Fig. 5

IPA1 plants susceptible to drought stress in rice. A DAB staining for H₂O₂ in leaves from oxidative stressed WT and IPA1 mutant plants. B, C, D MDA, POD and CAT content of the WT and IPA1 mutants during drought stress treatment. E Expression levels of ROS-related genes in WT (FH7185) and IPA1-CAS9 in response to drought stress. The relative expression levels of ROS-related genes were determined by qRT-PCR using gene-specific primer sets (Supplementary Table S1) and normalized to that of Actin150. Data are means ± se (n = 3). Statistical significance was determined by Student’s t-test. *, P < 0.05; **, P < 0.01