers	Sequences (5′-3′)
cDNA fragment	SjNR-F	CTCCCACTCGGTCGCAAC
cDNA fragment	SjNR-R	GCAAGCTGAACTTAGAAG
RACE	5-GSP^a	GATTACGCCAAGCTTGAAGTTGAAGTGGCTGGTGCTGCTG
	3-GSP^a	GATTACGCCAAGCTTCGCCAACGGCTACAGCAGCACCAG
	Long primer^b	CTAATACGACTCACTATAGGGCAAGCAGTGTATCAACGCAGAGT
	Short primer^b	CTAATACGACTCACTATAGGGC
qPCR	SjNR-qPCR-F	GGACGGGAGTGTGGGTGAAGGA
	SjNR-qPCR-R	CGTTGATGATGTAGGGGGGCTT
	SjUBA80-qPCR-F	AAGAAGACCTACACCAAGCCTA
	SjUBA80-qPCR-R	GTTTGGACGACACGGCACAGCG
CDS	pCold-SUMO-SjNR-L-F^c	ggtaccctcgagggatccATGGCCTCAGGAATCCAATCG
	pCold-SUMO-SjNR-L-R^c	gcagagattactctagaGAAGTTGAAGTGGCTGGTGC
	pCold-SUMO-SjNR-S-F^c	ggtaccctcgagggatccATGGAGATGAGCGTGGAGTAC
	pCold-SUMO-SjNR-S-R^c	gcagagattactctagaAAAGTTGAAGTGGCTGGTGC
	pIC9K-SjNR-F^d	agcttacgtagaattcATGGCCTCAGGAATCCAATCG
	pIC9K-SjNR-R^d	aattaattcgcggccgcTTAGAAGTTGAAGTGGCTGGTGC
	5’AOX1^e	GACTGGTTCCAATTGACAAGC
	3’AOX1^e	GCAAATGGCATTCTGACATCC

^a Capital with underline indicates the homologous sequence with the end of pRACE vector for ligation by recombination
^b Capital italics indicates the primer provided by the SMARTer RACE 5′/3′ Kit
^c Lowercase indicates the homologous sequence with the end of pCold-SUMO vector for ligation by recombination
^d Lowercase italics indicates the homologous sequence with the end of pPIC9K vector for ligation by recombination
^e Capital italics with underline indicates the sequence of universal primer for pPIC9K vector

ISSN: 1471-2229