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Fig. 6 | BMC Plant Biology

Fig. 6

From: Transcription elongation factor AtSPT4-2 positively modulates salt tolerance in Arabidopsis thaliana

Fig. 6

Transcriptomic analyses reveal differentially expressed genes (DEGs) affected by AtSPT4-2. a Volcano plot of AtSPT4-2-mediated DEGs. The Y-axis represents the value of log2 (FC), and the X-axis represents the value of log2 (FPKM). Red dots represent upregulated genes, green dots represent downregulated genes, and black dots indicate those without significant changes. b Venn diagram of DEGs among the KO vs WT-normal/salt and OE vs WT-normal/salt groups. The number represents the overlapping numbers of DEGs in each of the groups. c Hierarchical clustering analyses of DEGs affected by AtSPT4-2. The heatmap represents the transcript abundance of DEGs in different comparison groups. Expression levels for genes are represented as colors ranging from yellow to blue. d The transcript levels of stress-responsive genes from c. Seven-day-old plants grown on MS medium were transferred to MS medium with 150 mM NaCl for 0, 3 hours, then RNA was isolated from the seedlings for qRT–PCR analyses of the indicated genes. UBQ5 was used as an internal control. Values are mean ± SD (n = 3 replicates, 30 seedlings per replicate). Different letters indicate significant differences by one-way ANOVA (P < 0.05)

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